| Description |
1 online resource (xi, 272 pages) : illustrations (some color) |
| Series |
Methods in molecular biology, 1064-3745 ; 1605 |
|
Methods in molecular biology (Clifton, N.J.) ; 1605. 1064-3745
|
| Contents |
Clearance of maternal RNAs : not a Mummy's embryo anymore / Antonio Marco -- Link of zygotic genome activation and cell cycle control / Boyang Liu and Jörg Grosshans -- Role of microRNAs in zygotic genome activation : modulation of mRNA during embryogenesis / Alessandro Rosa and Ali H. Brivanlou -- Gene expression analysis in mammalian oocytes and embryos by quantitative real-time RT-PCR / Kyeoung-Hwa Kim, Su-Yeon Lee, and Kyung-Ah Lee -- Detection of miRNA in mammalian oocytes and embryos / Malavika K. Adur, Benjamin J. Hale, and Jason W. Ross -- Detection of bidirectional promoter-derived lncRNAs from small-scale samples using pre-amplification-free directional RNA-seq Method / Nobuhiko Hamazaki, Kinichi Nakashima, Katsuhiko Hayashi, and Takuya Imamura -- Detection and characterization of small noncoding RNAs in mouse gametes and embryos prior to zygotic genome activation / Jesús García-López, Eduardo Larriba, and Jesús del Mazo -- Purification of zygotically transcribed RNA through metabolic labeling of early zebrafish embryos / Patricia Heyn and Karla M. Neugebauer -- RNA FISH to study zygotic genome activation in early mouse embryos / Noémie Ranisavljevic, Ikuhiro Okamoto, Edith Heard, and Katia Ancelin -- Detection of RNA polymerase II in mouse embryos during zygotic genome activation using immunocytochemistry / Irina O. Bogolyubova and Dmitry S. Bogolyubov -- Immunological staining of global changes in DNA methylation in the early mammalian embryo / Yan Li and Christopher O'Neill -- Single cell restriction enzyme-based analysis of methylation at genomic imprinted regions in preimplantation mouse embryos / Ka Yi Ling, Lih Feng Cheow, Stephen R. Quake, William F. Burkholder, and Daniel M. Messerschmidt -- Use of chemicals to inhibit DNA replication, transcription, and protein synthesis to study zygotic genome activation / Kyungjun Uh and Kiho Lee -- Targeted gene knockdown in early embryos using siRNA / Lu Zhang and Zoltan Machaty -- Generating mouse models using zygote electroporation of nucleases (ZEN) technology with high efficiency and throughput / Wenbo Wang, Yingfan Zhang, and Haoyi Wang -- CRISPR/Cas9-mediated gene targeting during embryogenesis in swine / Junghyun Ryu and Kiho Lee -- Potential involvement of SCF-complex in zygotic genome activation during early bovine embryo development / Veronika Benesova, Veronika Kinterova, Jiri Kanka, and Tereza Toralova -- Use of histone K-M mutants for the analysis of transcriptional regulation in mouse zygotes / Keisuke Aoshima, Takashi Kimura, and Yuki Okada |
| Summary |
This volume provides an overview of zygotic genome activation (ZGA) and the use of recent tools to elucidate the events that occur during ZGA. The chapters in this book cover topics such as: clearance of maternal RNAs, detection of miRNA in mammalian oocytes and embryos, RNA FISH to study ZGA in early mouse embryos, and CRISPR/Cas9-mediated gene targeting during embryogenesis in the mouse and pig. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Thorough and cutting-edge, Zygotic Genome Activation: Methods and Protocols is a valuable resource for scientists and researchers who are interested in learning more about practical approaches to monitor the changes seen during ZGA |
| Notes |
Includes index |
| Bibliography |
Includes bibliographical references and index |
| Notes |
Online resource; title from PDF title page (SpringerLink, viewed May 2, 2017) |
| Subject |
Developmental genetics -- Laboratory manuals
|
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Zygotes -- Laboratory manuals
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Gene expression -- Laboratory manuals
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Genomes.
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Genetic transcription.
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Transcriptional Activation
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Genome
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Zygote -- metabolism
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Genes, Developmental
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Transcription, Genetic
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Gene Expression Regulation, Developmental
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Medical genetics.
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Medical -- Genetics.
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Genomes
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Genetic transcription
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Developmental genetics
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Gene expression
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Zygotes
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| Genre/Form |
Laboratory manuals
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Laboratory manuals.
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Manuels de laboratoire.
|
| Form |
Electronic book
|
| Author |
Lee, Kiho, editor
|
| LC no. |
2017937534 |
| ISBN |
9781493969883 |
|
1493969889 |
|
