| Description |
1 online resource (xv, 530 pages) : illustrations (some color) |
| Series |
Methods in molecular biology, 1940-6029 ; volume 1627 |
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Methods in molecular biology (Clifton, N.J.) ; v. 1627. 1064-3745
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| Contents |
880-01 Ullrich, and Scott N. Byrne 15. Cell-Populated Collagen Lattice Models Beate Eckes, Fang Wang, Laure Rittié, Gabriele Scherr, and Paola Zigrino 16. Traction Force Measurement using Deformable Microposts Tianfa Xie, Jamar Hawkins, and Yubing Sun 17. Mechanical Deformation of Cultured Cells with Hydrogels Christal A. Worthen, Laure Rittié, and Gary J. Fisher 18. Preparation of Decellularized Lung Matrices for Cell Culture and Protein Analysis Franziska E. Uhl, Darcy E. Wagner, and Daniel J. Weiss Part IV: ECM Proteins |
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880-01/(S Human fibrotic diseases : current challenges in fibrosis research / Joel Rosenbloom, Edward Macarak, Sonsoles Piera-Velazquez, and Sergio A. Jimenez -- Bleomycin model of pulmonary fibrosis / Tianju Liu, Francina Gonzalez De Los Santos, and Sem H. Phan -- Intradermal injections of bleomycin to model skin fibrosis / Toshiyuki Yamamoto -- Assessing the effects of fibrosis on lung function by light microscopy-coupled stereology / Bartosz Pilecki and Grith Lykke Sørensen -- Transplanting human skin grafts onto nude mice to model skin scars / Jie Ding and Edward E. Tredget -- Hypertrophic scarring in the rabbit ear : a practical model for studying dermal fibrosis / Layla Nabai and Aziz Ghahary -- Mouse and rat models of induction of hepatic fibrosis and assessment of portal hypertension / Sabine Klein, Robert Schierwagen, Frank Erhard Uschner, and Jonel Trebicka -- Mouse models of corneal scarring / Laure Rittié, Audrey E.K. Hutcheon, and James D. Zieske -- Modeling cardiac fibrosis in mice : (myo)fibroblast phenotype after ischemia / Daniela Fraccarollo, Paolo Galuppo, and Johann Bauersachs -- Characterization of mesenchymal-fibroblast cells using the Col1a2 promoter/enhancer / Ian M.H. Li, Amy L. Horwell, Grace Chu, Benoit de Crombrugghe, and George Bou-Gharios -- Isolation and culture of primary murine hepatic stellate cells / Sabine Weiskirchen, Carmen G. Tag, Sibille Sauer-Lehnen, Frank Tacke, and Ralf Weiskirchen -- Isolation and culture of adipose-derived stromal cells from subcutaneous fat / Lawrence J. Liew, Huan Ting Ong, and Rodney J. Dilley -- Isolation of live fibroblasts by fluorescence-activated cell sorting / Tripp Leavitt, Michael S. Hu, and Michael T. Longaker -- Detection of infiltrating mast cells using a modified toluidine blue staining / Nahum Puebla-Osorio, Seri N.E. Sarchio, Stephen E. Ullrich, and Scott N. Byrne -- Cell-populated collagen lattice models / Beate Eckes, Fang Wang, Laure Rittié, Gabriele Scherr, and Paola Zigrino -- Traction force measurement using deformable microposts / Tianfa Xie, Jamar Hawkins, and Yubing Sun -- Mechanical deformation of cultured cells with hydrogels / Christal A. Worthen, Laure Rittié, and Gary J. Fisher -- Preparation of decellularized lung matrices for cell culture and protein analysis / Franziska E. Uhl, Darcy E. Wagner, and Daniel J. Weiss -- Type I collagen purification from rat tail tendons / Laure Rittié -- Purification of human plasma/cellular fibronectin and fibronectin fragments / Giampiero Pietrocola, Simonetta Rindi, Giulia Nobile, and Pietro Speziale -- Laser capture microdissection of tissue sections for high-throughput RNA analysis / Julia Hoffmann, Jochen Wilhelm, and Grazyna Kwapiszewska -- Collagen quantification in tissue specimens / João Quintas Coentro, Héctor Capella-Monsonís, Valeria Graceffa, Zhuning Wu, Anne Maria Mullen, Michael Raghunath, and Dimitrios I. Zeugolis -- Methods for the assessment of active transforming growth factor-β in cells and tissues / Alison E. John, Joanne Porte, Gisli Jenkins, and Amanda L. Tatler -- Visualizing in vitro type I collagen fibrillogenesis by transmission electron microscopy / J. Robin Harris -- Histological and electron microscope staining for the identification