| Description |
1 online resource (815 pages) |
| Contents |
FRET -- Förster Resonance Energy Transfer: From Theory to Applications; Contents; Preface; List of Contributors; Part One: Background, Theory, and Concepts; 1 How I Remember Theodor Förster; 2 Remembering Robert Clegg and Elizabeth Jares-Erijman and Their Contributions to FRET; 2.1 Biographical Sketch of Bob Clegg; 2.2 Biographical Sketch of Eli Jares-Erijman; 2.3 The Pervasive Influence of Gregorio Weber; 2.4 Contributions by Bob Clegg to FRET; 2.5 Contributions by Eli Jares-Erijman to FRET; 2.6 A Final Thought; References; 3 Förster Theory; 3.1 Introduction; 3.2 Pre-Förster; 3.3 Bottom Line |
|
3.4 9000-Form, 9-Form, and Practical Expressions of the R6 0 Equation3.5 Overlap Integral; 3.6 Zones; 3.7 Transfer Mechanisms; 3.8 Kappa-Squared Basics; 3.9 Ideal Dipole Approximation; 3.10 Resonance as an All-or-Nothing Effect; 3.11 Details About the All-or-Nothing Approximation of Resonance; 3.12 Classical Theory Completed; 3.13 Oscillator Strength-Emission Spectrum Relation for the Donor; 3.14 Oscillator Strength-Absorption Spectrum Relation for the Acceptor; 3.15 Quantum Mechanical Theory; 3.16 Transfer in a Random System; 3.17 Details for Transfer in a Random System |
|
3.18 Concentration Depolarization3.19 FRET Theory 1965-2012; References; 4 Optimizing the Orientation Factor Kappa-Squared for More Accurate FRET Measurements; 4.1 Two-Thirds or Not Two-Thirds?; 4.2 Relevant Questions; 4.3 How to Visualize Kappa-Squared?; 4.4 Kappa-Squared Can Be Measured in At Least One Case; 4.5 Averaging Regimes; 4.6 Dynamic Averaging Regime; 4.7 What Is the Most Probable Value for Kappa-Squared in the Dynamic Regime?; 4.8 Optimistic, Conservative, and Practical Approaches; 4.9 Comparison with Experimental Results; 4.10 Smart Simulations Are Superior |
|
4.11 Static Kappa-Squared4.12 Beyond Regimes; 4.13 Conclusions; References; 5 How to Apply FRET: From Experimental Design to Data Analysis; 5.1 Introduction: FRET -- More Than a Four-Letter Word!; 5.2 FRET: Let's get started!; 5.3 FRET: The Basic Concept; 5.4 FRET: Inevitable Mathematics; 5.4.1 Förster Distance (or Förster Radius); 5.4.2 FRET Efficiency; 5.4.2.1 Determination by Donor Quenching; 5.4.2.2 Determination by Acceptor Sensitization; 5.4.2.3 Determination by Donor Quenching and Acceptor Sensitization; 5.4.2.4 Determination by Donor Photobleaching |
|
5.4.2.5 Determination by Acceptor Photobleaching5.4.3 FRET with Multiple Donors and/or Acceptors; 5.5 FRET: The Experiment; 5.5.1 The Donor-Acceptor FRET Pair; 5.5.2 Förster Distance Determination; 5.5.3 The Main FRET Experiment; 5.5.3.1 Steady-State FRET Measurements; 5.5.3.2 Time-Resolved FRET Measurements; 5.5.3.3 Interpretation of Time-Resolved FRET Data; 5.6 FRET beyond Förster; 5.6.1 Time-Resolved FRET with Lanthanide-Based Donors; 5.6.1.1 Terbium to Quantum Dot FRET Using Time-Resolved Donor Quenching and Acceptor Sensitization Analysis; 5.6.2 BRETand CRET |
| Summary |
All set to become be the ultimate reference on this growing technique, this text provides a didactic overview of the fundamentals while showing in detail how it is applied in a wide range of hot interdisciplinary applications. With its unified, well-structured approach, this is the perfect guide for both newcomers and advanced practitioners |
| Notes |
5.6.3 Energy Transfer to Metal Nanoparticles (FRET, NSET, DMPET, NPILM, etc.) |
|
Print version record |
| Subject |
Energy transfer -- Laboratory manuals
|
|
Fluorescence microscopy.
|
|
fluorescence microscopy.
|
|
NATURE -- Reference.
|
|
SCIENCE -- Life Sciences -- Biology.
|
|
SCIENCE -- Life Sciences -- General.
|
|
Energy transfer
|
|
Fluorescence microscopy
|
|
Fluoreszenz-Resonanz-Energie-Transfer
|
| Genre/Form |
Laboratory manuals
|
| Form |
Electronic book
|
| Author |
Hildebrandt, Niko
|
| ISBN |
9783527656059 |
|
3527656057 |
|
3527328165 |
|
9783527328161 |
|
9783527656042 |
|
3527656049 |
|
