Title Principles and technical aspects of PCR amplification / Elizabeth van Pelt-Verkuil, Alex van Belkum, John P. Hays

Published [Dordrecht] : Springer, 2008

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Description 1 online resource (325 pages) : illustrations Contents The Polymerase Chain Reaction -- A Brief Comparison Between In Vivo DNA Replication and In Vitro PCR Amplification -- The PCR in Practice -- The Different Types and Varieties of Nucleic Acid Target Molecules -- PCR Primers -- Deoxynucleotide Triphosphates and Buffer Components -- Taq and Other Thermostable DNA Polymerases -- Important Considerations for Typical, Quantitative and Real-Time PCR Protocols -- Analysis of PCR Amplification Products -- Ensuring PCR Quality -- Laboratory Organisation, PCR Optimization and Controls -- Ensuring PCR Quality -- Quality Criteria and Quality Assurance -- Variants and Adaptations of the Standard PCR Protocol -- In Situ PCR Amplification (ISA) -- Major Considerations, Sample Processing and Applications Summary "This manual aims to provide the reader with a guide to the standard PCR technique and its many available modifications, with particular emphasis on the role of PCR techniques in the diagnostic laboratory (the central theme of this manual). Further, many important technical issues have been addressed, including types of PCR template material, PCR optimization, the analysis of PCR products, quality control, quantitative PCR and in situ PCR. The reader of this manual will be thoroughly informed about the fundamental principles of PCR and the true potential of PCR within clinical laboratory practice."--Jacket Bibliography Includes bibliographical references and index Notes Print version record Subject Polymerase chain reaction -- Laboratory manuals Polymerase chain reaction -- Diagnostic use. Gene amplification. Polymerase chain reaction. Gene Amplification Polymerase Chain Reaction SCIENCE -- Life Sciences -- Genetics & Genomics. Biomédecine. Sciences de la vie. Gene amplification Polymerase chain reaction Polymerase chain reaction -- Diagnostic use Genre/Form Laboratory manuals Laboratory manuals. Manuels de laboratoire. Form Electronic book Author Belkum, Alex van. Hays, John P. ISBN 9781402062414 1402062419 9781402062407 1402062400 1281242373 9781281242372

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