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Title Cellular diagnostics : basic principles, methods and clinical applications of flow cytometry / editors, Ulrich Sack, Attila Tárnok, Gregor Rothe
Published Basel ; New York : Karger, 2009


Description 1 online resource (xxx, 738 pages) : illustrations (some color)
Contents ""Cover""; ""Contents""; ""Background and Methodological Principles""; ""Characterization and Phenotyping of Cells""; ""Analysis of Cell Functions""; ""Diagnostic Indications""; ""Authors""; ""Abbreviations""; ""Wallace Henry Coulter � a Short Biography""; ""Wallace�s Far East Adventures""; ""An Elegant Idea Becomes a Company""; ""On the Personal Side""; ""Foreword""; ""Preface""; ""Background and Methodological Principles""; ""Cytometry � a Definitive History of the Early Days""; ""Prelude""; ""Introduction""; ""The Beginning""; ""The Beginning of Cytometry""
""Wallace Coulter � Inventor and Entrepreneur""""Invention of the Electrostatic Cell Sorter""; ""The Other Side of Flow""; ""Background on Fulwyler�s Invention""; ""The Parallel Discoveries in Europe""; ""Leeonard Herzenberg and the Stanford Group""; ""Particle Technologies and the Integration into Coulter Electronics""; ""1, 2, 3, 4 ... n Colors: Cytometry Gets Complex""; ""Standards: Who Needs Them Anyway?""; ""Calibration: The Promise of Quantitative Fluorescence""; ""The Emergence of Clinical Cytometry""; ""The Reemergence of Cell Sorting""; ""The Future: The Next Generation""
PostludeAcknowledgments -- References -- Concept Developments in Flow Cytometry -- Introduction -- Cytophotometry -- Flow Technology -- Impulse Cytophotometry -- Nomenclature -- Clinical Impulse Cytophotometry -- Further Instrument Developments -- Experimental and Clinical DNA Cytometry -- Chromosome Analysis, FISH -- DNA Cell Cycle Analysis, Micronuclei, Hematopoietic Stem Cells -- Predictive Cytology and Cytopathology by DNA Image Cytometry -- Immunophenotyping -- Fluorescence Anisotropy, FRET, Polarized Light and Raman Scatter
Measurement of Cell FunctionsApoptosis -- Microbiology and Biotechnology -- Data Analysis -- Predictive Medicine by Cytomics -- Outlook -- Summary -- Acknowledgments -- References -- Technical Background and Methodological Principles of Flow Cytometry -- Introduction -- Technical Background -- Fluorescent Detection of Biomarkers -- Basic Methods of Cell Preparation -- Conclusion -- References -- Technical and Methodological Basics of Slide-Based Cytometry -- Introduction/Background -- Laser Scanning Cytometry (Photomultiplier-Generated Images)
Cytometric Analyses Using Fluorescence Microscopy (Camera-Based Images)Principal Considerations for Slide-Based Cytometry -- Sample Preparations for Slide-Based Cytometry -- Statement -- References -- Selection and Combination of Fluorescent Dyes -- Introduction -- Basics -- Excitation of Fluorescent Dyes -- Fluorescent Dyes Suitable for Immune Staining -- Live/Dead Dyes -- In-House Conjugation of Reagents -- Principles for the Selection of Fluorescent Dyes -- Amplification Techniques -- Background Signal -- Summary -- Interesting Web Sites and Literature
Summary This book is the updated English version of the 2006 German bestseller 'Zelluläre Diagnostik', a comprehensive presentation of flow cytometry and its applications. While some techniques of immunophenotyping by flow cytometry already are routine procedures in the laboratory, new methods for the functional characterization of cells, the analysis of rare cells, and the diagnosis of complex materials have only begun to win wide recognition. New approaches such as slide-based cytometry will lead to an increase in the use of cytometric techniques. Multiparameter approaches will further improve analysis. The book provides a comprehensive and detailed compilation of all aspects of flow cytometry in research and the clinic. For newcomers it offers a thorough introduction, for advanced users, specific protocols and interpretation assistance
Notes "This book is the updated English version of ... Zelluläre Diagnostik"--Page 4 of cover
Bibliography Includes bibliographical references and index
Notes Print version record
Subject Flow cytometry -- Diagnostic use.
Cell physiology.
Flow Cytometry -- methods
Cell Physiological Phenomena
Diagnostic Techniques and Procedures
Fluorescence Resonance Energy Transfer -- methods
Immunophenotyping -- methods
Flow cytometry -- Diagnostic use.
Durchflusscytometrie -- Aufsatzsammlung.
Form Electronic book
Author Sack, U. (Ulrich)
Tárnok, Attila
Rothe, Gregor
S. Karger (Firm)
ISBN 9783805585569
OTHER TI Zelluläre Diagnostik. English