| Description |
1 online resource (xviii, 381 pages) : illustrations |
| Series |
Methods in molecular biology, 1064-3745 ; 347 |
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Methods in molecular biology (Clifton, N.J.) ; v. 347
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| Contents |
Application of fluorophore-assisted carbohydrate electrophoresis for the study of the dolichol pyrophosphate-linked oligosaccharides pathway in cell cultures and animal tissues / Ningguo Gao -- Partial purification of mannosylphosphorylundecaprenol synthase from Micrococcus luteus: a useful enzyme for the biosynthesis of a variety of mannosylphosphorylpolyisoprenol products / Jeffrey S. Rush and Charles J. Waechter -- Assay of dolichyl-phospho-mannose synthase reconstituted in a lipid matrix / John S. Schutzbach -- O-mannosylation in mammalian cells / Tamao Endo and Hiroshi Manya -- Methods for analysis of unusual forms of o-glycosylation / Aleksandra Nita-Lazar and Robert S. Haltiwanger -- Structural analysis of naturally occurring sialic acids / Johannis P. Kamerling and Gerrit J. Gerwig |
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Nonradioactive trans-sialidase screening assay / Silke Schrader and Roland Schauer -- Structural determination of o-glycans by tandem mass spectrometry / Catherine Robbe, Jean-Claude Michalski, and Calliope Capon -- Detailed structural analysis of n-glycans released from glycoproteins in SDS-PAGE gel bands using HPLC combined with exoglycosidase array digestions / Louise Royle [and others] -- Molecular modeling of glycosyltransferases / Anne Imberty [and others] -- [beta]-Galactoside [alpha]2,6-sialyltransferase and the sialyl [alpha]2,6-galactosyl-linkage in tissues and cell lines / Fabio Dall'Olio, Nadia Malagolini, and Mariella Chiricolo -- Visualizing intracellular distribution and activity of core2 [beta](1,6)n-acetylglucosaminyltransferase-1 in living cells / Maëlle Prorok-Hamon, Sylvie Mathieu, and Assou El-Battari |
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Gene expression analysis of glycosylation-related genes by real-time polymerase chain reaction / Juan J. Garcia-Vallejo [and others] -- Analysis of the glycodynamics of primary osteoblasts and bone cancer cells / Inka Brockhausen, Xiaojing Yang, and Mark Harrison -- Micromethods for the characterization of lipid A-core and o-antigen lipopolysaccharide / Cristina L. Marolda [and others] -- Assays for a galactosyltransferase involved in the assembly of the O7-antigen repeat unit of escherichia coli / Inka Brockhausen [and others] -- High-throughput quantitation of metabolically labeled anionic glycoconjugates by scintillation proximity assay utilizing binding to cationic dyes / Karen J. Rees-Milton and Tassos P. Anastassiades -- Quantitative analysis of mucins in mucosal secretions using indirect enzyme-linked immunosorbent assay / Pablo Argueso and Ilene K. Gipson -- Molecular dissection of the mouse zona pellucida: an electron-microscopic perspective utilizing high-resolution colloidal-gold labeling methods / Frederick W.K. Kan |
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Soluble adamantly glycosphingolipid analogs as probes of glycosphingolipid function / Clifford Lingwood [and others] -- Diagnosis of Krabbe disease by use of a natural substrate / John W. Callahan and Marie-Anne Skomorowski -- In vitro assays of the functions of calnexin and calreticulin, lectin chaperones of the endoplasmic reticulum / Breanna S. Ireland, Monika Niggemann, and David B. Williams -- Quantitative measurement of selectin-ligand interactions: assays to identify a sweet pill in a library of carbohydrates / Mark E. Beauharnois, Sriram Neelamegham, and Khushi L. Matta -- Sialic acid-binding specificities of siglecs / Nadine Bock and Sørge Kelm |
| Summary |
Glycobiology involves studies of complex carbohydrates and posttrans- tional modifications of proteins, and has become an important interdiscip- nary field encompassing chemistry, biochemistry, biology, physiology, and pathology. Although initial research was directed toward elucidation of the different carbohydrate structures and the enzymes synthesizing them, the field has now moved toward identifying the functions of carbohydrates. The pro- cols described in Glycobiology Protocols form a solid basis for investigations of glycan functions in health and disease. The cloning of many of the genes participating in glycosylation processes has helped to enhance our knowledge of how glycosylation is controlled, but has also added another dimension of complexity to the great heterogeneous variety of the structures of the oligos- charides of glycoproteins, proteoglycans, and glycolipids. A family of similar enzyme proteins exists for each glycosylation step. Glycosyltransferases are extremely specific for both the nucleotide sugar donor and the acceptor s- strate, but many other factors control sugar transfer, including the locali- tion and topology of enzymes, cofactors, possible chaperone proteins, and the availability of sugar acceptor substrates. The analysis of the intracellular organization of glycosylation and of the factors controlling the activities of the participating enzymes in the cell are important areas that need more research efforts. Another challenge for future research is to understand the glycodynamics of a cell, that is, how the cell responds to stimuli leading to biological and pathological changes in terms of alterations in glycosylation, and how this affects the biology of the cell |
| Analysis |
glycobiologie |
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glycobiology |
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Molecular Biology (General) |
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Moleculaire biologie (algemeen) |
| Bibliography |
Includes bibliographical references and index |
| Notes |
Print version record |
| Subject |
Glycoconjugates.
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Glycoconjugates -- Physiological effect
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Glycoproteins -- physiology
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Glycoconjugates
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Molecular Biology -- methods
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SCIENCE -- Life Sciences -- Biochemistry.
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Glycoconjugates.
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Glycoconjugates -- Physiological effect.
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Glycoproteins -- physiology.
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Molecular biology -- Methods.
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Glycoconjugates
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| Genre/Form |
Laboratory manuals
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Laboratory manuals.
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Manuels de laboratoire.
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| Form |
Electronic book
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| Author |
Brockhausen, Inka, 1944-
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| ISBN |
9781597451673 |
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1597451673 |
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