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Title Adenoviral vectors for gene therapy / edited by David T. Curiel
Edition Second edition
Published Londonm UK : Academic Press is an imprint of Elsevier, 2016


Description 1 online resource : illustrations
Contents Front Cover; Adenoviral Vectors for Gene Therapy; Adenoviral Vectors for Gene Therapy; Copyright; Contents; List of Contributors; 1 -- Adenovirus Structure; 1. Historical Perspective on Adenovirus Structure; 2. Hexon Structure and Capsid Packing; 3. Penton Base Structure and Integrin-Binding RGD Loop; 4. Fiber Structure and Receptor Interactions; 5. Atomic Resolution Cryo-Electron Microscopy and X-ray Crystallographic Adenovirus Structures; 6. Hexons in the Atomic Resolution Adenovirus Structures; 7. Conformational Differences of the Penton Base in the Atomic Resolution Adenovirus Structures
8. Alternate Assignments for the Four-Helix Coiled Coil9. Protein IIIa Structure; 10. Protein IX Structure; 11. Core Protein V Structure; 12. Protein VI Structure; 13. Protein VIII Structure; 14. Adenovirus Protease; 15. Concluding Remarks; References; 2 -- Biology of Adenovirus Cell Entry: Receptors, Pathways, Mechanisms; 1. Introduction; 2. Entry Pathways: Impact of Capsid Proteins; 3. Attachment Factors and Signaling Receptors; 3.1 Accessing the CAR; 3.2 Using CD46 in High- and Low-Affinity Modes; 3.3 Desmoglein-2; 3.4 Sialic Acid; 3.5 Heparan Sulfate
3.6 Scavenger Receptors and Other Attachment Factors3.7 Internalization Co-receptors: Integrins; 3.8 Extracellular Factors Influencing Viral Tropism; 3.8.1 Coagulation Factors; 3.8.2 Immunoglobulins and Complement; 3.8.3 Lactoferrin; 3.8.4 Dipalmitoylphosphatidylcholine; 4. Endocytosis; 4.1 Clathrin-Mediated Endocytosis; 4.2 Macropinocytosis; 4.3 Phagocytosis; 4.4 Caveolar Endocytosis; 5. Endosomal Escape; 5.1 Protein VI for Membrane Lysis; 5.2 A New Concept: Hijacking Membrane Repair for Endosome Lysis; 6. Targeting the Nucleus; 6.1 Transport through the Cytoplasm
6.2 Deoxyribonucleic Acid Uncoating and Nuclear Import7. Conclusions and Perspectives; Acknowledgments; References; 3 -- Adenovirus Replication; 1. Introduction; 2. Classification; 3. Adenovirus Genome Organization; 4. Virus Infection; 5. Early Gene Expression; 5.1 Early Region 1A; 5.2 Early Region 1B; 5.3 Early Region 2; 5.4 Early Region 3; 5.5 Early Region 4; 6. Viral DNA Replication; 7. Virus-Associated RNA Genes; 8. Late Gene Expression; 9. Viral DNA Packaging; 10. Conclusion; References; 4 -- Adenoviral Vector Construction I: Mammalian Systems; 1. Introduction; 1.1 Adenovirus Biology
1.2 Adenovirus Vectors2. Cell Lines for Propagating Adenovirus Vectors; 2.1 Propagation of Adenovirus Vectors Encoding Toxic Transgenes for Cancer Gene Therapy; 3. Construction of First-Generation Adenoviral Vectors; 3.1 Early Methods; 3.2 The Two-Plasmid Rescue System; 3.2.1 Development of the Two-Plasmid Rescue System; 3.2.2 Fine-Tuning of the Two-Plasmid Rescue System; 4. Steps Involved in Adenovirus Vector Construction; 4.1 Preparation of Adenovirus Genomic and Shuttle Plasmid DNA for Cotransfection; 4.2 Cotransfection of HEK-293 Cells with Genomic and Shuttle Plasmid
Summary "[This book] provides detailed, comprehensive coverage of the gene delivery vehicles that are based on the adenovirus that is emerging as an important tool in gene therapy. These exciting new therapeutic agents have great potential for the treatment of disease, making gene therapy a fast-growing field for research. This book presents topics ranging from the basic biology of adenoviruses, through the construction and purification of adenoviral vectors, cutting-edge vectorology, and the use of adenoviral vectors in preclinical animal models, with final consideration of the regulatory issues surrounding human clinical gene therapy trials. This broad scope of information provides a solid overview of the field, allowing the reader to gain a complete understanding of the development and use of adenoviral vectors."--Website publisher
Bibliography Includes bibliographical references
Notes Online resource; title from PDF title page (ScienceDirect, viewed March 22, 2016)
Subject Gene therapy.
Genetic Therapy -- methods
Genetic Therapy
HEALTH & FITNESS -- Diseases -- Genetic.
MEDICAL -- Genetics.
Gene therapy
Form Electronic book
Author Curiel, David, editor.
ISBN 9780128005101