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Book Cover

Title Apoptosis techniques and protocols
Edition 2nd ed. / edited by Andréa C. Leblanc
Published Totowa, N.J. : Humana, ©2002


Description 1 online resource (xv, 250 pages) : illustrations
Series Neuromethods, 0893-2336 ; 37
Neuromethods ; 37. 0893-2336
Contents Molecular characterization of the proapoptotic protein bax / Yi-Te Hsu and Soraya Smaili -- Assessing cytochrome-c release from mitochondria / Douglas W. Ethell and Douglas R. Green -- Monitoring caspases in neuronal cell death / Younes Bounhar, Omar Tounekti, and Andréa C. LeBlanc -- HSV amplicon vectors in neuronal apoptosis studies / Kathleen A. Maguire-Zeiss, William J. Bowers, and Howard J. Federoff -- Microinjections to study the specific role of proapoptotic proteins in neurons / Yan Zhang and Andréa C. LeBlanc -- Designing microarray experiments for neurobiology / P. Scott Eastman and Jeanne F. Loring -- Analysis of gene expression by nylon membrane cDNA arrays / H. Michael Tucker and Steven Estus -- Glyceraldehyde-3-phosphate dehydrogenase as a target for antiapoptotic drugs / Mark D. Berry and Paula C. Ashe -- Analytical approaches for investigating apoptosis and other biochemical events in compartmented cultures of sympathetic neurons / Barbara Karten [and others] -- Detection and analysis of synaptosis / Greg M. Cole and Karen Gylys -- In situ detection of apoptotic neurons / Kevin A. Roth -- Apoptotic and oxidative indicators in Alzheimer's Disease / Arun K. Raina [and others]
Summary The study of the apoptotic process has grown exponentially since the publication of the first edition of Apoptosis Techniques and Protocols for the Neuromethods series in 1997. In this greatly updated second edition, seasoned experts describe in step-by-step detail their best state-of-the-art techniques for studying neuronal cell death. These readily reproducible methods solve a wide variety of research problems, including the detection of the key proteins involved in neuronal apoptosis (Bax protein, cytochrome-c, and caspases), the direct assessment of the role of pro-apoptotic proteins in neurons by viral infections and microinjections, and the detection of proapoptotic proteins in situ. There are also hands-on methods for the study of apoptosis mechanisms in neuronal compartments, for studying synaptosis, and for establishing gene expression profiles in neurodegenerative brain tissues by using DNA microarrays. Written by investigators who have used the techniques extensively, each protocol includes tips on avoiding pitfalls, notes on the method's advantages and disadvantages, and a critical survey of the literature. Cutting-edge and highly practical, Apoptosis Techniques and Protocols, Second Edition, offers both novice and seasoned investigators a rich panoply of the productive tools they need to unravel the molecular mechanisms of neuronal cell death
Bibliography Includes bibliographical references and index
Notes English
UofL: Multi-user access (3 concurrent users)
Print version record
Subject Apoptosis -- Laboratory manuals
Apoptosis -- physiology
Brain Diseases -- physiopathology
Histocytological Preparation Techniques
Neurons -- physiology
SCIENCE -- Life Sciences -- Anatomy & Physiology.
Genre/Form Laboratory manuals
Laboratory manuals.
Manuels de laboratoire.
Form Electronic book
Author LeBlanc, Andréa C.
LC no. 2002513309
ISBN 1588290123