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Title Laboratory protocols in fungal biology : current methods in fungal biology / Vijai Kumar Gupta, Maria G. Tuohy, editors ; Manimaran Ayyachamy, Kevin M. Turner, Anthonia O'Donovan, associate editors
Published New York : Springer, [2013]
Online access available from:
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Description 1 online resource (289 pages) : illustrations
Series Fungal biology
Fungal biology (Springer (Firm))
Contents Safety Norms and Regulations in Handling Fungal Specimens / Finola E. Cliffe -- Methods of Cryopreservation in Fungi / Ladislav Homolka -- Long-Term Preservation of Fungal Cultures in All-Russian Collection of Microorganisms (VKM): Protocols and Results / Svetlana M. Ozerskaya, Natalya E. Ivanushkina -- Fungal Specimen Collection and Processing / Anthonia O'Donovan, Vijai Kumar Gupta -- Chemical and Molecular Methods for Detection of Toxigenic Fungi and Their Mycotoxins from Major Food Crops / S. Chandra Nayaka, M. Venkata Ramana -- Identification Key for the Major Growth Forms of Lichenized Fungi / Jeyabalan Sangeetha, Devarajan Thangadurai -- Microscopic Methods for Analytical Studies of Fungi / De-Wei Li -- Scanning Electron Microscopy for Fungal Sample Examination / Eduardo Alves, Gilvaine Ciavareli Lucas -- High-Resolution rImaging and Force Spectroscopy of Fungal Hyphal Cells by Atomic Force Microscopy / Biplab C. Paul, Hui Ma, Laelie A. Snook
Use of Fourier-Transform Infrared (FTIR) Microscopy Method for Detection of Phyto-Fungal Pathogens / Vitaly Erukhimovitch, Mahmoud Huleihel -- Diagnosis of Parasitic Fungi in the Plankton: Technique for Identifying and Counting Infective Chytrids Using Epifluorescence Microscopy / Télesphore Sime-Ngando, Serena Rasconi -- Fungal Cell Wall Analysis / Pilar Pérez, Juan C. Ribas -- Histopathological Technique for Detection of Fungal Infections in Plants / Vijai Kumar Gupta, Brejesh Kumar Pandey -- Development of Media for Growth and Enumeration of Fungi from Water / Segula Masaphy -- Sabouraud Agar for Fungal Growth / Janelle M. Hare -- A Method for the Formation of Candida Biofilms in 96 Well Microtiter Plates and Its Application to Antifungal Susceptibility Testing / Christopher G. Pierce, Priya Uppuluri -- Screening for Compounds Exerting Antifungal Activities / Jean-Paul Ouedraogo, Ellen L. Lagendijk -- Fluorescence In Situ Hybridization of Uncultured Zoosporic Fungi / Télesphore Sime-Ngando, Marlène Jobard
Staining Techniques and Biochemical Methods for the Identification of Fungi / Jeyabalan Sangeetha, Devarajan Thangadurai -- Protocol for the In Vivo Quantification of Superoxide Radical in Fungi / Konstantinos Grintzalis, Ioannis Papapostolou -- Isolation of Intact RNA from Sorted S. cerevisiae Cells for Differential Gene Expression Analysis / Jeannette Vogt, Frank Stahl, Thomas Scheper -- Quantitative PCR Analysis of Double-Stranded RNA-Mediated Gene Silencing in Fungi / José J. de Vega-Bartol, Vega Tello, Jonathan Niño -- Semi-Nested PCR Approach to Amplify Large 18S rRNA Gene Fragments for PCR-DGGE Analysis of Soil Fungal Communities / Miruna Oros-Sichler, Kornelia Smalla -- Proteomic Protocols for the Study of Filamentous Fungi / Raquel González Fernández, Jesús V. Jorrín Novo -- Detection and Quantification of Endoprotease Activity Using a Coomassie Dye-Binding Assay / Anthony J. O'Donoghue, Cathal S. Mahon
Protocol of a LightCycler[TM] PCR Assay for Detection and Quantification of Aspergillus fumigatus DNA in Clinical Samples of Neutropenic Patients / Birgit Spiess, Dieter Buchheidt -- Application of Polymerase Chain Reaction and PCR-Based Methods Targeting Internal Transcribed Spacer Region for Detection and Species-Level Identification of Fungi / K. Lily Therese, R. Bagyalakshmi, H.N. Madhavan -- Real-Time PCR Assay in Fungi / Naomichi Yamamoto -- Quantitative Sampling Methods for the Analysis of Fungi: Air Sampling / Mary C. O'Loughlin, Katherine D. Turner -- Transformation of Filamentous Fungi in Microtiter Plate / Bianca Gielesen, Marco van den Berg -- Molecular Fingerprinting of Fungal Communities in Soil / Roberto A. Geremia, Lucie Zinger -- Development of Microsatellite Markers from Fungal DNA Based on Shotgun Pyrosequencing / Shaobin Zhong -- Multiplex and Quantifiable Detection of Infectious Fungi Using Padlock Probes, General qPCR, and Suspension Microarray Readout / Magnus Jobs, Ronnie Eriksson, Jonas Blomberg
