Description |
1 online resource (xiv, 470 pages) : illustrations (some color) |
Series |
Methods in molecular biology ; 388 |
|
Methods in molecular biology (Clifton, N.J.) ; v. 388
|
Contents |
Useful tips, widely used techniques, and quantifying cell metabolic behavior / D.W. Murhammer -- Introduction to baculovirus molecular biology / B.J. Kelly [and others] -- Baculovirus transfer vectors / R.D. Possee [and others] -- Recombinant baculovirus isolation / L.A. King [and others] -- Gene expression in mammalian cells using BacMam, a modified baculovirus system / J.A. Fornwald [and others] -- Available lepidopteran insect cell lines / D.E. Lynn -- Lepidopteran insect cell line isolation from insect tissue / D.E. Lynn -- Development of serum-free media for lepidopteran insect cell lines / S.N. Agathos -- Routine maintenance and storage of lepidopteran insect cell lines and baculoviruses / D.E. Lynn -- Small-scale protein production with the baculovirus expression vector system / S. Harwood -- Recombinant protein production in large-scale agitated bioreactors using the baculovirus expression vector system / C.B. Elias [and others] -- Production of baculovirus-expressed recombinant proteins in wave bioreactors / S.H. Kadwell [and others] -- Protein production with recombinant baculoviruses in lepidopteran larvae / Y. Liu [and others] -- Virus-like particle and viral vector production using the baculovirus expression vector system/insect cell system: Adeno-associated virus-based products / M.G. Aucoin [and others] -- Transforming lepidopteran insect cells for continuous recombinant protein expression / R.L. Harrison [and others] -- Transformation of drosophila cell lines: An alternative approach to exogenous protein expression / L. Cherbas [and others] -- Transforming lepidopteran insect cells for improved protein processing / R.L. Harrison [and others] -- Introduction to the use of baculoviruses as biological insecticides / B.C. Bonning [and others] -- Baculovirus insecticide production in insect larvae / N. van Beek [and others] -- Evaluation of the insecticidal efficacy of wild-type and recombinant baculoviruses / H. Li [and others] -- Monitoring and visualization of baculovirus infection using green fluorescent protein strategy / H.J. Cha [and others] -- Alternative bioreactor strategy for probing infection and production / Y.-C. Hu [and others] -- Methods for gene silencing with rnai / J.C. March [and others] -- Use of the baculovirus/insect cell system to study apoptosis / N. Chejanovsky -- Generation of envelope-modified baculoviruses for gene delivery into mammalian cells / C. Hofmann |
Summary |
Baculovirus Expression Protocols is a detailed guide for using the baculovirus expression vector system (BEVS) and/or insect cells to produce recombinant proteins. Procedures for the development and production of baculovirus insecticides at both laboratory and large scale are described in the kind of step-by-step format that the Methods in Molecular Biology series helped standardize. This book is a one-stop source for information on baculoviruses, and includes sections on using modified baculoviruses to express genes in mammalian cells, using Drosophila cell lines, isolating new cell lines, and developing serum-free medium. Specific applications, including using baculovirus and insect cell system to study apoptosis, are also discussed. This updated and expanded edition of Baculovirus Expression Protocols provides a perfect lab manual for biochemists, molecular biologists, biochemical engineers, and others for using the BEVS or insect cell lines to produce recombinant proteins |
Analysis |
baculovirus |
|
baculoviridae |
|
insectenvirussen |
|
insect viruses |
|
genetica |
|
genetics |
|
genetische modificatie |
|
genetic engineering |
|
genetische vectoren |
|
genetic vectors |
|
genexpressie |
|
gene expression |
|
insecten |
|
insects |
|
cellen |
|
cells |
|
recombinant eiwitten |
|
recombinant proteins |
|
moleculaire biologie |
|
molecular biology |
|
biologische technieken |
|
biological techniques |
|
laboratoriummethoden |
|
laboratory methods |
|
bacterial insecticides |
|
protocollen |
|
protocols |
|
Molecular Biology (General) |
|
Genetic Engineering |
|
Moleculaire biologie (algemeen) |
|
Genetische modificatie |
Notes |
Revised edition of: Baculovirus expression protocols. ©1995 |
Bibliography |
Includes bibliographical references and index |
Notes |
Title from title screen (viewed November 20, 2007) |
|
English |
In |
Springer Protocols |
Subject |
Baculoviruses -- Genetics.
|
|
Genetic engineering.
|
|
Virus-vector relationships.
|
|
Insect cell biotechnology.
|
|
Gene expression.
|
|
Genetics -- Technique.
|
|
Genetic vectors.
|
|
Baculoviridae -- genetics
|
|
Gene Expression
|
|
Genetic Techniques
|
|
Genetic Vectors
|
|
SCIENCE -- Life Sciences -- Biology.
|
|
SCIENCE -- Life Sciences -- Microbiology.
|
|
Insect cell biotechnology.
|
|
Baculoviridae -- genetics.
|
|
Gene expression.
|
|
Genetic Techniques.
|
|
Genetic vectors.
|
|
Baculoviruses -- Genetics.
|
|
Genetic engineering.
|
|
Virus-vector relationships.
|
|
Genetics -- Technique
|
|
Genetic vectors
|
|
Gene expression
|
|
Baculoviruses -- Genetics
|
|
Genetic engineering
|
|
Insect cell biotechnology
|
|
Virus-vector relationships
|
Genre/Form |
Laboratory manuals.
|
|
Manuels de laboratoire.
|
Form |
Electronic book
|
Author |
Murhammer, David W.
|
ISBN |
1588295370 |
|
9781588295378 |
|
1597454575 |
|
9781597454575 |
|
1280971584 |
|
9781280971587 |
|
9786610971589 |
|
6610971587 |
|
9781617376290 |
|
1617376299 |
|