| Description |
1 online resource (xi, 248 pages) : illustrations |
| Series |
Methods in molecular biology, 1064-3745 ; 354 |
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Methods in molecular biology (Clifton, N.J.) ; v. 354.
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| Contents |
The use of protoplasts to study innate immune responses / Ping He, Libo Shan, and Jen Sheen -- Marker-exchange mutagenesis and complementation strategies for the gram-negative bacteria Xanthomonas oryzae pv. oryzae / Sang-Won Lee and Pamela C. Ronald -- Whole-genome analysis to identify type III-secreted effectors / Boris A. Vinatzer and Jean T. Greenberg -- In planta expression of oomycete and fungal genes / Thirumala-Devi Kanneganti, Edgar Huitema, and Sophien Kamoun -- Use of Nipponbare BAC clones for physical mapping of an R gene locus in rice / Jong-Seong Jeon and Pamela C. Ronald -- Agrobacterium-mediated transformation to create an insertion library in Magnaporthe grisea / Sara L. Tucker and Marc J. Orbach -- Identification of components in disease-resistance signaling in arabidopsis by map-based cloning / Yuelin Zhang, Jane Glazebrook, and Xin Li -- Yeast two-hybrid approaches to dissecting the plant defense response / Mawsheng Chern, Todd Richter, and Pamela C. Ronald -- Use of rolling-circle amplification for large-scale yeast two-hybrid analyses / Xiaodong Ding, Yan Zhang, and Wen-Yuan Song -- Preparative denaturing isoelectric focusing for enhancing sensitivity of proteomic studies / Antonio Serna-Sanz, Greg Rairdan, and Scott C. Peck |
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Use of massively parallel signature sequencing to study genes expressed during the plant defense response / Blake C. Meyers, Christian D. Haudenschild, and Kalyan Vemaraju -- Use of microarray analysis to dissect the plant defense response / Jane Glazebrook -- Use of robust-long serial analysis of gene expression to identify novel fungal and plant genes Involved in host-pathogen interactions / Malali Gowda [and others] -- Analysis of gene function in rice through virus-induced gene silencing / Xin Shun Ding, C. Srinivasa Rao, and Richard S. Nelson -- Use of RNA interference to dissect defense-signaling pathways in rice / Chuansheng Mei, Xiangjun Zhou, and Yinong Yang -- Virus-induced gene silencing in plant roots / Isgouhi Kaloshian -- Methods for engineering resistance to plant viruses / Mysore R. Sudarshana, Gourgopal Roy, and Bryce W. Falk -- Development of genetically engineered resistant papaya for papaya ringspot virus in a timely manner : a comprehensive and successful approach / Savarni Tripathi, Jon Suzuki, and Dennis Gonsalves |
| Summary |
Plant-Pathogen Interactions: Methods and Protocols provides key methods, approaches, and strategies to dissect the plant defense response. Addressing methods to identify and characterize plant resistance genes as well as pathogen-associated molecules that trigger the plant defense response, this volume creates a better understanding of the interactions between pathogens and their hosts, which will help to develop better methods for disease control in plants and animals. Plant-Pathogen Interactions: Methods and Protocols reviews methods for engineering resistance to plant viruses, the utility of viral induced gene silencing and RNAi silencing, as well as advances in genomics and proteomics that have lead to new methods to identify genes and proteins. The protocols presented follow the successful Methods in Molecular Biology series format, each one offering step-by-step laboratory instructions, an introduction outlining the principle behind the technique, lists of equipment and reagents, and tips on troubleshooting and the avoidance of known pitfalls |
| Bibliography |
Includes bibliographical references and index |
| Notes |
Print version record |
| In |
OhioLINK electronic book center |
|
SpringerLink |
| Subject |
Plant-pathogen relationships -- Molecular aspects -- Research -- Methodology
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Plant diseases -- Molecular aspects -- Research -- Methodology
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Plant Diseases -- microbiology
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Gene Expression Regulation, Plant -- immunology
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Gene Silencing
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Immunity, Innate -- genetics
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Plant Diseases -- genetics
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TECHNOLOGY & ENGINEERING -- Agriculture -- General.
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Plant Diseases -- microbiology.
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Gene Expression Regulation, Plant -- immunology.
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Gene silencing.
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Immunity, Natural -- genetics.
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Plant Diseases -- genetics.
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Plant-pathogen relationships -- Molecular aspects -- Research -- Methodology.
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Plant diseases -- Molecular aspects -- Research -- Methodology.
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| Genre/Form |
Laboratory manuals.
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Manuels de laboratoire.
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| Form |
Electronic book
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| Author |
Ronald, Pamela C
|
| LC no. |
2006007133 |
| ISBN |
158829448X |
|
9781588294487 |
|
1592599664 |
|
9781592599660 |
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