| Description |
1 online resource (556 p.) |
| Contents |
Intro -- Foreword -- Preface -- Contents -- Abbreviations -- Chapter 1: General Principles -- 1.1 Flow Cytometers General Layout -- 1.2 Flow Cytometers Features -- 1.3 Parameters and Signals -- 1.4 Time -- References -- Chapter 2: Signals: Scattering -- 2.1 Forward Scatter (FSC) -- 2.2 Side Scatter (SSC) -- 2.3 Raman Scattering -- 2.4 Depolarized Scatter -- References -- Chapter 3: Signals: Fluorescence, Phosphorescence, Impedance, Extinction -- 3.1 Fluorescence -- 3.1.1 Depolarized Fluorescence -- 3.1.2 Autofluorescence -- 3.2 Phosphorescence -- 3.3 Impedance -- 3.4 Axial Extinction |
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Chapter 5: Light Sources -- 5.1 Arc Lamps -- 5.2 Lasers -- 5.2.1 Gas Lasers -- 5.2.1.1 Argon Ion Lasers -- 5.2.1.2 Krypton Ion Lasers -- 5.2.1.3 Mixed-Gas Ion Lasers (Argon/Krypton) -- 5.2.1.4 Helium-Neon Atom Lasers -- 5.2.1.5 Helium-Cadmium Ion Lasers -- 5.2.1.6 Helium-Silver and Neon-Copper Metal Vapor Lasers -- 5.2.2 Solid-State Lasers (SSLs) -- 5.2.2.1 Ultraviolet (UV) Emitting SSLs -- Deep UV -- 320 nm -- 355 nm -- 5.2.2.2 Near-Ultraviolet Emitting SSLs -- 5.2.2.3 Violet Emitting SSLs -- 5.2.2.4 Blue Emitting SSLs -- Deep Blue -- Blue-Green -- 5.2.2.5 Green and Yellow-Green Emitting SSLs |
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532 nm -- 552 nm -- 561 nm -- 5.2.2.6 Orange Emitting SSLs -- 5.2.2.7 Red Emitting SSLs -- Short Red -- Full Red -- 5.2.2.8 Infrared (IR) Emitting SSLs -- 5.2.2.9 Supercontinuum White Light Emitting SSLs -- 5.2.3 Liquid State Lasers (Dye Lasers) -- 5.3 Light Emitting Diodes (LEDs) -- References -- Chapter 6: Optical Benches -- 6.1 From the Light Source(s) to the Interrogation Point(s) -- 6.2 From the Interrogation Point(s) to the Detector(s) -- 6.3 Optical Bench Components -- 6.3.1 Absorption Filters -- 6.3.2 Interference Filters -- 6.3.3 Neutral Density Filters -- 6.3.4 Polarizing Filters |
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6.3.5 Beam Splitters -- 6.3.6 Wavelength Division Multiplexing (WDM) -- 6.3.7 Prisms, Gratings, Coarse WDM (CWDM) -- 6.4 Optical Bench Layouts -- 6.4.1 Transmission Benches -- 6.4.2 Reflection Benches -- 6.4.3 Multilaser Benches -- 6.4.3.1 Spaced Lasers and Separate Pathways/Detectors -- 6.4.3.2 Spaced Lasers and Shared Pathways/Detectors -- 6.4.3.3 Time Delay -- 6.4.3.4 Continuous Collinear Lasers -- 6.4.3.5 Pulsed Collinear Lasers -- 6.4.4 Special Solutions -- 6.4.4.1 Pie-Shaped Design -- 6.4.4.2 Benches for (De)Polarized Signals -- 6.4.4.3 Spectrally Enhanced Optical Benches -- References |
| Summary |
This book covers all the technical aspects of flow cytometry needed to set-up the instrument, solve problems encountered in daily work, or necessary for exam preparation. It provides the reader with an in-depth look at the device and its applications. Each component and its function is described in an easy-to-understand manner, giving the reader a sound basic knowledge of this instrument. The practical examples given, simplify and enhance the learning process. This book is a unique resource of knowledge for biomedical engineers and biotechnologists, flow cytometry operators, laboratory technicians and biomedical researchers, both biologists as well as medical doctors, and can also be a helpful tool for companies and manufacturers |
| Bibliography |
References -- Chapter 4: Fluidics -- 4.1 Overview on Fluids -- 4.1.1 Laminar Flow and Turbulent Flow -- 4.1.2 Hydrodynamic Focusing -- 4.2 Cytometer Fluidics -- 4.2.1 Sheath and Core -- 4.2.2 Flow System Components -- 4.2.3 Flow Rate Control -- 4.2.4 Sample Injection -- 4.2.4.1 Sample Differential Control -- 4.2.4.2 Absolute Counts -- 4.2.5 Event-Light Interaction: The Interrogation Point -- 4.2.5.1 In-Cuvette Interaction -- 4.2.5.2 Stream-in-Air Interaction -- 4.2.5.3 Interrogation on an Open Surface -- 4.2.5.4 Systems Based on Acoustic Focusing -- 4.2.5.5 Sheathless Systems -- References |
| Notes |
Chapter 7: Detectors and Electronics |
| Bibliography |
Includes bibliographical references and index |
| Notes |
Online resource; title from PDF title page (SpringerLink, viewed December 1, 2022) |
| Subject |
Flow cytometry.
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Flow cytometry
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| Form |
Electronic book
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| ISBN |
9783031108365 |
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3031108361 |
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