Title Molecular analysis of cancer / edited by Jacqueline Boultwood and Carrie Fidler

Published Totowa, N.J. : Humana Press, ©2002

Click on the following:
Springer eBooks

Copies

Description 1 online resource (xi, 302 pages) : illustrations (some color) Series Methods in molecular medicine ; 68 Methods in molecular medicine ; 68. Contents Molecular analysis of cancer. An overview -- Detection of chromosome abnormalities in leukemia using fluorescence in situ hybridization -- Spectral karyotyping in cancer cytogenetics -- Comparative genomic hybridization analysis -- Detection of chromosomal deletions by microsatellite analysis -- Detection and quantification of leukemia-specific rearrangements -- Detection of t(2;5)(p23;q35) translocation by long-range PCR of genomic DNA -- Use of DNA fingerprinting to detect genetic rearrangements in human cancer -- Mutation analysis of large genomic regions in tumor DNA using single-strand conformation polymorphism. Lessons from the ATM gene -- Mutational analysis of oncogenes and tumor suppressor genes in human cancer using denaturing gradient gel electrophoresis -- Detection of mutations in human cancer using nonisotopic RNase cleavage assay -- Mutational analysis of the neurofibromatosis type 1 gene in childhood myelodysplastic syndromes using a protein truncation assay -- Mutation analysis of cancer using automated sequencing -- Detection of differentially expressed genes in cancer using differential display -- Genomewide gene expression analysis using cDNA microarrays -- Gene expression profiling in cancer using cDNA microarrays -- Wilms tumor gene WT1 as a tumor marker for leukemic blast cells and its role in leukemogenesis -- Detection of aberrant methylation of the p15INK4B gene promoter -- Clonality studies in cancer based on X chromosome inactivation phenomenon -- Telomere length changes in human cancer -- Measurement of telomerase activity in human hematopoietic cells and neoplastic disorders Summary Molecular biotechnology has in recent years produced significant advances in finding tumor markers useful in diagnosis and in the assessment of disease progression and prognosis. In Molecular Analysis of Cancer, leading researchers in the field describe in step-by-step detail their best state-of-the-art molecular techniques for elucidating the biochemical processes associated with human cancer. Among the techniques presented for identifying chromosomal rearrangements are fluorescent in situ hybridization (FISH), spectral karyotyping (SKY), comparative genomic hybridization (CGH), microsatellite analysis, PCR and RT-PCR, and DNA fingerprinting. Additional methods include SSCP, DGGE, the nonisotopic RNase cleavage assay, and the protein truncation assay and DNA sequencing for identifying mutations. Techniques for the analysis of gene expression include microarray technology and differential display. All these techniques are of great value for identifying genes that are central to cancer development and progression. Comprehensive and state-of-the-art, Molecular Analysis of Cancer not only offers today's researcher a deeper understanding of cancer's molecular pathogenesis, but also provides a detailed examination of each of the major methodologies needed to detect and analyze its associated genetic abnormalities Bibliography Includes bibliographical references and index Notes Master and use copy. Digital master created according to Benchmark for Faithful Digital Reproductions of Monographs and Serials, Version 1. Digital Library Federation, December 2002. http://purl.oclc.org/DLF/benchrepro0212 MiAaHDL English Print version record digitized 2010 HathiTrust Digital Library committed to preserve pda MiAaHDL In Springer Protocols Subject Cancer -- Genetic aspects -- Research -- Methodology Cancer -- Molecular aspects -- Research -- Methodology Genetics -- Technique. Neoplasms -- genetics Cell Transformation, Neoplastic -- genetics Gene Expression Regulation, Neoplastic Genetic Techniques MEDICAL -- Oncology. HEALTH & FITNESS -- Diseases -- Cancer. Medicine Pathology Krebs Medizin Molekulare Medizin Kanker. Moleculaire biologie. Wetenschappelijke technieken. Genre/Form Laboratory manuals. Manuels de laboratoire. Form Electronic book Author Boultwood, Jacqueline. Fidler, Carrie. ISBN 9781592591350 1592591353 0585438919 9780585438917 9786610821020 661082102X 1280821027 9781280821028

  Permalink