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Book Cover
E-book

Title Baculovirus and insect cell expression protocols / edited by David W. Murhammer
Edition 2nd ed
Published Totowa, N.J. : Humana Press, ©2007

Copies

Description 1 online resource (xiv, 470 pages) : illustrations (some color)
Series Methods in molecular biology ; 388
Methods in molecular biology (Clifton, N.J.) ; v. 388
Contents Useful tips, widely used techniques, and quantifying cell metabolic behavior / D.W. Murhammer -- Introduction to baculovirus molecular biology / B.J. Kelly [and others] -- Baculovirus transfer vectors / R.D. Possee [and others] -- Recombinant baculovirus isolation / L.A. King [and others] -- Gene expression in mammalian cells using BacMam, a modified baculovirus system / J.A. Fornwald [and others] -- Available lepidopteran insect cell lines / D.E. Lynn -- Lepidopteran insect cell line isolation from insect tissue / D.E. Lynn -- Development of serum-free media for lepidopteran insect cell lines / S.N. Agathos -- Routine maintenance and storage of lepidopteran insect cell lines and baculoviruses / D.E. Lynn -- Small-scale protein production with the baculovirus expression vector system / S. Harwood -- Recombinant protein production in large-scale agitated bioreactors using the baculovirus expression vector system / C.B. Elias [and others] -- Production of baculovirus-expressed recombinant proteins in wave bioreactors / S.H. Kadwell [and others] -- Protein production with recombinant baculoviruses in lepidopteran larvae / Y. Liu [and others] -- Virus-like particle and viral vector production using the baculovirus expression vector system/insect cell system: Adeno-associated virus-based products / M.G. Aucoin [and others] -- Transforming lepidopteran insect cells for continuous recombinant protein expression / R.L. Harrison [and others] -- Transformation of drosophila cell lines: An alternative approach to exogenous protein expression / L. Cherbas [and others] -- Transforming lepidopteran insect cells for improved protein processing / R.L. Harrison [and others] -- Introduction to the use of baculoviruses as biological insecticides / B.C. Bonning [and others] -- Baculovirus insecticide production in insect larvae / N. van Beek [and others] -- Evaluation of the insecticidal efficacy of wild-type and recombinant baculoviruses / H. Li [and others] -- Monitoring and visualization of baculovirus infection using green fluorescent protein strategy / H.J. Cha [and others] -- Alternative bioreactor strategy for probing infection and production / Y.-C. Hu [and others] -- Methods for gene silencing with rnai / J.C. March [and others] -- Use of the baculovirus/insect cell system to study apoptosis / N. Chejanovsky -- Generation of envelope-modified baculoviruses for gene delivery into mammalian cells / C. Hofmann
Summary Baculovirus Expression Protocols is a detailed guide for using the baculovirus expression vector system (BEVS) and/or insect cells to produce recombinant proteins. Procedures for the development and production of baculovirus insecticides at both laboratory and large scale are described in the kind of step-by-step format that the Methods in Molecular Biology series helped standardize. This book is a one-stop source for information on baculoviruses, and includes sections on using modified baculoviruses to express genes in mammalian cells, using Drosophila cell lines, isolating new cell lines, and developing serum-free medium. Specific applications, including using baculovirus and insect cell system to study apoptosis, are also discussed. This updated and expanded edition of Baculovirus Expression Protocols provides a perfect lab manual for biochemists, molecular biologists, biochemical engineers, and others for using the BEVS or insect cell lines to produce recombinant proteins
Analysis baculovirus
baculoviridae
insectenvirussen
insect viruses
genetica
genetics
genetische modificatie
genetic engineering
genetische vectoren
genetic vectors
genexpressie
gene expression
insecten
insects
cellen
cells
recombinant eiwitten
recombinant proteins
moleculaire biologie
molecular biology
biologische technieken
biological techniques
laboratoriummethoden
laboratory methods
bacterial insecticides
protocollen
protocols
Molecular Biology (General)
Genetic Engineering
Moleculaire biologie (algemeen)
Genetische modificatie
Notes Revised edition of: Baculovirus expression protocols. ©1995
Bibliography Includes bibliographical references and index
Notes Title from title screen (viewed November 20, 2007)
English
In Springer Protocols
Subject Baculoviruses -- Genetics.
Genetic engineering.
Virus-vector relationships.
Insect cell biotechnology.
Gene expression.
Genetics -- Technique.
Genetic vectors.
Baculoviridae -- genetics
Gene Expression
Genetic Techniques
Genetic Vectors
SCIENCE -- Life Sciences -- Biology.
SCIENCE -- Life Sciences -- Microbiology.
Insect cell biotechnology.
Baculoviridae -- genetics.
Gene expression.
Genetic Techniques.
Genetic vectors.
Baculoviruses -- Genetics.
Genetic engineering.
Virus-vector relationships.
Genetics -- Technique
Genetic vectors
Gene expression
Baculoviruses -- Genetics
Genetic engineering
Insect cell biotechnology
Virus-vector relationships
Genre/Form Laboratory manuals.
Manuels de laboratoire.
Form Electronic book
Author Murhammer, David W.
ISBN 1588295370
9781588295378
1597454575
9781597454575
1280971584
9781280971587
9786610971589
6610971587
9781617376290
1617376299
OTHER TI Baculovirus expression protocols