Title Gene therapy protocols / edited by Jeffrey R. Morgan

Edition 2nd ed Published Totowa, N.J. : Humana Press, 2002

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Description 1 online resource (xv, 520 pages) : illustrations Series Methods in molecular medicine ; 69 Methods in molecular medicine ; 69. Contents Poly-l-lysine-based gene delivery systems. Synthesis, purification, and application / C.P. Lollo [and others]-Immunizing potential of cytokine-transduced tumor cells / M.P. Colombo and M. Rodolfo-Targeted gene transfer to liver using protein-DNA complexes / C.H. Wu, C.M. Walton and G.Y. Wu-Receptor-directed molecular conjugates for gene transfer / A.G. Ziady and P.B. Davis-Particle-mediated gene transfer into dendritic cells : A novel strategy for the induction of immune responses against tumor antigens / T. Tuting and A. Albers-Cancer gene therapy with heat shock protein-65 gene / K.V. Lukacs and A. Nakakes-Gene transfer into muscle by electroporation in vivo / J. Miyazaki and H. Aihara-Recombinant vaccinia virus mva for generation and analysis of t cell responses against tumor associated antigens / I. Drexler [and others]-Viral liposomes. Preparation and use / Y. Kaneda-Lpd nanoparticles--novel nonviral vector for efficient gene delivery / Y. Tan [and others]-Selection of cytochrome p450 genes for use in prodrug activation-based cancer gene therapy / J.E. Hecht and D.J. Waxman-Solvoplex synthetic vector for intrapulmonary gene delivery. Preparation and use / K. Schughart and U.B. Rasmussen-Construction of p450-expressing tumor cell lines using retroviruses / J.E. Hecht, Y. Jounaidi and D.J. Waxman-7 in vitro methods for evaluation of p450-based anticancer gene therapy / D.H. JE and D.J. Waxman-Gene correction frequency by chimeric rna-DNA oligonucleotide using nuclear extracts / O.A. Igoucheva and K. Yoon-8 tumor models for evaluation of p450 gene therapy in vivo / J.E. Hecht, P. Schreiber Schwartz and D.J. Waxman-Regulated expression of plasmid-based gene therapies / R.V. Abruzzese [and others]-9 intracellular single-chain antibodies for gene therapy / G. Bilbao [and others]-Photochemical transfection. Light-induced, site-directed gene delivery / L. Prasmickaite, A. Hogset and K. Berg-Direct gene transfer and vaccination via skin transfection using a gene gun / C.F. Kuo, J.H. Wang and N.S. Yang-Preparation of pseudotyped retroviral vector / H. Yu-Combined adenoviral transfer of tumor suppressor and cell-cycle genes for tumor-cell apoptosis / K. Brand, V. Sandig and M. Strauss-Quantitative measurement of the concentration of active recombinant retrovirus / S.T. Andreadis and J.R. Morgan-Inhibition of cell growth by antisense oligonucleotides targeting the growth-related protein kinase c-raf / D. Fabbro [and others]-Minigene-containing retroviral vectors using an alphavirus/retrovirus hybrid vector system. Production and use / J. Wahlfors and R.A. Morgan-Retrovirus-mediated gene transfer to human hematopoietic stem cells / W.P. Swaney [and others]-Igf-1 antisense strategies for cancer treatment / Y.X. Pan and D.D. Anthony-Genetically modified skin substitutes. Preparation and use / K.E. Hamoen [and others]-Anti-mdr1 ribozyme gene therapy / T. Ohnuma, H. Kobayashi and F.S. Wang-Bioartificial muscles in gene therapy / C. Powell [and others]-Cytokine gene-modified cell-based cancer vaccines / R.T. Reilly [and others]-Anti-c-erb1b2 ribozyme for gene therapy of breast cancer / T. Suzuki, M. Bessho and K.J. Scanlon-Hiv-based vectors. Preparation and use / A. Follenzi and L. Naldini-Anti-k-ras1 ribozyme adenoviral vector for gene therapy of non-small cell lung cancer / Y.A. Zhang, J. Nemunaitis and A.W. Tong-Green fluorescent protein retroviral vector : Generation of high-titer producer cells and virus supernatant / W. Uckert, L. Pedersen and W. Gunzburg-Packaging cell system for lentivirus vectors. Preparation and use / N. Srinivasakumar-Hsv-1 vectors for gene therapy of experimental cns tumors / U. Herrlinger [and others]-Lentiviral vectors. Preparation and use / L.J. Chang and A.K. Zaiss-Intratumoral injection of naked DNA / J. Yang-Simian foamy virus vectors. Preparation and use / J. Park and A. Mergia-Cationic liposome gene transfer / K. Kay Son-In vivo particle-mediated gene transfer for cancer therapy / A.L. Rakhmilevich and N.S. Yang-Recombinant feline immunodeficiency virus vectors. Preparation and use / M.A. Curran and G.P. Nolan-Gene targeting to hepatomas (afp) / S. Tsuruta, A. Ido and S. Nagataki-Lentivirus vector based on simian immunodeficiency virus. Development and use / K. Uberla-Adenovirus-mediated targeted gene therapy for breast cancer and for purging hematopoietic stem-cell sources / L. Chen-Cytoplasmic rna vector derived from nontransmissible sendai virus. Production and use / A. Iida and M. Hasegawa-Preparation of helper-dependent adenoviral vectors / P. Ng, R.J. Parks and F.L. Graham-Chemotherapy-inducible