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Title Microbial proteomics : methods and protocols / edited by Dörte Becher
Published New York : Humana Press : Springer, [2018]
©2018

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Description 1 online resource (xiii, 344 pages) : illustrations (some color)
Series Methods in molecular biology, 1940-6029 ; volume 1841
Methods in molecular biology (Clifton, N.J.) ; v. 1841. 1064-3745
Contents Filter-aided sample preparation for proteome analysis / Jacek R. Wisniewski -- Protein enrichment from highly dilute samples with StrataClean / Florian Bonn and Andreas Otto -- Membrane proteomics in gram-positive bacteria : two complementary approaches to target the hydrophobic species of proteins / Susanne Sievers -- Enrichment of cell surface-associated proteins in gram-positive bacteria by biotinylation or trypsin shaving for mass spectrometry analysis / Florian Bonn, Sandra Maass, and Jan Maarten van Dijl -- Preparation of bacterial magnetosomes for proteome analysis / Oliver Raschdorf, Dirk Schuler, and Rene Uebe -- Analysis of Legionella metabolism by pathogen vacuole proteomics / Christian Manske, Ivo Finsel, Christine Hoffmann, and Hubert Hilbi -- Detection and identification of low-abundant proteins using HPE gels, fluorescent stains, and MALDI-ToF-ToF-MS / Martin Moche, Dirk Albrecht, Reiner Westermeier, and Knut Buttner -- Applications of difference gel electrophoresis (DIGE) in the study of microorganisms / Kathleen Trautwein and Ralf Rabus -- Proteomic signatures in Staphylococcus aureus / Susanne Engelmann and Stephan Fuchs -- How to assess protein stability : half-life determination of a regulatory protein in Bacillus subtilis / Alexander Reder, Stephan Michalik, and Ulf Gerth -- Absolute protein quantification using AQUA-calibrated 2D-PAGE / Sandra Maass -- Sulfur-34S and 36S stable isotope labeling of amino acids for quantification (SULAQ34/36) of proteome analyses / Florian-Alexander Herbst, Nico Jehmlich, Martin von Bergen, and Frank Schmidt -- Metabolic labeling of microorganisms with stable heavy nitrogen isotopes (15N) / Andreas Otto -- Next-generation trapping of protease substrates by label-free proteomics / Claudia Lindemann, Nikolas Thomanek, Katja Kuhlmann, Helmut E. Meyer, Katrin Marcus, and Franz Narberhaus -- In vivo proteomics approaches for the analysis of bacterial adaptation reactions in host-pathogen settings / Henrike Pfortner, Maren Depke, Kristin Surmann, Frank Schmidt, and Uwe Volker -- Phosphopeptide enrichment from bacterial samples utilizing titanium oxide affinity chromatography / Boumediene Soufi, Christoph T€aumer, Maja Semanjski, and Boris Macek -- Phosphoproteomics in microbiology : protocols for studying Streptomyces coelicolor differentiation / Angel Manteca, Beatriz Rioseras, Adelina Rogowska-Wrzesinska, and Ole N. Jensen -- Thiol-redox proteomics to study reversible protein thiol oxidations in bacteria / Martina Rossius, Falko Hochgr€afe, and Haike Antelmann -- Sequential isolation of DNA, RNA, protein, and metabolite fractions from murine organs and intestinal contents for integrated omics of host-microbiota interactions / Pranjul Shah, Emilie E.L. Muller, Laura A. Lebrun, Linda Wampach, and Paul Wilmes -- Utilization of a detergent-based method for direct microbial cellular lysis/proteome extraction from soil samples for metaproteomics studies / Karuna Chourey and Robert L. Hettich -- Sample preparation for metaproteome analyses of soil and leaf litter / Katharina M. Keiblinger and Katharina Riedel -- Centrifugation-based enrichment of bacterial cell populations for metaproteomic studies on bacteria-invertebrate symbioses / Tjorven Hinzke, Manuel Kleiner, and Stephanie Markert
Bibliography Includes bibliographical references and index
Notes Online resource; title from PDF title page (SpringerLink, viewed September 26, 2018)
Subject Microbial proteins -- Laboratory manuals
Proteomics -- Laboratory manuals
Staphylococcus aureus.
Bacillus subtilis.
Proteomics.
Legionella.
Bacteria -- chemistry
Gram-Positive Bacteria -- isolation & purification
Staphylococcus aureus
Streptomyces coelicolor
Bacillus subtilis
Proteomics
Legionella
Proteins.
Science -- Life Sciences -- Biochemistry.
Staphylococcus aureus
Legionella
Bacillus subtilis
Microbial proteins
Proteomics
Genre/Form Laboratory manuals
Laboratory manuals.
Manuels de laboratoire.
Form Electronic book
Author Becher, Dörte, editor
ISBN 9781493986958
1493986953
1493986937
9781493986934