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Author Pelt-Verkuil, Elizabeth van, 1951-

Title Principles and technical aspects of PCR amplification / Elizabeth van Pelt-Verkuil, Alex van Belkum, John P. Hays
Published [Dordrecht] : Springer, 2008


Description 1 online resource (325 pages) : illustrations
Contents The Polymerase Chain Reaction -- A Brief Comparison Between In Vivo DNA Replication and In Vitro PCR Amplification -- The PCR in Practice -- The Different Types and Varieties of Nucleic Acid Target Molecules -- PCR Primers -- Deoxynucleotide Triphosphates and Buffer Components -- Taq and Other Thermostable DNA Polymerases -- Important Considerations for Typical, Quantitative and Real-Time PCR Protocols -- Analysis of PCR Amplification Products -- Ensuring PCR Quality -- Laboratory Organisation, PCR Optimization and Controls -- Ensuring PCR Quality -- Quality Criteria and Quality Assurance -- Variants and Adaptations of the Standard PCR Protocol -- In Situ PCR Amplification (ISA) -- Major Considerations, Sample Processing and Applications
Summary "This manual aims to provide the reader with a guide to the standard PCR technique and its many available modifications, with particular emphasis on the role of PCR techniques in the diagnostic laboratory (the central theme of this manual). Further, many important technical issues have been addressed, including types of PCR template material, PCR optimization, the analysis of PCR products, quality control, quantitative PCR and in situ PCR. The reader of this manual will be thoroughly informed about the fundamental principles of PCR and the true potential of PCR within clinical laboratory practice."--Jacket
Bibliography Includes bibliographical references and index
Notes Print version record
Subject Polymerase chain reaction -- Laboratory manuals
Polymerase chain reaction -- Diagnostic use.
Gene amplification.
Polymerase chain reaction.
Gene Amplification
Polymerase Chain Reaction
SCIENCE -- Life Sciences -- Genetics & Genomics.
Sciences de la vie.
Gene amplification.
Polymerase chain reaction.
Polymerase chain reaction -- Diagnostic use.
Genre/Form Laboratory manuals.
Laboratory manuals.
Manuels de laboratoire.
Form Electronic book
Author Belkum, Alex van.
Hays, John P.
ISBN 9781402062414