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Title Hematopoietic stem cell protocols / edited by Kevin D. Bunting
Edition 2nd ed
Published Totowa, N.J. : Humana Press, ©2008

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Description 1 online resource (xiv, 312 pages) : illustrations
Series Methods in molecular biology ; v. 430
Methods in molecular biology (Clifton, N.J.) ; v. 430
Contents The expanding tool kit for hematopoietic stem cell research / William Tse and Kevin D. Bunting -- Isolation of quiescent murine hematopoietic stem cells by homing properties / Tarja A. Juopperi and Saul J. Sharkis -- Mobilization of hematopoietic stem and progenitor cells in mice / Simon N. Robinson and Ronald P. van Os -- Transmigration of human CD34+ cells / Seiji Fukuda and Louis M. Pelus -- Flow cytometry-based cell cycle measurement of mouse hematopoietic stem and progenitor cells / Hongmei Shen, Matthew Boyer, and Tao Cheng -- Analysis of apoptosis in hematopoietic stem cells by flow cytometry / William G. Kerr -- In vitro hematopoietic differentiation of murine embryonic stem cells / Jinhua Shen and Cheng-Kui Qu -- Hematopoietic development of human embryonic stem cells in culture / Xinghui Tian and Dan S. Kaufman -- In vitro human T cell development directed by notch-ligand interactions / Génève Awong, Ross N. La Motte-Mohs, and Juan Carlos Zuñiga-Pflücker -- In vitro assays for cobblestone area-forming cells, LTC-IC, and CFU-C / Ronald P. van Os, Bertien Dethmers-Ausema, and Gerald de Haan -- Hematopoietic stem cell transplant in mice by intra-femoral injection / Yuxia Zhan and Yi Zhao -- Hematopoietic stem cell transplant into non-myeloblated W/Wv mice to detect steady-state engraftment defects / Zhengqi Wang and Kevin D. Bunting -- Isolation and functional characterization of side population stem cells / Jonathan B. Johnnidis and Fernando D. Camargo -- Transplanatation of chimeric fetal liver to study hematopoiesis / Sigrid Eckardt and K. John McLaughlin -- Immunodeficient mouse models to study human stem cell-mediated tissue repair / Ping Zhou [and others] -- Retroviral transduction of murine hematopoietic stem cells / Peter Haviernik, Yi Zhang, and Kevin D. Bunting -- Lentiviral gene transduction of mouse and human stem cells / Zhaohui Ye, Xiaobing Yu, and Linzhao Cheng -- Retroviral integration site analysis in hematopoietic stem cells / Olga S. Kustikova, Christopher Baum, and Boris Fehse -- A detailed protocol for bacterial artificial chromosome recombineering to study essential genes in stem cells / Andriy Tsyrulnyk and Richard Moriggl -- Bioluminescence imaging of hematopoietic stem cell repopulation in murine models / Yuan Lin [and others]
Summary With new technologies emerging and old technologies being reimagined, the field of hematopoietic stem cell (HSC) research continues to grow rapidly in pursuit of improved therapies for many blood diseases. Updating and building upon the thorough first edition's foundation, Hematopoietic Stem Cell Protocols, Second Edition provides up-to-date protocols developed in the HSC field. A team of leading researchers supply this volume with in-depth, readily reproducible methods for effective characterization of HSC and their developmental potential. An introductory chapter also hints at potential techniques that may develop in the future. Following the format of the successful Methods in Molecular Biology series, each protocol offers step-by-step laboratory instructions, lists of the necessary equipment, and tips on troubleshooting and avoiding known pitfalls. Essential for the laboratory-based researcher, Hematopoietic Stem Cell Protocols, Second Edition is a much needed technical resource in the critically important field of stem cell investigation
Analysis bloedstoornissen
blood disorders
cellen
cells
protocollen
protocols
Human Pathology
Cellular Biology
Humane pathologie
Celbiologie
Bibliography Includes bibliographical references and index
Notes English
Print version record
In Springer Protocols
Subject Hematopoietic stem cells.
Genetic engineering.
Cytological Techniques -- methods
Hematopoietic Stem Cells -- cytology
Hematopoietic Stem Cells -- physiology
Hematopoietic Stem Cells
Genetic engineering
Hematopoietic stem cells
Células madre hematopoyéticas.
Ingeniería genética.
Genre/Form Laboratory manuals
Laboratory manuals.
Manuels de laboratoire.
Form Electronic book
Author Bunting, Kevin D
ISBN 9781597451826
1597451827