| Description |
1 online resource (222 pages) |
| Contents |
Cover; Further Titles for Chromatographers; Title Page; Copyright; Preface; Introduction; Part I: Fundamentals; 1.1 Chromatography; 1.2 Chromatographic Figures of Merit; 1.3 The Resolution of Two Peaks; 1.4 Reduced Parameters; 1.5 The Van Deemter Curve; 1.6 Peak Capacity and Number of Possible Peaks; 1.7 Statistical Resolution Probability: Simulation; 1.8 Statistical Resolution Probability: Example; 1.9 Precision and Accuracy of an Analytical Result; 1.10 Standard Deviation; 1.11 Variability of the Standard Deviation; 1.12 Uncertainty Propagation; 1.13 Reproducibility in Trace Analysis |
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1.14 Ruggedness1.15 Calibration Curves; 1.16 The HPLC Instrument; 1.17 The Detector Response Curve; 1.18 Noise; 1.19 The Playground Presented as an Ishikawa Diagram; 1.20 The Possible and the Impossible; Part II: Pitfalls and Sources of Error; 2.1 Mixing of the Mobile Phase; 2.2 Mobile Phase pH; 2.3 Adjustment of Mobile Phase pH; 2.4 Influence of the Acid Type and Concentration in the Eluent; 2.5 Water as an Unintentional Additive in the Mobile Phase; 2.6 Inadequate Purity of Mobile Phase Water; 2.7 Inadequate Purity of a Mobile Phase Solvent; 2.8 Inadequate Purity of a Mobile Phase Reagent |
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2.22 Injection Technique2.23 Injection of Air; 2.24 Sample Adsorption in the Loop; 2.25 Extra-Column Volumes; 2.26 Dwell Volume; 2.27 Elution at t0; 2.28 Classification of C18 Reversed Phases; 2.29 Different Selectivity of C18 Reversed Phases; 2.30 Different Batches of Stationary Phase; 2.31 Chemical Reaction within the Column; 2.32 Tailing of Phosphate Compounds in the Presence of Steel; 2.33 Recovery and Peak Shape Problems with Proteins; 2.34 Double Peaks from Stable Conformers; 2.35 Influence of Temperature on the Separation; 2.36 Thermal Non-Equilibrium within the Column |
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2.37 Influence of the Flow Rate on the Separation2.38 Influence of Run Time and Flow Rate on Gradient Separations; 2.39 UV Spectra and Quantitative Analysis; 2.40 UV Detection Wavelength; 2.41 Different Detection Properties of Diastereomers; 2.42 Fluorescence Quenching by Air; 2.43 Detector Overload in UV; 2.44 Detector Overload in ELSD; 2.45 Influence of the Retention Factor on Peak Height; 2.46 Influence of the Flow Rate on Peak Area; 2.47 Leaks in the HPLC Instrument; 2.48 Impairment of Precision as a Result of Noise; 2.49 Determination of Peak Area and Height at High Noise |
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2.9 Incomplete Degassing2.10 System Peaks and Quantitative Analysis; 2.11 Sample Preparation with Solid Phase Extraction; 2.12 Inadequate Stabilization of the Extraction Solvent; 2.13 Poor Choice of Sample Solvent: Peak Distortion; 2.14 Poor Choice of Sample Solvent: Tailing; 2.15 Sample Solvent and Calibration Curve; 2.16 Impurities in the Sample; 2.17 Formation of a by-Product in the Sample Solution; 2.18 Decomposition by the Sample Vial; 2.19 Artifact Peaks from the Vial Septum; 2.20 Formation of an Associate in the Sample Solution; 2.21 Precision and Accuracy with Loop Injection |
| Summary |
The third edition of this popular problem-solving guide for this widely-used method includes eleven completely new examples and several updated ones, adding up to over 100 potential pitfalls and errors in HPLC. Each example is presented on a double page with the text on the left-hand and a figure on the right-hand side, true to the motto ""a picture says more than a thousand words"". In addition, the author presents essential fundamentals as well as helpful strategies, such as equipment tests or quality assurance processes. A must-have resource for all those users without a background in an |
| Notes |
2.50 Peak Height Ratios |
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Print version record |
| Subject |
Errors, Scientific -- Prevention.
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High performance liquid chromatography -- Industrial applications.
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High performance liquid chromatography.
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| Form |
Electronic book
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| ISBN |
3527659129 |
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9783527659128 |
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9783527659104 |
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