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Title Glycoprotein analysis in biomedicine / edited by Elizabeth F. Hounsell
Published Totowa, N.J. : Humana Press, ©1993

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Description 1 online resource (xi, 306 pages) : illustrations
Series Methods in molecular biology ; v. 14
Methods in molecular biology (Clifton, N.J.) ; v. 14
Contents General strategy for glycoprotein oligosaccharide analysis / Elizabeth F. Hounsell -- Analysis of asparagine-linked oligosaccharides by sequential lectin-affinity chromatography / Kazuo Yamamoto, Tsutomu Tsuji and Toshiaki Osawa -- Sequence and linkage analysis of N- and O-linked glycans by fast atom bombardment mass spectrometry / Bo Nilsson -- Gas chromatography and gas chromatography-mass spectrometry of glycoprotein oligosaccharides / Gunnar C. Hansson and Hasse Karlsson -- Microscale sequencing of N-linked oligosaccharides of glycoproteins using hydrazinolysis, bio-gel P-4, and sequential exoglycosidase digestion / Tsuguo Mizuochi -- Analysis of sugar chains by pyridylamination / Sumihiro Hase -- Analysis of carbohydrates in glycoproteins by high-performance liquid chromatography and high-performance capillary electrophoresis / Kazuaki Kakehi and Susumu Honda -- Microanalysis of glyocosyl-phosphatidylinositol glycans / M. Lucia Guther and Michael A.J. Ferguson -- Analysis of bacterial glycoproteins / Johann Lechner and Felix Wieland -- Isolation and fractionation of glycoprotein glycans in small amounts / Rudolf Geyer and Hildegard Geyer -- High-mannose chains of mammalian glycoproteins / Anne P. Sherblom and Rosalita M. Smagula -- Release of O-linked glycoprotein glycans by endo-[alpha]-N-acetylgalactosaminidase / Hitoo Iwase and Kyoko Hotta -- Immunological detection of glycoproteins on blots based on labeling with digoxigenin / Anton Haselbeck and Wolfgang Hosel -- Glycosyltransferases as tools in cell biological studies / Robert S. Haltiwanger and Gerald W. Hart -- Purification of the EGF receptor for oligosaccharide studies / Mary Gregoriou -- Analysis of sulfated polysaccharide conjugates / Christopher C. Rider -- Secreted mucus glycoproteins in cell and organ culture / Anthony P. Corfield and Christos Paraskeva -- Preparation of polyclonal antibodies to native and modified mucin antigens / Ismat Khatri, Gordon Forstner and Janet Forstner -- Use of immunoperoxidase to characterize gastrointestinal mucin epitopes / Jacques Bara and Rafael Oriol -- Application of lectins to the study of mucosal glycoproteins / Jonathan M. Rhodes and Chi Kong Ching -- Detection of tumor-associated expression of carbohydrate-binding proteins (lectins): the use of neoglycoproteins and neoglycoenzymes in glycohistochemical and glycocytological studies / Hans-Joachim Gabius and Sigrun Gabius -- Scanning tunneling microscopy of biopolymers / Terence J. McMaster and Victor J. Morris
Summary Glycoprotein Analysis in Biomedicine brings together a comprehensive range of protocols related to carbohydrate biochemistry. The first half of the manual outlines physiochemical (MS and NMR), chemical, and chromatographic techniques for defining the structures and diversity of oligosaccharide sequences. The second half describes more biological and immunological approaches used to detect changes in glycosylation patterns in disease
Analysis Glycoproteins Analysis
Bibliography Includes bibliographical references and index
Notes English
Print version record
Subject Glycoproteins -- Analysis
Glycoproteins -- analysis
Glycoproteins -- Analysis
Glycoprotéines.
Biologie moléculaire -- Méthodes.
Chemische Analyse
Glykoproteine
Glycoproteïnen.
Chemische analyses.
Wetenschappelijke technieken.
Glycoprotéines -- Analyse.
Genre/Form Laboratory manuals
Laboratory manuals.
Manuels de laboratoire.
Form Electronic book
Author Hounsell, Elizabeth F.
ISBN 9781592595013
1592595014