Title Extracellular matrix protocols / edited by Charles H. Streuli and Michael E. Grant

Published Totowa, N.J. : Humana Press, 2000

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Description 1 online resource (xiv, 370 pages) : illustrations Series Methods in molecular biology ; v. 139 Methods in molecular biology (Clifton, N.J.) ; v. 139. Contents Semipermeabilized cells to study procollagen assembly / Richard R. Wilson, Neil J. Bulleid -- Quantitative determination of collagen crosslinks / Trevor J. Sims, Nicholas C. Avery, Allen J. Bailey -- Analysis of laminin structure and function with recombinant glycoprotein expressed in insect cells / Todd L. Mathus, Peter D. Yurchenco -- Recombinant collagen trimers from insect cells and yeast / Johanna Myllyharju -- Eukaryotic expression and purification of recombinant extracellular matrix proteins carrying the Strep II tag / Neil Smyth [and others] -- Preparation of isotopically labeled recombinant fragments of fibronectin for functional and structural study by heteronuclear nuclear magnetic resonance spectroscopy / Jeremy R. Bright [and others] -- Reconstitution of functional integrin into phospholipid vesicles and planar lipid bilayers / Eva-Maria Erb, Jürgen Engel -- Confocal-FRAP analysis of ECM molecular interactions / Timothy Hardingham, Philip Gribbon -- Electron cryomicroscopy of fibrillar collagens / Roger S. Meadows [and others] -- Atomic force microscopy measurements of intermolecular binding strength / Gradimir N. Misevic -- ECM macromolecules : rotary shadowing and scanning transmission electron microscopy / Michael J. Sherratt [and others] -- Screening for mutations in cartilage ECM genes / Michael D. Briggs -- Tissue-specific KO of ECM proteins / Emilio Hirsch, Mara Brancaccio, Fiorella Altruda -- Homologous gene targeting to study ECM assembly / Francesco Ramirez, Friedrich Laub, Hideaki Sumiyoshi -- Enhancer analysis of the [alpha]1(II) and [alpha]2(XI) collagen genes in transfected chondrocytes and transgenic mice / Noriyuki Tsumaki [and others] -- Retroviral delivery of ECM genes to cells / Kyonggeun Yoon, Vitali Alexeev -- Using self-assembled monolayers to pattern ECM proteins and cells on substrates / Christopher S. Chen [and others] -- Methods for preparing extracellular matrix and quantifying insulin-like growth factor-binding protein binding to the ECM / Bo Zheng, David R. Clemmons -- Measuring interactions between ECM and TGF[beta]-like proteins / Sarah L. Dallas -- Using organotypic tissue slices as substrata for the culture of dissociated cells / Daniel E. Emerling, Arthur D. Lander -- Neuroepithelial differentiation induced by ECM molecules / José María Frade, Alfredo Rodriguez Tébar -- Migration assays for oligodendrocyte precursor cells / Emma E. Frost, Richard Milner, Charles ffrench-Constant -- Cell adhesion assays / Martin J. Humphries -- Tissue engineering and cell-populated collagen matrices / Paul D. Kemp -- Solid phase assays for studying ECM protein-protein interactions / A. Paul Mould -- Tissue engineering of cartilage / Ronda E. Schreiber, Anthony Ratcliffe -- Tissue recombinants to study extracellular matrix targeting to basement membranes / Patricia Simon-Assmann, Michèle Kedinger -- Fluorescence assays to study cell adhesion and migration in vitro / Paola Spessotto, Emiliana Giacomello, Roberto Perris -- Analyzing cell-ECM interactions in adult mammary gland by transplantation of embryonic mammary tissue from knockout mice / Teresa C.M. Klinowska, Charles H. Streuli Summary It is now widely accepted that much of the dynamic function of cells and tissues is regulated from outside the cell by the extracellular matrix (ECM). In Extracellular Matrix Protocols, leading bench-top experimentalists from around the world present in a proven format their state-of-the-art methods for probing the extracellular matrix, its mechanisms of assembly, and how it influences cell behavior. These carefully detailed biochemical, biophysical, molecular biological, and cell biological techniques have been optimized for the study ECM structure and assembly, the mechanisms of cell-ECM interactions, and the molecular genetics of ECM proteins and defects in human disease, and bring to a wider audience a range of highly sophisticated methods that have hitherto been difficult even for a few specialized laboratories. Cutting-edge and highly practical, Extracellular Matrix Protocols offers today's ECM researchers a gold-standard collection of powerful, readily reproducible methods to investigate the extracellular matrix and the altered cell-ECM interactions that play so important a role in human health and disease Bibliography Includes bibliographical references and index Notes English Print version record Subject Extracellular matrix -- Laboratory manuals Extracellular matrix proteins -- Laboratory manuals Extracellular matrix. Extracellular Matrix Extracellular Matrix Proteins SCIENCE -- Life Sciences -- Cell Biology. Extracellular matrix Extracellular matrix proteins Genre/Form Laboratory manuals Laboratory manuals. Manuels de laboratoire. Form Electronic book Author Streuli, Charles H Grant, Michael E ISBN 0896036243 9780896036246 0585356068 9780585356068 1592590632 9781592590636 1280820543 9781280820540 9786610820542 6610820546 9780896036345 0896036340

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