| Description |
1 online resource (xi, 274 pages) : illustrations (some color) |
| Series |
Methods in molecular biology, 1940-6029 ; volume 1713 |
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Methods in molecular biology (Clifton, N.J.) ; v. 1713. 1064-3745
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| Contents |
Expression and purification of rat glucose transporter in Pichia pastoris / Raminta Venskutonyte, Karin Elbing, and Karin Lindkvist-Petersson -- Crystallization and structural setermination of the human glucose transporters GLUT1 and GLUT3 / Dong Deng and Nieng Yan -- Screening and scale-up of GLUT transporter constructs suitable for biochemical and structural studies / Gregory Verdon, Hae Joo Kang, and David Drew -- GLUT characterization using frog Xenopus laevis oocytes / Wentong Long, Debbie O'Neill, and Chris I. Cheeseman -- Glucose uptake in heterologous expression systems / Eunice E. Lee and Richard C. Wang -- Evaluating the efficacy of GLUT inhibitors using a seahorse extracellular flux analyzer / Changyong Wei, Monique Heitmeier, Paul W. Hruz, and Mala Shanmugam -- Glucose transport activity measured in giant vesicles / Jesper S. Hansen and Karin Lindkvist-Petersson -- Design, synthesis, and evaluation of GLUT inhibitors / Carlotta Granchi, Tiziano Tuccinardi, and Filippo Minutolo-- Applying microfluidic systems to study effects of glucose at single-cell level / Niek Welkenhuysen, Caroline B. Adiels, Mattias Goksor, and Stefan Hohmann -- Growth-based screening system for hexose transporters in yeast / Eckhard Boles and Mislav Oreb -- Identification of insulin-activated Rab proteins in adipose cells using Bio-ATB-GTP photolabeling technique / Francoise Koumanov and Geoffrey D. Holman -- Total internal reflection fluorescence microscopy to study GLUT4 trafficking / Sebastian Wasserstrom, Björn Morén, and Karin G. Stenkula -- Translocation and redistribution of GLUT4 using a dual-labeled reporter assay / Robert M. Jackson and Ann Louise Olson -- GLUT4 translocation in single muscle cells in culture : epitope detection by immunofluorescence / Javier R. Jaldin-Fincati, Philip J. Bilan, and Amira Klip -- Glucose transport : methods for interrogating GLUT4 trafficking in adipocytes / Dougall M. Norris, Tom A. Geddes, David E. James, Daniel J. Fazakerley, and James G. Burchfield -- Proximity ligation assay to study the GLUT4 membrane trafficking machinery / Dimitrios Kioumourtzoglou, Gwyn W. Gould, and Nia J. Bryant -- Quantification of cell-surface glucose transporters in the heart using a biotinylated photolabeling assay / Zahra Maria and Véronique A. Lacombe -- Tracking GLUT2 translocation by live-cell imaging / Sabina Tsytkin-Kirschenzweig, Merav Cohen, and Yaakov Nahmias -- GLUT2-expressing neurons as glucose sensors in the brain : electrophysiological analysis / Gwenaël Labouèbe, Bernard Thorens, and Christophe Lamy |
| Summary |
This volume explores methods used to examine the structure-function relationship of glucose transporters both in vitro and ex vivo. Chapters in this book cover topics such as expression and purification of glucose transporters in heterologous expression systems, crystal structure determination, glucose transport activity assays in cellular and synthetic model systems, investigation of the GLUT4 membrane trafficking machinery by novel labeling strategies combined with advanced fluorescence microscopy methods, and tracking GLUT2 translocation by live cell imaging. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Cutting-edge and comprehensive, Glucose Transport: Methods and Protocols is a valuable resource that helps researchers with determining specific roles of different GLUTs in various organisms |
| Bibliography |
Includes bibliographical references and index |
| Notes |
Online resource; title from PDF title page (SpringerProtocol, viewed December 11, 2017) |
| Subject |
Glucose.
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Glucose
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Glucose Transport Proteins, Facilitative
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Cellular biology (cytology)
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Science -- Life Sciences -- Biology -- Molecular Biology.
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Glucose
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| Genre/Form |
Laboratory manuals
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Laboratory manuals.
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Manuels de laboratoire.
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| Form |
Electronic book
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| Author |
Hansen, Jesper S., editor
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Lindkvist-Petersson, Karin, editor
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| ISBN |
9781493975075 |
|
1493975072 |
|
1493975064 |
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9781493975068 |
|
