Title Authentication of Chinese medicinal materials by DNA technology / [edited by] P.C. Shaw, J. Wang, P.P.-H. But

Published River Edge, NJ : World Scientific, ©2002

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Description 1 online resource (xii, 229 pages) : illustrations Contents Molecular markers in Chinese medicinal materials / Pang-Chui Shaw . [and others] -- Methodology and equipment for general molecular techniques / Forrest Chung-Fai Yau and Fai-Ngor Ngan -- Authentication of Chinese medicinal materials by polymerase chain reaction using arbitrarily chosen primers / Hui Cao [and others] -- Authentication of the components from a mixture of herbal materials / Kur-Ta Cheng -- Authentication of Chinese medicinal materials by sequence characterised amplified region / Jun Wang [and others] -- Differentiation of Panax ginseng from P. quinquefolius by direct amplification of length polymorphisms / Wai-Yan Ha [and others] -- Authentication of animal medicinal materials by DNA moecular markers / Yiquan Wang and Kaiya Zhou -- Authentication of medicinal Dendrobium species by the internal transcribed spacer of ribosomal DNA / David Tai-Wai Lau, Pang-Chui Shaw and Paul Pui-Hay But -- Authentication by DNA sequencing of 5s rRNA and trnK genes / Hajime Mizukami -- Authentication based on 18S ribosomal RNA gene and matK gene sequences / Katsuko Komatsu -- Outlook / Pang-Chui Shaw [and others] Summary Traditional Chinese medicine (TCM) has been used for centuries in the Orient to relieve or treat diseases. Its efficacy is now attracting worldwide attention. Chinese medicinal materials are traditionally identified by their organoleptic characteristics, such as the texture or the odour. To ensure consistency of quality and avoid misuse, reliable programs are urgently needed for authentication. Proper authentication also assists in the detection and conservation of endangered species. DNA molecules offer a more definitive means of authentication, as genetic composition is unique for each taxon and is less affected by age, physiological conditions and environmental factors. This book introduces and evaluates the molecular techniques that have been developed to authenticate Chinese medicinal materials. The contents of the book are divided into four parts. The first part reviews the current status of molecular authentication of Chinese medicinal materials. The second part describes the equipment and materials required for the work. In the third part, researchers in the field present the principles and step-by-step protocols for a wide spectrum of techniques used in the molecular authentication of TCM. The last part provides an overview of this field Notes "This volume is published in conjunction with the celebration of the 50th Anniversary of Chung Chi College, the Chinese University of Hong Kong." Bibliography Includes bibliographical references and index Notes Print version record Subject Materia medica -- China -- Analysis DNA fingerprinting. Medicine, Chinese -- Formulae, receipts, prescriptions. Genetics -- Technique. Medicine, Chinese. Materia Medica -- analysis Drug Contamination -- prevention & control Drugs, Chinese Herbal -- standards Genetic Techniques Medicine, Chinese Traditional Pharmaceutical Preparations -- standards Species Specificity DNA Fingerprinting HEALTH & FITNESS -- Herbal Medications. Genetics -- Technique DNA fingerprinting Materia medica -- Analysis Medicine, Chinese China Genre/Form Prescriptions, formulae, receipts, etc. Form Electronic book Author Shaw, P.-C. (Pang-Chui) Wang, Jun But, Paul P. H Chinese University of Hong Kong. Chung Chi College ISBN 9789812706591 9812706593 1281908908 9781281908902

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