| Description |
1 online resource (xii, 266 pages) : illustrations (some color) |
| Series |
Methods in Molecular Biology, Methods and Protocols, 1940-6029 ; Volume No. 1181 |
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Methods in molecular biology (Clifton, N.J.) ; v. 1181. 1064-3745
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| Contents |
1. Second Generation Codon Optimized Minicircle (CoMiC) for Nonviral Reprogramming of Human Adult Fibroblasts / Sebastian Diecke, Leszek Lisowski, Nigel G. Kooreman, and Joseph C. Wu -- 2. Scalable Cardiac Differentiation of Human Pluripotent Stem Cells as Microwell-Generated, Size Controlled Three- Dimensional Aggregates / Celine L. Bauwens and Mark D. Ungrin -- 3. Preparation and Characterization of Circulating Angiogenic Cells for Tissue Engineering Applications / Aleksandra Ostojic, Suzanne Crowe, Brian McNeill, Marc Ruel, and Erik J. Suuronen -- 4. Isolation and Expansion of C-Kit-Positive Cardiac Progenitor Cells by Magnetic Cell Sorting / Kristin M. French and Michael E. Davis -- 5. Synthesis of Aliphatic Polyester Hydrogel for Cardiac Tissue Engineering / Sanjiv Dhingra, Richard D. Weisel, and Ren-Ke Li -- 6. Fabrication of PEGylated Fibrinogen: A Versatile Injectable Hydrogel Biomaterial / Iris Mironi-Harpaz, Alexandra Berdichevski, and Dror Seliktar -- 7. Natural Cardiac Extracellular Matrix Hydrogels for Cultivation of Human Stem Cell-Derived Cardiomyocytes / Donald O. Freytes, John D. O'Neill, Yi Duan-Arnold, Emily A. Wrona, and Gordana Vunjak-Novakovic -- 8. Magnetically Actuated Alginate Scaffold: A Novel Platform for Promoting Tissue Organization and Vascularization / Yulia Sapir, Emil Ruvinov, Boris Polyak, and Smadar Cohen -- 9. Shrink-Induced Biomimetic Wrinkled Substrates for Functional Cardiac Cell Alignment and Culture / Nicole Mendoza, Roger Tu, Aaron Chen, Eugene Lee, and Michelle Khine -- 10. Injectable ECM Scaffolds for Cardiac Repair / Todd D. Johnson, Rebecca L. Braden, and Karen L. Christman -- 11. Generation of Strip-Format Fibrin-Based Engineered Heart Tissue (EHT) / Sebastian Schaaf, Alexandra Eder, Ingra Vollert, Andrea Stöhr, Arne Hansen, and Thomas Eschenhagen -- 12. Cell Tri-Culture for Cardiac Vascularization / Ayelet Lesman, Lior Gepstein, and Shulamit Levenberg -- 13. Cell Sheet Technology for Cardiac Tissue Engineering / Yuji Haraguchi, Tatsuya Shimizu, Katsuhisa Matsuura, Hidekazu Sekine, Nobuyuki Tanaka, Kenjiro Tadakuma, Masayuki Yamato, Makoto Kaneko, and Teruo Okano -- 14. Design and Fabrication of Biological Wires / Jason W. Miklas, Sara S. Nunes, Boyang Zhang, and Milica Radisic -- 15. Collagen-Based Engineered Heart Muscle / Malte Tiburcy, Tim Meyer, Poh Loong Soong, and Wolfram-Hubertus Zimmermann -- 16. Creation of a Bioreactor for the Application of Variable Amplitude Mechanical Stimulation of Fibrin Gel-Based Engineered Cardiac Tissue / Kathy Y. Morgan and Lauren D. Black III -- 17. Preparation of Acellular Myocardial Scaffolds with Well- Preserved Cardiomyocyte Lacunae, and Method for Applying Mechanical and Electrical Simulation to Tissue Construct / Bo Wang, Lakiesha N. Williams, Amy L. de Jongh Curry, and Jun Liao -- 18. Patch-Clamp Technique in ESC-Derived Cardiomyocytes / Jie Liu and Peter H. Backx -- 19. Optogenetic Control of Cardiomyocytes via Viral Delivery / Christina M. Ambrosi and Emilia Entcheva -- 20. Methods for Assessing the Electromechanical Integration of Human Pluripotent Stem Cell-Derived Cardiomyocyte Grafts / Wei-Zhong Zhu, Dominic Filice, Nathan J. Palpant, and Michael A. Laflamme -- 21. Quantifying Electrical Interactions Between Cardiomyocytes and Other Cells in Micropatterned Cell Pairs / Hung Nguyen, Nima Badie, Luke McSpadden, Dawn Pedrotty, and Nenad Bursac |
| Summary |
Annotation Cardiac Tissue Engineering: Methods and Protocols presents a collection of protocols on cardiac tissue engineering from pioneering and leading researchers around the globe. These include methods and protocols for cell preparation, biomaterial preparation, cell seeding, and cultivation in various systems. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and key tips on troubleshooting and avoiding known pitfalls. Authoritative and practical, Cardiac Tissue Engineering: Methods and Protocols highlights the major techniques, both experimental and computational, for the study of cardiovascular tissue engineering |
| Analysis |
geneeskunde |
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medicine |
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chemie |
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chemistry |
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biotechnologie |
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biotechnology |
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Chemistry (General) |
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Chemie (algemeen) |
| Bibliography |
Includes bibliographical references and index |
| Notes |
Online resource; title from PDF title page (SpringerLink, viewed August 13, 2014) |
| Subject |
Tissue engineering -- Laboratory manuals
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Myocardium -- Regeneration -- Laboratory manuals
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Heart cells -- Laboratory manuals
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Stem cells -- Laboratory manuals
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Myocardium.
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Myocardium
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Tissue Engineering -- methods
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Tissue engineering
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Myocardium -- Regeneration
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Heart cells
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Stem cells
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Biology -- Laboratory manuals
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| Genre/Form |
Laboratory manuals
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Laboratory manuals.
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Manuels de laboratoire.
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| Form |
Electronic book
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| Author |
Radisic, Milica, editor
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Black, Lauren D., III, editor.
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| ISBN |
9781493910472 |
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1493910477 |
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1493910469 |
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9781493910465 |
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