| Description |
1 online resource (xvi, 439 pages) : illustrations (some color) |
| Series |
Methods in molecular biology, 1064-3745 ; 1321 |
|
Methods in molecular biology (Clifton, N.J.) ; 1321. 1064-3745
|
| Contents |
880-01 Current approaches to engineering N-linked protein glycosylation in bacteria / Andreas Naegeli and Markus Aebi -- Inverse metabolic engineering for enhanced glycoprotein production in Escherichia coli / Stephen R.P. Jaffé, Benjamin Strutton, Jagroop Pandhal, and Phillip C. Wright -- GlycoSNAP : a high-throughput screening methodology for engineering designer glycosylation enzymes / Anne A. Ollis, Yi Chai, and Matthew P. DeLisa -- Production of glycoproteins with asparagine-linked N-acetylglucosamine in Escherichia coli / Flavio Schwarz and Markus Aebi -- Glyco-engineering O-antigen-based vaccines and eiagnostics in E. coli / Nicolas F. Vozza and Mario F. Feldman -- Progress in yeast glycosylation engineering / Stephen R. Hamilton and Dongxing Zha -- Protein production with a Pichia pastoris OCH1 knockout strain in fed-batch mode / Christoph Gmeiner and Oliver Spadiut -- Engineering the Pichia pastoris N-glycosylation pathway using the GlycoSwitch technology / Bram Laukens, Charlot De Wachter, and Nico Callewaert -- Development of a valuable yeast strain using a novel mutagenesis technique for the effective production of therapeutic glycoproteins / Hiroko Abe and Ken-ichi Nakayama -- An overview and history of glyco-engineering in insect expression systems / Christoph Geisler, Hideaki Mabashi-Asazuma, and Donald L. Jarvis 11 SweetBac : applying multiBac technology towards flexible modification of insect cell glycosylation / Dieter Palmberger and Dubravko Rendic -- Engineering sialic acid synthesis ability in insect cells / Karthik Viswanathan, Someet Narang, and Michael J. Betenbaugh -- Engineering N-glycosylation pathway in insect cells : suppression of [beta]-N-acetylglucosaminidase and expression of [beta]-1,4-galactosyltransferase / Yeon Kyu Kim and Hyung Joon Cha -- N-glyco-engineering in plants : update on strategies and major achievements / Herta Steinkellner and Alexandra Castilho -- Gene targeting for precision glyco-engineering : production of biopharmaceuticals devoid of plant-typical glycosylation in moss bioreactors / Eva L. Decker, Gertrud Wiedemann, and Ralf Reski -- Im"plant"ing of mammalian glycosyltransferase gene into plant suspension-cultured cells using agrobacterium-mediated transformation / Hiroyuki Kajiura and Kazuhito Fujiyama -- Transient glyco-engineering of N. benthamiana aiming at the synthesis of multi-antennary sialylated proteins / Andreas Loos and Alexandra Castilho -- Subcellular targeting of proteins involved in modification of plant N- and O-glycosylation / Martina Dicker, Jennifer Schoberer, Ulrike Vavra, and Richard Strasser -- Assembly of multigene constructs using golden gate cloning / Sylvestre Marillonnet and Stefan Werner -- Strategies for engineering protein N-glycosylation pathways in mammalian cells / Qiong Wang, Mark Stuczynski, Yuan Gao, and Michael J. Betenbaugh -- Glycan remodeling with processing inhibitors and lectin- resistant eukaryotic cells / Veronica T. Chang, Robert A. Spooner, Max Crispin, and Simon J. Davis -- Production of highly sialylated recombinant glycoproteins using Ricinus communis agglutinin-I-resistant CHO glycosylation mutants / John S.Y. Goh, Kah Fai Chan, and Zhiwei Song -- Metabolic glyco-engineering in eukaryotic cells and selected applications / Friedrich Piller, Aline Mongis, and Véronique Piller -- Evaluation of quenching and extraction methods for nucleotide/nucleotide sugar analysis / Katrin Braasch, Carina Villacrés, and Michael Butler -- Chemoenzymatic glyco-engineering of monoclonal antibodies / John P. Giddens and Lai-Xi Wang -- Chemical polysialylation of recombinant human proteins / Ivan V. Smirnov, Ivan I. Vorobiev, Alexey A. Belogurov, Dmitry D. Genkin, Sergey M. Deyev, and Alexander G. Gabibov -- Site-specific glycosylation profiling using liquid chromatography-tandem mass spectrometry (LC-MS) / Clemens Gruber and Friedrich Altmann -- Mass spectrometric analysis of oligo- and polysialic acids / Christina E. Galuska, Kai Maass, and Sebastian P. Galuska -- Isomer-specific analysis of released N-glycans by LC-ESI MS/MS with porous graphitized carbon / Daniel Kolarich, Markus Windwarder, Kathirvel Alagesan, and Friedrich Altmann |
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880-01/(S Current approaches to engineering N-linked protein glycosylation in bacteria / Andreas Naegeli and Markus Aebi -- Inverse metabolic engineering for enhanced glycoprotein production in Escherichia coli / Stephen R.P. Jaffé, Benjamin Strutton, Jagroop Pandhal, and Phillip C. Wright -- GlycoSNAP : a high-throughput screening methodology for engineering designer glycosylation enzymes / Anne A. Ollis, Yi Chai, and Matthew P. DeLisa -- Production of glycoproteins with asparagine-linked N-acetylglucosamine in Escherichia coli / Flavio Schwarz and Markus Aebi -- Glyco-engineering O-antigen-based vaccines and eiagnostics in E. coli / Nicolas F. Vozza and Mario F. Feldman -- Progress in yeast glycosylation engineering / Stephen R. Hamilton and Dongxing Zha -- Protein production with a Pichia pastoris OCH1 knockout strain in fed-batch mode / Christoph Gmeiner and Oliver Spadiut -- Engineering the Pichia pastoris N-Glycosylation Pathway Using the GlycoSwitch Technology / Bram Laukens, Charlot De Wachter, and Nico Callewaert -- Development of a valuable yeast strain using a novel mutagenesis technique for the effective