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Title Flow cytometry protocols / edited by Mark J. Jaroszeski, Ricard Heller
Published Totowa, N.J. : Humana Press, ©1998


Description 1 online resource (x, 274 pages) : illustrations
Series Methods in molecular biology ; v. 91
Methods in molecular biology (Clifton, N.J.) ; v. 91
Contents Basics of flow cytometry / Gilbert Radcliff, Mark J. Jaroszeski -- Detection of terminal transferase in leukemia / Elisabeth Paietta -- The use of flow cytometry to detect intracellular cytokine production in individual cells / Brian E. Crucian, Raymond H. Widen -- Detection of intracellular/intranuclear antigens : applications in leukemia/lymphoma analysis / Raymond H. Widen -- Detection of intracellular/intranuclear antigens in tumor cells / Raymond H. Widen, Jeanne L. Becker -- Detection of cyclins in individual cells by flow and laser scanning cytometry / Gloria Juan, Zbigniew Darzynkiewicz -- Estimation of cell viability by flow cytometry / James L. Weaver -- Flow cytometric detection of fluorescent redistributional dyes for measurement of cell transmembrane potential / Michael K. Tanner, Samuel R. Wellhausen -- Flow cytometric analysis of macrophage oxidative metabolism using DCFH / Amy Imrich, Lester Kobzik -- Measurement of environmental particulate uptake by lung cells using flow cytometry / Bradley Stringer, Lester Kobzik -- Estimation of drug resistance by flow cytometry / Adorjan Aszalos, James L. Weaver -- Flow cytometric monitoring of cellular pharmacokinetics / Margaret S. Dordal -- Quantification of xenoprotein electroinsertion in mammalian cells / Khalid Ouagari, Justin Teissié -- Flow cytometry quantification of electropermeabilization / Marie-Pierre Rols, Justin Teissié -- Flow cytometric detection and quantitation of cell-cell electrofusion products / Mark J. Jaroszeski, Richard Gilbert, Richard Heller -- Cell cycle analysis of asynchronous populations / Michael G. Ormerod -- Simultaneous DNA/RNA analysis of solid and hematoreticular malignancies / Kim Steck, Adel El-Naggar -- Solid tumor DNA ploidy analysis / Kim Steck, Adel El-Naggar -- FISHing for cytokines : methodology combining flow cytometry and in situ hybridization / Kenneth J. Pennline -- Analysis of apoptosis by flow cytometry / Wojclech Gorczyca, Myron R. Melamed, Zbigniew Darzynkiewicz -- Chromosome sorting and analysis by FACS / Simon P. Monard -- Simultaneous five-six color multiparameter analysis / Kenneth J. Pennline
Summary In Flow Cytometry Protocols, leading researchers and experimenters update the complex and technically sophisticated field of flow cytometry with detailed accounts of their many new, improved, and well-tested protocols and applications. Among the cutting-edge methods presented here are techniques for the detection of terminal transferase ion leukemia, intracellular cytokines, intracellular/intranuclear antigens in leukemia and tumors, and cyclins. Protocols for performing functional studies include methods for assessing viability, transmembrane potential, oxidative metabolism, environmental particulate uptake, drug resistance, and cellular pharmacokinetics. The methods for examining DNA address cell cycle analysis, DNA/RNA analysis of solid malignancies, solid-tumor DNA ploidy analysis, apoptosis, and a method that combines FISH with flow cytometry. Advanced methods for chromosome sorting and 5-6 color analysis are also detailed. Flow Cytometry Protocols contains methods that take advantage of the recently developed instrumentation and the vast array of novel probes that have led to advances in the field of flow cytometry, as well as to significantly improved standard methods. This eminently practical new book will play a key role in bringing researchers up-to-date in this unique and productive technology
Analysis doorstroomcytometrie
flow cytometry
biologische technieken
biological techniques
Biological Techniques
Biologische technieken
Bibliography Includes bibliographical references and index
Notes English
Print version record
Subject Flow cytometry -- Laboratory manuals
Flow Cytometry -- methods
MEDICAL -- Histology.
Flow cytometry
Genre/Form Laboratory manuals
Laboratory manuals.
Manuels de laboratoire.
Form Electronic book
Author Jaroszeski, Mark J.
Heller, Richard, 1954-
ISBN 058524619X