Title Antiviral methods and protocols / edited by Derek Kinchington and Raymond F. Schinazi

Published Totowa, N.J. : Humana Press, ©2000

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Description 1 online resource (xiv, 404 pages) : illustrations Series Methods in molecular medicine ; 24 Methods in molecular medicine ; 24. Contents Changing methods for discovering antiviral drugs / P.S. Jones-Laboratory safety considerations / P.M. Feorino, J.D. Williamson and R.F. Schinazi-Routine quality control testing of cell cultures : Detection of mycoplasma / A. Stacey and G. Stacey-Hep ad38 assay : A high-throughput, cell-based screen for the evaluation of compounds against hepatitis b virus / R.W. King and S.K. Ladner-Hepatitis b virus cell culture assays for antiviral activity / K. Schmidt and B. Korba-A rapid microtiter assay for duck hepatitis virus reverse transcriptase / M.J. Otto and R.A. Whitaker-Assays for measuring hepadnaviral supercoiled DNA / S.A. Locamini and G.M. Civitico-Assays for hepatitis b virus DNA-and rna-dependent DNA polymerase activities / T. Shaw and S.A. Locarnini-Development of a hepatitis c virus rna helicase high throughput assay / A.D. Kwong and C. Risano-Determination of viral infectivity : Hsv, vzv, and cmv / D.R. Harper-Antiviral screening assays for human herpesviruses 6 and 7 / J.B. Black and P.E. Pellett-Strategies for evaluation of antiviral agents against epstein-barr virus in culture / J.C. Lin-Monitoring drug resistance for herpesviruses / G. Andrei [and others]-Herpesvirus protease assays / P. Ertl, L. Russell and J. Angier-Testing compounds for antiviral activity in cell cultures infected with hiv / D. Devine, N. Mathews and D. Kinchington-Evaluation of compounds that prevent reactivation of hiv-1 in om-10.1 cells / S.T. Butera-Assessment of activity of topical virucidal agents / O.C. T-Assays that measure selective killing of virus-infected cells / S. Randall-Evaluating clinical isolates for their phenotypic and genotypic resistance against anti-hiv drugs / A.M. Vandamme [and others]-Line probe assay for detecting mutations in hiv-1 reverse transcriptase / L. Stuyver [and others]-Rt-pcr for amplification of specific fragments of hiv-1 genome / M.P. de Bethune and K. Hertogs-Evaluation of compounds against recombinant hiv reverse transcriptase / J. Balzarini-Ultrasensitive detection of reverse transcriptase activity by the amp-rt assay : Applications to the measurement of virus loads and phenotypic drug resistance / J. Gerardo, L. Garcia and W. Heneine-Reverse transcriptase assay based on product enhancement for assessing the drug susceptibility of retroviruses / J. Boni and J. Schupbach-Identifying and characterizing hiv protease inhibitors / E.S. Furfine-Retroviral integrase : A novel target in antiviral drug development and basic in vitro assays with the purified enzyme / A. Mazumder [and others]-Papillomavirus DNA replication / M.C. Ferran and A.A. McBride-Quantitative immunodetection of viral oncoprotein levels in human papillomavirus-transformed human cells / J.A. Johnson and J.D. Gangemi-Biochemical methods for the characterization of influenza viruses with reduced sensitivity to 4-guanidino-neu5ac2en / R.C. Bethell [and others]-Virological methods for the generation and characterization of influenza viruses with reduced sensitivity to 4-guanidino-neu5ac2en / J.L. McKimm-Breschkin [and others] Summary In Antiviral Methods and Protocols, experienced scientists describe-in an easily-followed format-their state-of-the-art techniques for evaluating antiviral compounds. The assays described include systems for investigating drugs used against herpesviruses, hepatitis viruses, human immunodeficiency viruses, human papillomaviruses, and influenza viruses. These well-tested methods range from cellular assays to some of the most up-to-date molecular approaches for identifying compounds that are active against viral enzymes and the development of viral resistance against drugs currently in use. The detailed protocols also discuss possible pitfalls and ways to overcome them. Timely and comprehensive, Antiviral Methods and Protocols offers today's researchers in academia, clinical departments, and the pharmaceutical industry the powerful, reproducible, and novel methods needed to evaluate compounds effective against both acute and chronic infections Analysis antivirale middelen antiviral agents antiviruseigenschappen antiviral properties geneesmiddelen drugs Pharmacology Farmacologie Bibliography Includes bibliographical references and index Notes Master and use copy. Digital master created according to Benchmark for Faithful Digital Reproductions of Monographs and Serials, Version 1. Digital Library Federation, December 2002. http://purl.oclc.org/DLF/benchrepro0212 MiAaHDL English Print version record digitized 2010 HathiTrust Digital Library committed to preserve pda MiAaHDL In Springer Protocols Subject Antiviral agents -- Laboratory manuals Hepatitis, Viral, Human -- drug therapy Antiviral Agents -- therapeutic use DNA Virus Infections -- drug therapy RNA Virus Infections -- drug therapy MEDICAL -- Infectious Diseases. Antiviral agents Aufsatzsammlung Medizin Molekularbiologie Viren Wetenschappelijke technieken. Genre/Form Handbooks and manuals Laboratory manuals. Manuels de laboratoire. Form Electronic book Author Kinchington, Derek Schinazi, Raymond F ISBN 9781592592456 0585235686 9780585235684 1592592457 1280831367 9781280831362

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