| Description |
1 online resource (xii, 364 pages) : illustrations (some color) |
| Series |
Methods in molecular biology, 1940-6029 ; v. 1185 |
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Methods in molecular biology (Clifton, N.J.) ; v. 1185. 1064-3745
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| Contents |
PART I. OVERVIEW -- 1. The Hematopoietic Stem Cell Landscape / Kevin D. Bunting and Cheng-Kui Qu |
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PART II. STEM CELL ENRICHMENT AND HETEROGENEITY -- 2. Investigating the Interaction Between Hematopoietic Stem Cells and Their Niche During Embryonic Development: Optimizing the Isolation of Fetal and Newborn Stem Cells From Liver, Spleen, and Bone Marrow / Huimin Cao, Brenda Williams, and Susan K. Nilsson -- 3. Single-Cell PCR Profiling of Gene Expression in Hematopoiesis / José Teles, Tariq Enver, and Cristina Pina -- 4. Hematopoietic Stem and Progenitor Cell Mobilization in Mice / Jonathan Hoggatt, Tiffany A. Tate, and Louis M. Pelus -- 5. Cell Cycle Measurement of Mouse Hematopoietic Stem/Progenitor Cells / Brahmananda Reddy Chitteti and Edward F. Srour -- 6. Flow Cytometric Analysis of Signaling and Apoptosis in Hematopoietic Stem Cells / Lei Dong and Cheng-Kui Qu |
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PART III. MOLECULAR AND CELLULAR CHARACTERIZATION -- 7. Gene Expression Profiling of Hematopoietic Stem Cells (HSCs) / Nalini Raghavachari -- 8. Measuring MicroRNA Expression in Mouse Hematopoietic Stem Cells / Wenhuo Hu and Christopher Y. Park -- 9. DNA Methylation Profiling of Hematopoietic Stem Cells / Amber Hogart Begtrup -- 10. Metabolic Characterization of Hematopoietic Stem Cells / Fatih Kocabas, Junke Zheng, Chengcheng Zhang, and Hesham A. Sadek -- 11. Nanoproteomic Assays on Hematopoietic Stem Cells / Heath L. Bradley, Himalee Sabnis, Deborah Pritchett, and Kevin D. Bunting |
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PART IV. IN VITRO ASSAYS AND DIFFERENTIATION -- 12. Hematopoietic Differentiation of Pluripotent Stem Cells in Culture / Jason A. Mills, Prasuna Paluru, Mitchell J. Weiss, Paul Gadue, and Deborah L. French -- 13. Ex Vivo Assays to Study Self-Renewal, Long-Term Expansion, and Leukemic Transformation of Genetically Modified Human Hematopoietic and Patient-Derived Leukemic Stem Cells / Pallavi Sontakke, Marco Carretta, Marta Capala, Hein Schepers, and Jan Jacob Schuringa -- 14. Ex Vivo Expansion of Murine and Human Hematopoietic Stem Cells / Phuong L. Doan and John P. Chute -- 15. Using Microfluidics to Investigate Hematopoietic Stem Cell and Microniche Interactions at the Single Cell Level / Byungwook Ahn, Zhengqi Wang, David R. Archer, and Wilbur A. Lam |
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PART V. TRANSPLANTATION ASSAYS AND IMAGING ENGRAFTMENT -- 16. Five-Lineage Clonal Analysis of Hematopoietic Stem/Progenitor Cells / Ryo Yamamoto, Yohei Morita, and Hiromitsu Nakauchi -- 17. Intravital Imaging of Hematopoietic Stem Cells in the Mouse Skull / Juwell W. Wu, Judith M. Runnels, and Charles P. Lin -- 18. Immunodeficient Mouse Model for Human Hematopoietic Stem Cell Engraftment and Immune System Development / Ken-Edwin Aryee, Leonard D. Shultz, and Michael A. Brehm -- 19. Homing and Migration Assays of Hematopoietic Stem/Progenitor Cells / Xi C. He, Zhenrui Li, Rio Sugimura, Jason Ross, Meng Zhao, and Linheng Li |
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PART VI. GENETIC MODIFICATION -- 20. Retroviral Transduction of Murine and Human Hematopoietic Progenitors and Stem Cells / Marioara F. Ciuculescu, Christian Brendel, Chad E. Harris, and David A. Williams -- 21. Lentiviral Gene Transduction of Mouse and Human Hematopoietic Stem Cells / Niek P. van Til and Gerard Wagemaker -- 22. High-Throughput Genomic Mapping of Vector Integration Sites in Gene Therapy Studies / Brian C. Beard, Jennifer E. Adair, Grant D. Trobridge, and Hans-Peter Kiem -- 23. Barcoded Vector Libraries and Retroviral or Lentiviral Barcoding of Hematopoietic Stem Cells / Leonid V. Bystrykh, Gerald de Haan, and Evgenia Verovskaya |
| Summary |
"Includes cutting-edge methods and protocols. Provides step-by-step detail essential for reproducible results. Contains key notes and implementation advice from the experts. Updating and building upon previous editions, Hematopoietic Stem Cell Protocols, Third Edition provides up-to-date protocols from leading stem cell researchers. This in-depth volume presents a clear view of the landscape of assays available to the stem cell researcher working in the growing hematopoietic stem cell (HSC) field. A robust and active field, it is supported by an abundance of innovative mouse models and molecular tools for analysis of phenotypes and functions in mouse and human cells. Understanding more about hematopoietic stem cell biology is integral if these versatile cells are to be applied effectively to treat and cure a wide range of blood diseases. An introductory chapter puts the major contributions of the book into the proper perspective. Written in the successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible protocols, and notes on troubleshooting and avoiding known pitfalls. Essential for the laboratory-based researcher, Hematopoietic Stem Cell Protocols, Third Edition is a much needed technical resource in the critically important field of hematopoietic stem cell investigation."--Publisher's description |
| Analysis |
celbiologie |
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cellular biology |
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stamcellen |
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stem cells |
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Medicine (General) |
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Geneeskunde (algemeen) |
| Bibliography |
Includes bibliographical references and index |
| Notes |
Previous edition: 2008 |
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Online resource. Springer Protocols (viewed September 11, 2014) |
| Subject |
Hematopoietic stem cells -- Laboratory manuals
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Genetic engineering -- Laboratory manuals
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Hematopoietic Stem Cells -- cytology
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Cytological Techniques -- methods
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Hematopoietic Stem Cells -- physiology
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Hematopoietic stem cells
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Genetic engineering
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Biology -- Laboratory manuals
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| Genre/Form |
Laboratory manuals.
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Manuels de laboratoire.
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| Form |
Electronic book
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| Author |
Bunting, Kevin D., editor
|
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Qu, Cheng-Kui, editor
|
| ISBN |
9781493911332 |
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1493911333 |
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1493911325 |
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9781493911325 |
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