Rapid Deletion Plasmid Construction Methods for Protoplast and Agrobacterium-based Fungal Transformation Systems / María D. García-Pedrajas, Zahi Paz -- Improved Transformation Method for Alternaria Brassicicola and Its Applications / Yangrae Cho, Akhil Srivastava, Christopher Nguyen -- Methods for High-Quality DNA Extraction from Fungi / Vijai Kumar Gupta, Maria G. Tuohy, Rajeeva Gaur -- Production of Recombinant Proteins from Pichia pastoris: Interfacing Fermentation and Immobilized Metal Ion Affinity Chromatography / Berend Tolner, Gaurav Bhavsar, Bride Foster -- Development of a Real-Time Quantitative PCR Assay for the Assessment of Uncultured Zoosporic Fungi / Télesphore Sime-Ngando, Marlène Jobard -- Nucleic and Protein Extraction Methods for Fungal Exopolysaccharide Producers / Jochen Schmid, Dirk Mueller-Hagen, Volker Sieber -- Directed Evolution of a Fungal Xylanase for Improvement of Thermal and Alkaline Stability / Dawn Elizabeth Stephens, Suren Singh
Genome Shuffling Protocol for the Pentose-Fermenting Yeast Scheffersomyces stipitis / Paramjit K. Bajwa, Nicole K. Harner -- Detection and Identification of Fungal Microbial Volatile Organic Compounds by HS-SPME-GC-MS / Bernhard Kluger, Susanne Zeilinger -- Transformation Methods for Slow-Growing Fungi / Suman Mukherjee, Rebecca Creamer -- Enzymatic Saccharification of Lignocellulosic Biomass / Manimaran Ayyachamy, Vijai Kumar Gupta -- Protoplast Fusion Techniques in Fungi / Annie Juliet Gnanam -- Large-Scale Production of Lignocellulolytic Enzymes in Thermophilic Fungi / Manimaran Ayyachamy, Mary Shier, Maria G. Tuohy -- Panfungal PCR Method for Detection of Aflatoxigenic Molds / Malik M. Ahmad, Pravej Alam, M.Z. Abdin -- Protocols for the Quantification of dsDNA and Its Fragmentation Status in Fungi / Ioannis Papapostolou, Konstantinos Grintzalis
Rapid Identification and Detection of Pathogenic Fungi by Padlock Probes / Clement K.M. Tsui, Bin Wang, Cor D. Schoen -- Drug-Induced Permeabilization in Fungi / Maria D. Mayan, Alexandra McAleenan -- Extraction and Characterization of Taxol: An Anticancer Drug from an Endophytic and Pathogenic Fungi / M. Pandi, P. Rajapriya, P.T. Manoharan -- Identification of Mycotoxigenic Fungi Using an Oligonucleotide Microarray / Eugenia Barros -- DNA Microarray-Based Detection and Identification of Fungal Specimens / Minna Mäki -- Bioinformatic Protocols and the Knowledge-Base for Secretomes in Fungi / Gengkon Lum, Xiang Jia Min -- High-Throughput Functional Annotation and Data Mining of Fungal Genomes to Identify Therapeutic Targets / Gagan Garg, Shoba Ranganathan -- Application of Support Vector Machines in Fungal Genome and Proteome Annotation / Sonal Modak, Shimantika Sharma -- Bioinformatics Tools for the Multilocus Phylogenetic Analysis of Fungi / Devarajan Thangadurai, Jeyabalan Sangeetha
Summary "Laboratory Protocols in Fungal Biology presents the latest techniques in fungal biology. This book analyzes information derived through real experiments, and focuses on cutting edge techniques in the field. The book comprises 57 chapters contributed from internationally recognised scientists and researchers. Experts in the field have provided up-to-date protocols covering a range of frequently used methods in fungal biology. Almost all important methods available in the area of fungal biology viz. taxonomic keys in fungi; histopathological and microscopy techniques; proteomics methods; genomics methods; industrial applications and related techniques; and bioinformatics tools in fungi are covered and complied in one book. Chapters include introductions to their respective topics, list of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and notes on troubleshooting. Each chapter is self-contained and written in a style that enables the reader to progress from elementary concepts to advanced research techniques. Laboratory Protocols in Fungal Biology is a valuable tool for both beginner research workers and experienced professionals"--Publisher's description
Analysis Life sciences
Plant breeding
Plant Sciences
Plant Biochemistry
Bibliography Includes bibliographical references
Notes Online resource; title from digital title page (SpringerLink, viewed on December 31, 2014)
Subject Fungi -- Laboratory manuals.
Fungal molecular biology -- Laboratory manuals.
Molecular Biology.
Genre/Form Laboratory manuals.
Laboratory Manual.
Laboratory manuals.
Form Electronic book
Author Gupta, Vijai Kumar, editor
Tuohy, Maria G., editor
Ayyachamy, Manimaran, editor
Turner, Kevin M., editor
O'Donovan, Anthonia, editor
ISBN 9781461423560 (electronic bk.)
1461423562 (electronic bk.)
(alk. paper)
(alk. paper)