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Book Cover
Author Exbrayat, J. M

Title Genome visualization by classic methods in light microscopy / Jean-Marie Exbrayat
Published Boca Raton, FL : CRC Press, ©2001


Description 1 online resource (xiii, 195 pages) : illustrations (some color)
Series Methods in visualization
Methods in visualization
Contents GENERAL INTRODUCTION; ABBREVIATIONS; PRINCIPLES; Nucleic Acids; General Principles; TISSUE PREPARATION; Sampling; Fixation; Embedding; Sections; Adhesion of Sections; Deparaffining and Hydration; Collodioning; Smears; Cell Cultures; STAINING; Nuclear Dyes; Methods Used to Increase Contrast; HISTOCHEMICAL METHODS; General Methods; Basophilic Reactions; Feulgen and Rossenbeck Nuclear Reaction; Other Reactions; FLUORESCENT METHODS; Fluorescent Methods; Staining Methods; OBSERVATION PHASES; Mounting; Photonic Microscope; PREPARATION OF PRODUCTS; Fixatives; Dyes; Alcohol Dilutions; PROTOCOLS; Introduction; Fixation of Organs for Nucleic Acid Visualization; Staining; EXAMPLES OF STAINING METHODS; GLOSSARY; INDEX
Summary "Genome Visualization by Classic Methods in Light Microscopy covers the classic techniques used to visualize nucleic acids with the use of light microscopy. The book discusses the chemical structure of nucleic acids, general principles of staining, nuclear dyes, and histochemical methods. In addition, the book addresses issues pertaining to frozen sections, smears and quantitative analysis. Each chapter features the different methods of staining along with the relevant principles and protocols." "To facilitate the presentation of the practical and theoretical aspects, Genome Visualization by Classic Methods in Light Microscopy places them side by side in a convenient two-column format. The author also takes advantage of this format to provide valuable commentaries at each stage of the visualization process. The techniques described are general, and this feature renders the materials adaptable to all users of microscopic visualization of nucleic acids."--Jacket
Bibliography Includes bibliographical references and index
Subject Nucleic acids.
Gene expression.
Nucleic Acids -- ultrastructure
Gene Expression
Histocytochemistry -- methods
Microscopy -- methods
Nucleic Acids
SCIENCE -- Life Sciences -- Molecular Biology.
Gene expression
Nucleic acids
Acides nucléiques.
Microscopie -- Méthodologie.
Cytochimie -- Méthodologie.
Genre/Form Electronic book
Laboratory manuals
Laboratory manuals.
Manuels de laboratoire.
Form Electronic book
LC no. 00057198
ISBN 0849300436