production of therapeutic glycoproteins / Hiroko Abe and Ken-ichi Nakayama -- Overview and history of glyco-engineering in insect expression systems / Christoph Geisler, Hideaki Mabashi-Asazuma, and Donald L. Jarvis 11 SweetBac : applying multiBac technology towards flexible modification of insect cell glycosylation / Dieter Palmberger and Dubravko Rendic -- Engineering sialic acid synthesis ability in insect cells / Karthik Viswanathan, Someet Narang, and Michael J. Betenbaugh -- Engineering N-glycosylation pathway in insect cells : suppression of β-N-acetylglucosaminidase and expression of β-1,4-galactosyltransferase / Yeon Kyu Kim and Hyung Joon Cha -- N-glyco-engineering in plants : update on strategies and major achievements / Herta Steinkellner and Alexandra Castilho -- Gene targeting for precision glyco-engineering : production of biopharmaceuticals devoid of plant-typical glycosylation in moss bioreactors / Eva L. Decker, Gertrud Wiedemann, and Ralf Reski -- Im"plant"ing of mammalian glycosyltransferase gene into plant suspension-cultured cells using agrobacterium- mediated transformation / Hiroyuki Kajiura and Kazuhito Fujiyama -- Transient glyco-engineering of N. benthamiana aiming at the synthesis of multi-antennary sialylated proteins / Andreas Loos and Alexandra Castilho -- Subcellular targeting of proteins involved in modification of plant N- and O-glycosylation / Martina Dicker, Jennifer Schoberer, Ulrike Vavra, and Richard Strasser -- Assembly of multigene constructs using golden gate cloning / Sylvestre Marillonnet and Stefan Werner -- Strategies for engineering protein N-glycosylation pathways in mammalian cells / Qiong Wang, Mark Stuczynski, Yuan Gao, and Michael J. Betenbaugh -- Glycan remodeling with processing inhibitors and lectin- resistant eukaryotic cells / Veronica T. Chang, Robert A. Spooner, Max Crispin, and Simon J. Davis -- Production of highly sialylated recombinant glycoproteins using Ricinus communis agglutinin-I-resistant CHO glycosylation mutants / John S.Y. Goh, Kah Fai Chan, and Zhiwei Song -- Metabolic glyco-engineering in eukaryotic cells and selected applications / Friedrich Piller, Aline Mongis, and Véronique Piller -- Evaluation of quenching and extraction methods for nucleotide/nucleotide sugar analysis / Katrin Braasch, Carina Villacrés, and Michael Butler -- Chemoenzymatic glyco-engineering of monoclonal antibodies / John P. Giddens and Lai-Xi Wang -- Chemical polysialylation of recombinant human proteins / Ivan V. Smirnov, Ivan I. Vorobiev, Alexey A. Belogurov, Dmitry D. Genkin, Sergey M. Deyev, and Alexander G. Gabibov -- Site-specific glycosylation profiling using liquid chromatography-tandem mass spectrometry (LC-MS) / Clemens Gruber and Friedrich Altmann -- Mass spectrometric analysis of oligo- and polysialic acids / Christina E. Galuska, Kai Maass, and Sebastian P. Galuska -- Isomer-specific analysis of released N-glycans by LC-ESI MS/MS with porous graphitized carbon / Daniel Kolarich, Markus Windwarder, Kathirvel Alagesan, and Friedrich Altmann |
| Summary |
Annotation Conceived with the intention of providing an array of strategies and technologies currently in use for glyco-engineering distinct living organisms, this book contains a wide range of methods being developed to control the composition of carbohydrates and the properties of proteins through manipulations on the production host rather than in the protein itself. The first five sections deal with host-specific glyco-engineering and contain chapters that provide protocols for modifications of the glycosylation pathway in bacteria, yeast, insect, plants and mammalian cells, while the last two sections explore alternative approaches to host glyco-engineering and selected protocols for the analysis of the N-glycans and glyco-profiling by mass spectrometry. Written for the highly successful Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols and tips on troubleshooting and avoiding known pitfalls. Authoritative and extensive, Glyco-Engineering: Methods and Protocols offers vast options to help researchers to choose the expression system and approach that best suits their intended protein research or applications |
| Bibliography |
Includes bibliographical references and index |
| Notes |
Online resource; title from PDF title page (SpringerLink, viewed June 26, 2015) |
| Subject |
Glycosylation -- Laboratory manuals
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Glycosylation.
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Glycoproteins.
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Glycosylation
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Glycoproteins
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Proteins.
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Science -- Life Sciences -- Biochemistry.
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Glycoproteins
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Glycosylation
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| Genre/Form |
Laboratory manuals
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Laboratory manuals.
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Manuels de laboratoire.
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| Form |
Electronic book
|
| Author |
Castilho, Alexandra, editor.
|
| ISBN |
9781493927609 |
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1493927604 |
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1493927590 |
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9781493927593 |
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