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Genetic engineering
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--subdivision Genetic engineering under individual animals and groups of animals, e.g. Cattle--Genetic engineering; Fishes--Genetic engineering
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Here are entered works on the application of living organisms or their biological systems or processes to the manufacture of useful products --subdivision Biotechnology under individual chemicals and groups of chemicals, e.g. Insulin--Biotechnology
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-- See Also the narrower term Cloning
--subdivision Cloning under individual animals and groups of animals, e.g. Cattle--Cloning; Fishes--Cloning
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Biologically active DNA which has been formed by the in vitro joining of segments of DNA from different sources. It includes the recombination joint or edge of a heteroduplex region where two recombining DNA molecules are connected
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An assisted reproductive technique that includes the direct handling and manipulation of oocytes and sperm to achieve fertilization in vitro
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-- See Also the narrower term Gene therapy
--subdivision Gene therapy under individual diseases and types of diseases, e.g. Cancer--Gene therapy
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Strains of mice in which certain GENES of their GENOMES have been disrupted, or "knocked-out". To produce knockouts, using RECOMBINANT DNA technology, the normal DNA sequence of the gene being studied is altered to prevent synthesis of a normal gene product. Cloned cells in which this DNA alteration is successful are then injected into mouse EMBRYOS to produce chimeric mice. The chimeric mice are then bred to yield a strain in which all the cells of the mouse contain the disrupted gene. Knockout mice are used as EXPERIMENTAL ANIMAL MODELS for diseases (DISEASE MODELS, ANIMAL) and to clarify the functions of the genes
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Laboratory mice that have been produced from a genetically manipulated EGG or EMBRYO, MAMMALIAN
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Organisms whose GENOME has been changed by a GENETIC ENGINEERING technique
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--subdivision Genetic engineering under individual plants and groups of plants, e.g. Corn--Genetic engineering
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PLANTS, or their progeny, whose GENOME has been altered by GENETIC ENGINEERING
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Here are entered works on biochemical and genetic engineering processes involved in the synthesis, modification, and production of protein products for various applications
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Procedures by which protein structure and function are changed or created in vitro by altering existing or synthesizing new structural genes that direct the synthesis of proteins with sought-after properties. Such procedures may include the design of MOLECULAR MODELS of proteins using COMPUTER GRAPHICS or other molecular modeling techniques; site-specific mutagenesis (MUTAGENESIS, SITE-SPECIFIC) of existing genes; and DIRECTED MOLECULAR EVOLUTION techniques to create new genes
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