of elastic fiber networks / Elaine C. Davis and Ling Li -- Method for picrosirius red-polarization detection of collagen fibers in tissue sections / Laure Rittié -- Probing collagen organization : practical guide for second-harmonic generation (SHG) imaging / Riccardo Cicchi and Francesco S. Pavone -- Methods for quantifying fibrillar collagen alignment / Yuming Liu, Adib Keikhosravi, Guneet S. Mehta, Cole R. Drifka, and Kevin W. Eliceiri -- Exploring the nano-surface of collagenous and other fibrotic tissues with AFM / Andreas Stylianou, Vasiliki Gkretsi, Costas S. Patrickios, and Triantafyllos Stylianopoulos -- Spectral unmixing methods and tools for the detection and quantitation of collagen and other macromolecules in tissue specimens / Zachary T. Harmany, Farzad Fereidouni, and Richard M. Levenson -- Simple analysis of deposited gene expression datasets for the non-bioinformatician : how to use GEO for fibrosis research / Yang Guo, Richard Townsend, and Lam C. Tsoi |
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Method for Picrosirius Red-Polarization Detection of Collagen Fibers in Tissue Sections Laure Rittié 27. Probing Collagen Organization: Practical Guide for Second Harmonic Generation (SHG) Imaging Riccardo Cicchi and Francesco S. Pavone Part V: ECM Proteins -- Purification, Quantification, and Analysis 28. Methods for Quantifying Fibrillar Collagen Alignment Yuming Liu, Adib Keikhosravi, Guneet S. Mehta, Cole R. Drifka, and Kevin W. Eliceiri 29. Exploring the Nano-Surface of Collagenous and Other Fibrotic Tissues with AFM Andreas Stylianou, Vasiliki Gkretsi, Costas S. Patrickios, and Trianatafyllos Stylianopoulos 30. Spectral Unmixing Methods and Tools for the Detection and Quantitation of Collagen and Other Macromolecules in Tissue Specimens Zachary T. Harmany, Farzad Fereidouni, and Richard M. Levenson 31. Simple Analysis of Deposited Gene Expression Datasets for the Non-Bioinformatician: How to Use GEO for Fibrosis Research Yang Guo, Richard Townsend, and Lam C. Tsoi |
| Summary |
This volume describes state-of-the-art protocols that serve as "recipes" for scientists concentrating on fibrosis research. This book is divided into four sections. Part I focuses on animal models of fibrosis and covers topics such as mimicking fibrosis in the lungs, skin, liver and heart, and generating transgenic mouse models. Part II discusses cell culture systems, where the chapters explore cell types important for the development of fibrosis. Part III looks at the purification, quantification, and analysis of the ECM proteins, and Part IV describes computer-assisted methods such as quantifying fibrillar collagen alignment and exploring the nano-surface of collagen with atomic force microscopy (AFM). Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Cutting-edge and practical, Fibrosis: Methods and Protocols is a valuable resource aimed at outstanding quality and repeatability of research experiments in the fibrosis field |
| Bibliography |
Includes bibliographical references and index |
| Notes |
Online resource; title from PDF title page (SpringerProtocol, viewed August 24, 2017) |
| Subject |
Fibrosis -- Laboratory manuals
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Fibrosis.
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Fibrosis
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Medical research.
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Science -- Life Sciences -- Biology -- Molecular Biology.
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Fibrosis
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| Genre/Form |
Laboratory manuals
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Laboratory manuals.
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Manuels de laboratoire.
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| Form |
Electronic book
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| Author |
Rittié, Laure, editor.
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| ISBN |
9781493971138 |
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1493971131 |
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