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Title Histidine phosphorylation : methods and protocols / edited by Claire E. Eyers
Published New York, NY : Humana Press, [2020]


Description 1 online resource (1 PDF file (x, 255 pages)) : illustrations
Series Methods in molecular biology, 1940-6029 ; 2077
Springer protocols
Methods in molecular biology (Clifton, N.J.) ; v. 2077. 1064-3745
Springer protocols (Series) 1949-2448
Contents Protein Dynamics in Phosphoryl-transfer Signaling Mediated by Two-component Systems Felipe Trajtenberg and Alejandro Buschiazzo -- A High-throughput Strategy for Recombinant Protein Expression and Solubility Screening in Escherichia coli -- A Case of Sensor Histidine Kinase Agnieszka Szmitkowska, Blanka Pekárová, and Jan Hejátko -- SDS-PAGE and Dot Blot Autoradiography : Tools for Quantifying Histidine Kinase Autophosphorylation Jonathan T. Fischer, Ilana Heckler, and Elizabeth M. Boon -- A Quantitative Method for the Measurement of Protein Histidine Phosphorylation Paul V. Attwood -- Analysis of 1- and 3-phosphohistidine (pHis) Protein Modification using Model Enzymes Expressed in Bacteria Alice K.M. Clubbs Coldron, Dominic P. Byrne, and Patrick A. Eyers -- Determination of Phosphohistidine Stoichiometry in Histidine Kinases by Intact Mass Spectrometry Lauren J. Tomlinson, Alice K.M. Clubbs Coldron, Patrick A. Eyers, and Claire E. Eyers -- Protein Phosphohistidine Phosphatases of the HP Superfamily Daniel J. Rigden -- In Vitro Assays for Measuring Protein Histidine Phosphatase Activity Brandon S. McCullough and Amy M. Barrios -- Structural and Functional Characterization of Autophosphorylation in Bacterial Histidine Kinases Laura Miguel-Romero, Cristina Mideros-Mora, Alberto Marina, and Patricia Casino -- Manipulation of Bacterial Signalling using Engineered Histidine Kinases Kimberly A. Kowallis, Samuel W. Duvall, Wei Zhao, and W. Seth Childers -- Development of a Light-Dependent Protein Histidine Kinase Aleksandra Bury and Klaas J. Hellingwerf -- Empirical Evidence of Cellular Histidine Phosphorylation by Immunoblotting using pHis mAbs Rajasree Kalagiri, Kevin Adam, and Tony Hunter -- Immunohistochemistry (IHC): Chromogenic Detection of 3-Phosphohistidine Proteins in Formaldehyde-Fixed, Frozen Mouse Liver Tissue Sections Natalie Luhtala and Tony Hunter -- Subcellular Localisation of Histidine Phosphorylated Proteins through Indirect Immunofluorescence Kevin Adam and Tony Hunter -- High throughput Characterisation of Histidine Phosphorylation sites using UPAX and Tandem Mass Spectrometry Gemma Hardman and Claire E. Eyers -- Proteome Bioinformatics Methods for Studying Histidine Phosphorylation Andrew R Jones and Oscar Martin Camacho
Bibliography Includes bibliographical references and index
Notes Current copyright fee: GBP19.00 42\0. Uk
Online resource; title from PDF title page (SpringerLink, viewed November 14, 2019)
Subject Phosphorylation.
Amino acids.
Histidine Kinase
Immunohistochemistry -- methods
Amino acids
Genre/Form Laboratory manuals
Laboratory manuals.
Manuels de laboratoire.
Form Electronic book
Author Eyers, Claire E., editor.
ISBN 9781493998845