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Title Hematopoietic stem cell protocols / edited by Christopher A. Klug and Craig T. Jordan
Published Totowa, N.J. : Humana Press, ©2002

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Description 1 online resource (xi, 332 pages) : illustrations
Series Methods in molecular medicine ; 63
Methods in molecular medicine ; 63.
Contents Isolation and analysis of hematopoietic stem cells from mouse embryos / E. Dzierzak -- The purification of mouse hematopoietic stem cells at sequential stages of maturation / S.J. Morrison -- Flow cytometry and immunoselection of human stem cells / T.E. Thomas -- Method for purification of human hematopoietic stem cells by flow cytometry / M.J. Reitsma -- Noninvasive measurement of hematopoietic stem cell cycling and turnover by in vivo bromodeoxyuridine incorporation / G.G. Bradford -- Isolation and characterization of primitive hematopoietic cells based on their position in the cell cycle / E.F. Srour -- Hematopoietic colony-forming cells / M. Ogawa -- Long-term culture-initiating cell assays for human and murine cells / C.L. Miller -- The cobblestone-area-forming cell assay / G. Haan -- CFU-S: an assay for pluripotent myelopoietic stem cells / E.A. McCulloch -- Intrathymic injection for analysis of T-cell progenitor activity / L. Jerabek -- Quantitation of murine and human hematopoietic stem cells by limiting dilution analysis in competitively repopulated hosts / S.J. Szilvassy -- Ex vivo expansion of human and murine hematopoietic stem cells / C.L. Miller -- Hematopoietic development of ES cells in culture / G.M. Keller -- Genetic modification of murine hematopoietic stem cells by retroviruses / C.P. Kalberer -- Retroviral transduction of FACS-purified hematopoietic stem cells / C.V. Cotta -- Retroviral-mediated transduction and clonal integration analysis of human hematopoietic stem and progenitor cells / M.A. Dao -- Production of lentiviral vector supernatants and transduction of cellular targets / R.E. Sutton -- Reverse transcriptase-PCR analysis of gene expressiong in hematopoietic stem cells / D. Orlic -- Identification of differentially expressed genes in sorted cell populations by two-dimensional gene-expression fingerprinting / A.V. Belyavsky
Summary Hematopoietic stem cells have an enormous clinical importance for bone marrow transplantation, hematopoietic gene therapy, solid organ transplantation, and somatic tissue regeneration. In Hematopoietic Stem Cell Protocols, a group of leading stem-cell investigators describe in detail the key laboratory methods for investigating this critically important cell type. Varied in scope, and richly detailed with step-by-step instructions and notes on the pitfalls to be avoided, these readily reproducible protocols apply hematopoietic stem cells isolated from mouse and human tissues of both embryonic and adult origin. The many approaches described employ not only molecular and cell biology techniques, but also animal model systems, and include hematopoietic stem cell expansion protocols and methods for the purification and genetic modification of stem cells. Many of the protocols are contributed by those researchers who first developed and introduced the procedures to the scientific community. Comprehensive and state-of-the-art, Hematopoietic Stem Cell Protocols offers both novice and experienced investigators alike an invaluable compendium of highly practical techniques for studying a wide variety of stem cell topics, including blood formation, blood-based disease, and such blood cancers as leukemia
Bibliography Includes bibliographical references and index
Notes English
Print version record
In Springer Protocols
Subject Hematopoietic stem cells -- Laboratory manuals
Hematopoietic Stem Cells -- cytology
Cytological Techniques -- methods
Hematopoietic Stem Cells -- physiology
SCIENCE -- Life Sciences -- Human Anatomy & Physiology.
MEDICAL -- Anatomy.
Hematopoietic stem cells
Stamcellen.
Bloedvorming.
Wetenschappelijke technieken.
Genre/Form Handbooks and manuals
Laboratory manuals.
Manuels de laboratoire.
Form Electronic book
Author Klug, Christopher A.
Jordan, Craig T.
ISBN 9781592591404
0896038122
9780896038127
159259140X
1280821701
9781280821707
9786610821709
6610821704