| Description |
xi, 276 pages : illustrations (black and white, and colour) ; 27 cm |
| Series |
Methods in molecular biology, 1064-3745 ; 1448 |
|
Methods in molecular biology. 1064-3745 ; 1448
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| Contents |
Contents note continued: 7.Production and Concentration of Lentivirus for Transduction of Primary Human T Cells / Adam P. Cribbs -- 8.Generating Transgenic Mice by Lentiviral Transduction of Spermatozoa Followed by In Vitro Fertilization and Embryo Transfer / Robert Winston -- 9.The LAM-PCR Method to Sequence LV Integration Sites / Manfred Schmidt -- 10.Conditional RNAi Using the Lentiviral GLTR System / Stephan Geley -- 11.Lentiviral Vectors for the Engineering of Implantable Cells Secreting Recombinant Antibodies / Bernard L. Schneider -- pt. III Integrase-Mutant LVs -- 12.Transient Expression of Green Fluorescent Protein in Integrase-Defective Lentiviral Vector-Transduced 293T Cell Line / A. Hamid -- 13.Intrastriatal Delivery of Integration-Deficient Lentiviral Vectors in a Rat Model of Parkinson's Disease / Rafael J. Yanez-Munoz -- 14.Development of Lentiviral Vectors for Targeted Integration and Protein Delivery / Seppo Yla-Herttuala -- |
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Contents note continued: pt. IV Production, Detection, and Engineering of Exosomes -- 15.Biogenesis and Functions of Exosomes and Extracellular Vesicles / Andreas Baur -- 16.Generation, Quantification, and Tracing of Metabolically Labeled Fluorescent Exosomes / Massimo Sargiacomo -- 17.Cardiac Myocyte Exosome Isolation / Anne A. Knowlton -- 18.Incorporation of Heterologous Proteins in Engineered Exosomes / Maurizio Federico -- 19.Exosome-Mediated Targeted Delivery of miRNAs / Masahiko Kuroda |
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Machine generated contents note: pt. I Recent Improvements in LV Construction, Production, and Transduction -- 1.Construction of Modular Lentiviral Vectors for Effective Gene Expression and Knockdown / David Dankort -- 2.Development of Inducible Molecular Switches Based on All-in-One Lentiviral Vectors Equipped with Drug Controlled FLP Recombinase / Axel Schambach -- 3.Production of Retrovirus-Based Vectors in Mildly Acidic pH Conditions / David Fenard -- 4.Optimized Lentiviral Transduction Protocols by Use of a Poloxamer Enhancer, Spinoculation, and scFv-Antibody Fusions to VSV-G / Christian Thirion -- pt. II New LV Targets and Applications -- 5.Transduction of Murine Hematopoietic Stem Cells with Tetracycline-regulated Lentiviral Vectors / Olga S. Kustikova -- 6.Introduction of shRNAs, miRNAs, or AntagomiRs into Primary Human Liver Cells Through Lentiviral Vectors / Maria Thomas -- |
| Bibliography |
Includes bibliographical references and index |
| Notes |
Also published electronically |
| Subject |
Genetic vectors.
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Lentiviruses -- Laboratory manuals.
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Lentiviruses.
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Lentivirus -- genetics.
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Cell Engineering -- methods.
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Exosomes -- genetics.
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Genetic Vectors -- therapeutic use.
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Recombination, Genetic.
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| Genre/Form |
Laboratory manuals.
|
| Author |
Federico, Maurizio, editor
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| LC no. |
2016941747 |
| ISBN |
9781493937516 (hardback) |
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9781493937530 (eBook) |
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