| Description |
1 online resource (xii, 315 pages) : illustrations (chiefly color) |
| Contents |
Intro -- Foreword -- Preface -- Contents -- Part I: General Topics -- Chapter 1: History of Flow Cytometry -- 1.1 Introduction -- 1.1.1 History of the Machines -- 1.1.1.1 Microscopy -- 1.1.1.2 The Coulter Principle -- 1.1.1.3 The Rise of Computers -- 1.1.1.4 Hydrodynamic Focusing -- 1.1.1.5 Flow Cytometers -- 1.1.1.6 Bench Top Flow Cytometers -- References -- Chapter 2: Basic Principles of Flow Cytometry -- 2.1 Introduction -- 2.2 The Flow Cytometer and How It Works -- 2.2.1 The Hydrodynamic System: How Particles Are Aligned |
|
2.2.2 Interaction of Light with Particles: Light Scatter and Fluorescence -- 2.2.3 The LASER Beam -- 2.2.4 The Optical System: How the Flow Cytometer Detects the Light Signals -- 2.2.5 The Computer System -- 2.2.6 Data Display and Analysis -- 2.2.7 Basic Flow Cytometry Procedures -- 2.2.7.1 Classical Clinical Flow Cytometry -- 2.2.7.2 DNA Analysis by Flow Cytometry -- 2.2.8 Applications of Flow Cytometry -- 2.3 Quality Control in Flow Cytometry Experiments -- 2.3.1 How Is Quality Is Assessed in Flow Cytometry? -- 2.3.2 Factors that Influence Result Quality and Recommended Corrective Actions |
|
2.3.2.1 Instrument Related Factors -- 2.3.2.2 Preanalytical Factors -- 2.3.2.3 Analytical Factors -- 2.3.2.4 Data Analysis Related Factors -- 2.4 Concluding Remarks -- References -- Chapter 3: Sample-Data Analysis -- 3.1 Introduction -- 3.1.1 Data Acquisition in the Electronics System -- 3.1.2 Storage Standard for Cytometry Files -- 3.2 Presentation of Current Techniques for Sample Analysis -- 3.2.1 A Primer on Flow-Cytometry Software -- 3.2.2 Gating a Population of Interest -- 3.2.3 One Parameter Histogram Plots: The Paradigm of DNA Content Analysis |
|
3.2.4 Two Parameter Analysis: Dot Plots and Alternative Visualization Methods -- 3.2.5 Next Generation Data Analysis of High Dimensional Data -- 3.3 Future Perspectives -- References -- Part II: Intraoperative Flow Cytometry -- Chapter 4: Basic Principles -- 4.1 Introduction -- 4.2 Basic Discussion on Intraoperative Flow Cytometry History and Applications -- 4.3 Basic Principles on Sample Size Requirements -- 4.4 Sample Acquisition, Storage, and Use -- 4.5 Conclusions -- References -- Chapter 5: Sample: Data Analysis -- 5.1 Introduction |
|
5.2 Presentation of Current Techniques for Intraoperative Sample Analysis -- 5.2.1 A Brief Summary on Sample Preparation -- 5.2.2 Pre-analytical Considerations -- 5.2.3 Flow Cytometric DNA Content Analysis -- 5.2.4 Evaluation of Results -- 5.3 Conclusions -- References -- Part III: Intraoperative Flow Cytometry in CNS Malignancies -- Chapter 6: Pathology of the Tumors of the Central Nervous System -- 6.1 Introduction -- 6.2 Gliomas -- 6.2.1 Adult-Type Diffuse Gliomas -- 6.2.1.1 Astrocytoma, IDH-Mutant -- Histopathology-Molecular Pathology |
| Summary |
This book describes intraoperative flow cytometry in solid tumours. Intraoperative flow cytometry is an innovative technique for assessing tumour margins and grade of malignancy intraoperatively. The authors have been working on this procedure for more than 14 years and have introduced it in the surgery of intracranial tumours, breast cancer, liver cancer and head and neck neoplasms, as described in the book. A detailed description of the main pathological findings will be followed by an explanation of the intraoperative flow cytometrys role. In each chapter, information will be provided to stimulate further research on this topic. The book will also include new topics that have not been published till now. The book is aimed at neurosurgeons, general surgeons, ENT specialists, breast surgeons, radiologists, pathologists, oncologists, biologists, biochemists and scientists working with on flow cytometry |
| Bibliography |
Includes bibliographical references |
| Notes |
Description based on online resource; title from digital title page (viewed on October 23, 2023) |
| Subject |
Flow cytometry -- Diagnostic use.
|
|
Cancer -- Diagnosis.
|
|
Cancer -- Diagnosis.
|
|
Flow cytometry -- Diagnostic use.
|
| Form |
Electronic book
|
| Author |
Alexiou, Georgios, editor
|
|
Vartholomatos, Georgios
|
| ISBN |
9783031335174 |
|
3031335171 |
|
