Limit search to available items
Book Cover

Title Molecular morphology in human tissues : techniques and applications / edited by Gerhard W. Hacker, Raymond R. Tubbs
Published Boca Raton : CRC Press, ©2005


Description 1 online resource (xxii, 304 pages) : illustrations
Series Advances in pathology, microscopy & molecular morphology
Advances in pathology, microscopy & molecular morphology.
Contents Cover Advances in Pathology, Microscopy, & Molecular Morphology MOLECULAR MORPHOLOGY in HUMAN TISSUES: Techniques and Applications DEDICATION FOREWORD PREFACE THE EDITORS ACKNOWLEDGMENTS CONTRIBUTORS CONTENTS 1 Immunostaining Techniques for Co -- Localization of Multiple Peptide Antigens in Light Microscopy 1.1 INTRODUCTION 1.2 SINGLE IMMUNOSTAINING ON CONSECUTIVE SECTIONS 1.3 DOUBLE OR TRIPLE IMMUNOSTAINING ON THE SAME SECTION 1.4 QUADRUPLE IMMUNOSTAINING ON THE SAME SECTION 1.5 CONCLUSIONS 1.6 PROTOCOLS I, II, III, IV, V, AND VI Acknowledgments References 2 Multiple Staining in Molecular Morphology 2.1 INTRODUCTION 2.2 DOUBLE STAINING CONCEPTS 2.3 MULTIPLE STAINING STRATEGY AND CONTROLS 2.4 SELECTING A CONCEPT, DETECTION SYSTEM, AND COLOR COMBINATION FOR DOUBLE STAINING 2.5 ADDITIONAL PRACTICAL TIPS AND COMMON MISTAKES WITH DOUBLE STAINING 2.6 PROTOCOLS AND TECHNICAL NOTES References ABBREVIATIONS 3 Enzyme-Based Fluorescence Amplification for Immunohistochemistry and In Situ Hybridization 3.1 INTRODUCTION 3.2 PROTOCOLS 3.3 RESULTS 3.4 TECHNICAL HINTS AND DISCUSSION Acknowledgments References 4 Gold Cluster Labels and Related Technologies in Molecular Morphology 4.1 INTRODUCTION: WHY CLUSTER LABELS? 4.2 CLUSTER LABELING METHODS AND RELATED TECHNOLOGIES 4.3 SPECIFIC GOLD CLUSTER PROBES AND METHODS References 5 Gold- and Silver-Facilitated Metallographic In Situ Hybridization Procedures for Detection of HER2 Gene Amplification 5.1 INTRODUCTION 5.2 FIXATION AND TISSUE PROCESSING 5.3 MATERIALS AND PROTOCOL PROCEDURE References 6 Toward Molecular Sensitivity: Tyramide Signal Amplification in Molecular Morphology 6.1 INTRODUCTION AND BRIEF HISTORICAL OVERVIEW 6.2 TYRAMIDE-AMPLIFIED NANOGOLD-SILVER/GOLD ISH 6.3 DISCUSSION 6.4 OUTLOOK Acknowledgments References 7 Array-Based Comparative Genomic Hybridization as a Tool for Survey of Genomic Alterations in Human Neoplasms 7.1 INTRODUCTION 7.2 MATERIALS AND PROTOCOLS 7.3 TROUBLESHOOTING References 8 Southwestern Histochemistry: A Method for Localization of Transcription Factors 8.1 INTRODUCTION 8.2 PROTOCOL A: FROZEN TISSUE SECTIONS 8.3 PROTOCOL B: PARAFFIN-EMBEDDED TISSUE SECTIONS 8.4 PROTOCOL C: CULTURED CELLS 8.5 RESULTS 8.6 TECHNICAL HINTS AND DISCUSSION Acknowledgments References 9 High Throughput Morphological Gene Expression Studies Using Automated mRNA In Situ Hybridization Applications and Tissue Microarrays for Post-Genomic and Clinical Research 9.1 INTRODUCTION 9.2 IMMUNOHISTOCHEMISTRY VS. ISOTOPIC MRNA ISH APPLICATIONS 9.3 AUTOMATED COLORIMETRIC MRNA ISH TECHNOLOGY 9.4 TISSUE MICROARRAYS 9.5 PROTOCOLS 9.6 RESULTS 9.7 TECHNICAL HINTS AND DISCUSSION 9.8 KEY FACTORS FOR SUCCESSFUL MRNA ISH EXPERIMENTS References 10 Whole-Mount In Situ Hybridization: Manual and Automated Procedure 10.1 INTRODUCTION 10.2 MATERIALS AND METHODS 10.3 TECHNICAL HINTS AND DISCUSSION References 11 An Open Door to Molecular Cytopathology: Interphase Fluorescence In Situ Hybridization (FISH) on Liquid-Based Thin-Layer Preparations 11.1 INTRODUCTION 11.2 TECHNICAL ASPECTS OF FISH 11.3 LIQUID-BASED THIN-LAYER PREPARATION AND ITS SUITABILITY FOR FISH 11.4 MATERIALS AND PROCEDURE 11.5 PITFALLS AND TROUBLESHOOTING 11.6 CONCLUSIONS References 12 Recent Developments in In Situ PCR 12.1 INTRODUCTION 12.2 REVIEW OF THE PCR TECHNIQUE 12.3 PRACTICAL PROTOCOLS References 13 High-Resolution Visualization of Apoptotic Markers in Human Biopsies 13.1 INTRODUCTION 13.2 MATERIAL AND REAGENTS 13.3 SECTIONING AND PREPARATION OF SLIDES 13.4 STAINING PROCEDURE 13.5 RESULTS 13.6 DISCUSSION References 14 Three-Dimensional Full Color Demonstration of Bright-Field and Fluorescence Microscopic Preparations: The Digital Optical Microscope 14.1 INTRODUCTION 14.2 METHODOLOGY 14.3 APPLICATIONS AND DISCUSSION 14.5 THE FUTURE References 15 Visual
Summary Molecular Morphology in Human Tissues: Techniques and Applications presents the most advanced molecular morphological techniques to date. This integrated approach to molecular morphology provides powerful analytical and diagnostic tools at the genome level, making the diagnosis and management of cancer, viral infections, and other diseases more precise and reliable. The pioneers of these techniques contribute step-by-step protocols with tips, guidelines, and other suggestions from their own notebooks, enabling experienced scientists as well as beginners to apply the most progressive detection
Bibliography Includes bibliographical references and index
Notes Print version record
Subject Histochemistry -- Laboratory manuals
Histocytological Preparation Techniques
Immunohistochemistry -- methods
MEDICAL -- Histology.
Genre/Form Electronic books
Laboratory manuals.
Form Electronic book
Author Hacker, Gerhard W
Tubbs, Raymond R., 1946-
ISBN 0203503481