Title Matrix metalloproteinase protocols / edited by Ian M. Clark

Published Totowa, N.J. : Humana Press, ©2001

Click on the following:
Springer eBooks

Copies

Description 1 online resource (xv, 545 pages) : illustrations (some color) Series Methods in molecular biology ; 151 Methods in molecular biology (Clifton, N.J.) ; v. 151 Contents Part 1 -- MMPs and TIMPs -- an overview of the field -- Part II -- Expression and purification of MMPs and TIMPs -- Detection of MMPs and TIMPs -- Assay of MMP and TIMP activities Mmps and timps. An historical perspective / J.F. Woessner, Jr. -- Strategies for cloning new mmps and timps / G. Velasco and C. Lopez-Otin -- Structural studies on mmps and timps / W. Bode and K. Maskos -- Matrix metalloproteinase substrate binding domains, modules and exosites. Overview and experimental strategies / C.M. Overall -- The matrix metalloproteinase (mmp) and tissue inhibitor of metalloproteinase (timp) genes. Transcriptional and posttranscriptional regulation, signal transduction and cell-type-specific expression / M.P. Vincenti -- Models for gain-of-function and loss-of-function of mmps. Transgenic and gene targeted mice / L.M. Coussens [and others] -- Expression of mmps and timps in mammalian cells / K.M. Yeow [and others] -- Expression of recombinant matrix metalloproteinases in escherichia coli / L.J. Windsor and D.L. Steele -- Expression of human collagenase i (mmp-1) and timp-1 in a baculovirus-based expression system / R. Vallon and P. Angel -- Expression of recombinant matrix metalloproteinases in yeast / G.A. Doyle -- Expression of recombinant membrane-type mmps / M.J. Butler, M.P. d'Ortho and S.J. Atkinson -- Refolding of timp-2 from escherichia coli inclusion bodies / R.A. Williamson -- Expression and refolding of full-length human timp-1 / D. Davis -- Purification of mmps and timps / K. Shimokawa and H. Nagase -- Monitoring mmp and timp mrna expression by rt-pcr / H. Wong [and others] -- Measuring transcription of metalloproteinase genes. Nuclear run-off assay vs analysis of hnrna / A.M. Delany -- In situ hybridization for metalloproteinases and their inhibitors / T.L. Hurskainen and S.S. Apte -- Use of eia to measure mmps and timps / N. Fujimoto and K. Iwata -- Immunohistochemistry of mmps and timps / Y. Okada -- Detecting polymorphisms in mmp genes / S. Ye and A.M. Henney -- Methods for studying activation of matrix metalloproteinases / V. Knauper and G. Murphy -- Assay of matrix metalloproteinases against matrix substrates / T.E. Cawston, P. Koshy and A.D. Rowan -- Zymography and reverse zymography for detecting mmps, and timps / S.P. Hawkes, H. Li and G.T. Taniguchi -- In situ zymography / S.J. George and J.L. Johnson -- Detection of focal proteolysis using texas-red-gelatin / R.M. Hembry -- Antibodies to mmp-cleaved aggrecan / A.J. Fosang [and others] -- Cartilage proteoglycan release assay / C.J. Billington -- Immunoassay for collagenase-mediated cleavage of types i and ii collagens / R.C. Billinghurst, M. Ionescu and A.R. Poole -- Collagen degradation assays / A.P. Hollander -- Invasion assays and matrix metalloproteinases. Quantification of cellular invasion using propidium iodide labeling and confocal laser scanning microscopy / U. Benbow, K.A. Orndorff and A.L. Givan -- Using fluorogenic peptide substrates to assay matrix metalloproteinases / G.B. Fields -- Kinetic analysis of the inhibition of matrix metalloproteinases by tissue inhibitor of metalloproteinases (timp) / M. Hutton and F. Willenbrock -- Assaying growth factor activity of tissue inhibitors of metalloproteinases / K. Yamashita and T. Hayakawa Summary From the simple discovery in 1962 that resorbing tadpole tail expressed an enzyme (MMP) that could degrade collagen gels, matrix metalloproteinase (MMP) research has advanced to discover more than twenty distinct vertebrate MMPs and four specific inhibitors (TIMPS), a veritable family of enzymes involved in many physiological and pathological processes. In Matrix Metalloproteinase Protocols, leading experts detail proven laboratory techniques for the study of MMPs. The methods include those for the expression and purification of MMPs and TIMPs, for the detection of MMPs and TIMPs at both the protein and mRNA levels, and for the assay of MMP and TIMP activities in a wide variety of circumstances. Each method includes step-by-step instructions and notes on variant applications and pitfalls to avoid. A selective overview of the MMP arena spells out where the field has been, where it is, and where it is going. Comprehensive and highly practical, Matrix Metalloproteinase Protocols brings together the long and hard-earned experience of master experimentalists that will allow not only novices to get up to speed quickly, but also add to the repertoire of successful techniques in expert laboratories Bibliography Includes bibliographical references and index Notes Master and use copy. Digital master created according to Benchmark for Faithful Digital Reproductions of Monographs and Serials, Version 1. Digital Library Federation, December 2002. http://purl.oclc.org/DLF/benchrepro0212 MiAaHDL English Print version record digitized 2010 HathiTrust Digital Library committed to preserve pda MiAaHDL Subject Metalloproteinases -- Laboratory manuals Extracellular matrix proteins -- Laboratory manuals Matrix Metalloproteinases Extracellular Matrix Proteins SCIENCE -- Life Sciences -- Biochemistry. Extracellular matrix proteins Metalloproteinases Metalloproteinasen Grundsubstanz Molekularbiologie Methode Metalloproteïnases. Extracellulaire matrix. Laboratoriumonderzoek. Metaloproteinases. Genre/Form Handbooks and manuals Laboratory manuals Laboratory manuals. Manuels de laboratoire. Form Electronic book Author Clark, Ian M. ISBN 0896037339 9780896037335 9781592590469 1592590462 1592591450 9781592591459 1280820381 9781280820380 9786610820382 6610820384

  Permalink