Description |
1 online resource (xii, 228 pages) : illustrations |
Series |
Methods in molecular biology ; v. 229 |
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Methods in molecular biology (Clifton, N.J.) ; v. 229
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Contents |
From lentiviruses to lentivirus vectors / M. Federico -- The choice of a suitable lentivirus vector: Transcriptional targeting / F. Lotti and F. Mavilio -- Choice and use of appropriate packaging cell types / A.L. Pacchia, S. Mukherjee and J.P. Dougherty -- Small- to large-scale production of lentivirus vectors / M.P. Marino, M.J. Luce and J. Reiser -- Detection and titration of lentivirus vector preparations / J. Gatlin, M. Islas-Ohlmayer and J.V. Garcia -- Detection and selection of lentiviral vector-transduced cells / Y. Cui and L.J. Chang -- Detection of replication-competent lentiviral particles / H. Segall and R.E. Sutton -- Lymphocytes / D. Chinnasamy and F. Candotti -- Monocyte/macrophages and dendritic cells / H. Segall and R.E. Sutton -- Hematopoietic stem and progenitor cells / R.E. Donahue and I.S. Chen -- Mesenchymal stem cells / X.Y. Zhang, V.F. La Russa and J. Reiser -- Hippocampal neurons / J.L. Nadeau -- Dorsal root ganglia sensory neurons / J. Fleming, S.L. Ginn and I.E. Alexander -- Cardiomyocytes / D. Bonci, M.V. Latronico and G. Condorelli -- Airway epithelia / L.G. Johnson, S.H. Randell and J.C. Olsen -- Corneal cells / B. Appukuttan, T. McFarland and J.T. Stout -- Retinal tissue / T. McFarland, B. Appukuttan and J.T. Stout -- Hiv-2 vectors / S.K. Arya, J.E. Cho and S.H. Chang -- SIV vectors / A. Ruggieri, D. Negre and F.L. Cosset -- FIV vectors / N. Loewen [and others] -- Hybrid lentivirus vectors / V. Planelles -- Cells of respiratory epithelium / P.L. Sinn and P.B. McCray, Jr. -- Cells of the nervous system / C. Rosenblad and C. Lundberg |
Summary |
Since the publication of the first edition, lentivirus vector-based technologies, through in vitro and in vivo gene transfer in eukaryotic animal cells, continue to offer the most promising opportunities for curing genetic disorders, as well as cancer and infectious diseases. Lentivirus Gene Engineering Protocols, Second Edition reflects the spectacular progress made in the field with a set of cutting-edge methods contributed by highly respected scientists. Beginning with a thorough overview of the most recent lentivirus developments, the book continues with detailed protocols including sections on the advances in lentiviral vector technology, new lentiviral vector applications, involving transgenic human embryonic stem cells and fetal gene therapy among other topics, as well as the invaluable breakthroughs in LV-mediated expression of microRNAs. Written in the highly successful Methods in Molecular Biology™ series format, chapters include introductions to their respective subjects, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and notes sections, highlighting tips on troubleshooting and avoiding known pitfalls. Authoritative and timely, Lentivirus Gene Engineering Protocols, Second Edition covers the most relevant issues and techniques of LV-based gene engineering, thus representing a complete theoretical and practical guide for scientists still unfamiliar with LV technologies and those who simply wish to know more about this vital area of study |
Analysis |
lentivirus |
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celbiologie |
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cellular biology |
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genetische modificatie |
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genetic engineering |
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laboratoriummethoden |
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laboratory methods |
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protocollen |
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protocols |
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Genetic Engineering |
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Genetische modificatie |
Bibliography |
Includes bibliographical references and index |
Notes |
English |
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Print version record |
Subject |
Genetic engineering.
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Lentiviruses.
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Genetic vectors.
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Lentivirus -- genetics
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Gene Targeting -- methods
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Gene Transfer Techniques
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Genetic Engineering -- methods
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Genetic Vectors -- biosynthesis
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Lentivirus
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Genetic Vectors
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Genetic engineering
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Genetic vectors
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Lentiviruses
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Genre/Form |
Laboratory manuals
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Laboratory manuals.
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Manuels de laboratoire.
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Form |
Electronic book
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Author |
Federico, Maurizio
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LC no. |
2009942262 |
ISBN |
9781607615330 |
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1607615339 |
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9781607615323 |
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1607615320 |
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