vector for gene therapy of cancer / W. Walther [and others]-Construction of first-generation adenoviral vectors / P. Ng and F.L. Graham-Oncolytic adenoviral vectors / R. Alemany and W.W. Zhang-Genetically modified clostridium for gene therapy of tumors / M.E. Fox [and others]-Preparation of ovine adenovirus vectors / P. Loser [and others]-Tumor-targeted salmonella: Strain development and expression of the hsv-tk effector gene / D. Bermudes, B. Low and J.M. Pawelek-Highly purified recombinant adeno-associated virus vectors. Preparation and quantitation / B. Schnepp and K.R. Clark-Ex vivo cytokine gene transfer in melanomas by using particle bombardment / D. Schadendorf-High-titer stocks of adeno-associated virus from replicating amplicons and herpes vectors / A.R. Mistry [and others]-Intratumoral gene transfer of the hla-b7 gene into colon carcinoma metastases / E. Galanis and J. Rubin-Herpes simplex virus/adeno-associated virus hybrid vectors for gene transfer to neurons. Preparation and use / L.C. Costantini [and others]-Hybrid cell vaccination in patients with metastatic melanoma / U. Trefzer [and others]-Retroviral transfer of the herpes simplex virus-thymidine kinase (hsv-tk) gene for the treatment of cancer / R. Ramesh [and others]-Development of replication-defective herpes simplex virus vectors / W.F. Goins [and others]-Gene therapy for treatment of brain tumors (hsv-tkin vivo gene transfer) : A case study / F. Weber, F. Floeth and H. Bojar-Gene therapy of glioblastoma multiforme with a bicistronic retroviral vector expressing human il-2 and hsv-tk / G. Palu [and others]-Intratumoral gene transfer of the cytosine deaminase gene for the treatment of breast cancer / H.S. Pandha and N.R. Lemoine-Adenovirus-mediated wild-type p53 gene transfer into head and neck cancers / G.L. Clayman, D.K. Frank and P.A. Bruso-Direct DNA injection (p53) into hcc tumors / R.R. Mitry and N.A. Habib-A phase ii trial of intratumoral injection with a selectively replicating adenovirus (onyx-015) in patients with recurrent, refractory squamous cell carcinoma of the head and neck / D.H. Kirn-C-myb antisense oligonucleotide therapeutics for hematologic malignancies / S. Luger-Ovarian cancer gene therapy with brca1-an overview / P.S. Obermiller and J.T. Holt-Methods for chemoprotection and chemosensitization : Mdr-1 for chemoprotection using retroviruses to modify hematopoietic cells and cytosine deaminase for chemosensitization using adenoviral vectors to modify epithelian neoplastic cells / S. Shrimdkandada [and others] Summary Significant advances in gene transfer technology and the completion of sequencing the human genome offer much renewed hope that gene therapy will provide novel approaches to the treatment of inherited and acquired diseases. In this fully updated and revised 2nd edition of the warmly received Gene Therapy Protocols, leading experts from academic and industrial laboratories around the world detail their most effective viral and nonviral methods of gene transfer, as well as discuss their applications in different organ systems. The methods range from those in which new molecular conjugates show great promise for targeting targeting gene transfer and regulating transgene expression, to those used in such exciting applications as the delivery of therapeutic proteins, vaccination, and tissue engineering. In addition, there are completely revised methods based on retroviruses and adenoviruses, as well as new and promising methods based on lentiviruses and adeno-associated viruses. The quantitative aspects of gene therapy are strongly emphasized, and the book reflects the many significant recent improvements in gene transfer efficiency, gene regulation, and vector production. Up-to-date and highly practical, Gene Therapy Protocols, 2nd Edition, offers a rich compilation of the revolutionary advances that have recently occurred in gene transfer technology, with each article providing proven step-by-step laboratory procedures that enable successful therapeutic application. -- Provided by publisher Analysis gentherapie gene therapy geneeskunde medicine genen genes biologische technieken biological techniques protocollen protocols humane genetica human genetics Medicine (General) Molecular Genetics Geneeskunde (algemeen) Moleculaire genetica Bibliography Includes bibliographical references and index Notes English Print version record Subject Gene therapy -- Methodology Genetic vectors. Genetic Therapy -- methods Gene Transfer Techniques Genetic Vectors HEALTH & FITNESS -- Diseases -- Genetic. MEDICAL -- Genetics. Genetic vectors Gentherapie. Wetenschappelijke technieken. Genre/Form Laboratory manuals. Manuels de laboratoire. Form Electronic book Author Morgan, Jeffrey Robert ISBN 9781592591411 1592591418 128082106X 9781280821066 9786610821068 6610821062 9781489938763 1489938761

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