| Description |
1 online resource |
| Contents |
Front Cover; Fluorescence Microscopy: Super-Resolution and Other Novel Techniques; Copyright; Contents; Preface; List of Contributors; Chapter 1 -- Evanescent Excitation and Emission; INTRODUCTION; EVANESCENCE IN GENERAL; EVANESCENCE IN EXCITATION: TIR; EVANESCENCE IN EMISSION: THE FLUOROPHORE NEAR FIELD; EVANESCENCE FROM A POINT SOURCE: NSOM; Acknowledgments; References; Chapter 2 -- Adaptive Optics for Fluorescence Microscopy; INTRODUCTION; ABERRATIONS IN MICROSCOPY; PRINCIPLES OF ADAPTIVE OPTICS; ADAPTIVE ABERRATION CORRECTION IN FLUORESCENCE MICROSCOPES; WAVEFRONT SENSING |
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SCHEMES FOR MODAL SENSORLESS ADAPTIVE OPTICSCONCLUSION; References; Chapter 3 -- Rapid Measurements of Orientation and Rotation in Ex Vivo Muscle Made Possible by Studying Small Number of Cross-Bridges; INTRODUCTION; KINETICS; DISTRIBUTION OF CROSS-BRIDGE ORIENTATIONS; CONCLUSIONS; Acknowledgments; References; Chapter 4 -- High Spatiotemporal Bioimaging Techniques to Study the Plasma Membrane Nanoscale Organization; INTRODUCTION; SPATIAL RESOLUTION; TEMPORAL RESOLUTION; CONCLUSIONS AND OUTLOOK; References; Chapter 5 -- High-Resolution 3D Imaging of Intact Transparent Organs by 3DISCO |
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INTRODUCTIONCURRENT TECHNOLOGIES FOR IMAGING THE INTACT BRAIN; 3DISCO; Acknowledgments; References; Chapter 6 -- Using RNA Mimics of GFP to Image RNA Dynamics in Mammalian Cells; INTRODUCTION; STUDYING TRINUCLEOTIDE-REPEAT-CONTAINING RNA; OPTIMIZING IMAGING OF CGG REPEAT-CONTAINING RNA; TIME-LAPSE IMAGING OF CGG-REPEAT-CONTAINING RNAS; MONITORING CGG REPEAT FOCI DURING CELL DIVISION; EXAMINING THE STABILITY OF CGG60 RNA; IMAGING THE EFFECTS OF SMALL-MOLECULE DRUGS ON CGG FOCI; IMAGING OTHER RNAS USING SPINACH2; TROUBLESHOOTING IMAGING WITH SPINACH2-TAGGED CONSTRUCTS; IMAGING RARE RNAS |
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FUTURE DIRECTIONSReferences; Chapter 7 -- Surface Enhanced Raman Scattering (SERS) Image Cytometry for High-Content Screening; HIGH-CONTENT SCREENING: STATUS AND NEEDS; LABELS AND PROBES FOR HCS: FLUORESCENCE AND SERS; SPECTRAL IMAGING FOR FLUORESCENCE AND SERS; SERS IMAGING IN CELL-BASED ASSAYS; STATUS AND PROSPECTS; Acknowledgments; References; APPENDIX; Chapter 8 -- Light Sheet Fluorescence Microscopy Applications for Multicellular Systems; INTRODUCTION; LSFM IMPLEMENTATIONS: A UNIVERSAL CONCEPT WITH MANY ACRONYMS; LSFM AT THE MACROSCOPIC SCALE; 3D CANCER IMAGING MODEL: MCTS |
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CALCIUM IMAGING, FAST VOLUMETRIC CALCIUM IMAGINGCONCLUSIONS; Acknowledgments; References; Chapter 9 -- Correlative Light Electron Microscopy as a Navigating Tool for Cryo-Electron Tomography Analysis; INTRODUCTION; ELECTRON MICROSCOPY IN CELLULAR AND STRUCTURAL BIOLOGY; SAMPLE PREPARATION FOR ELECTRON MICROSCOPY; CRYO-ELECTRON TOMOGRAPHY; CORRELATIVE LIGHT AND ELECTRON MICROSCOPY (CLEM); CORRELATED LIGHT AND ELECTRON MICROSCOPY DIRECT OBSERVATION IN CRYOGENIC TEMPERATURES; CLEM PROSPECTS; Acknowledgments; References |
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Chapter 10 -- Cellular and Molecular Applications of Super-resolution Microscopy |
| Summary |
Fluorescence Microscopy: Super-Resolution and other Novel Techniques delivers a comprehensive review of current advances in fluorescence microscopy methods as applied to biological and biomedical science. With contributions selected for clarity, utility, and reproducibility, the work provides practical tools for investigating these ground-breaking developments. Emphasizing super-resolution techniques, light sheet microscopy, sample preparation, new labels, and analysis techniques, this work keeps pace with the innovative technical advances that are increasingly vital to biological an |
| Notes |
Includes index |
| Bibliography |
Includes bibliographical references and index |
| Notes |
Text in English |
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Print version record |
| Subject |
Fluorescence microscopy.
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Microscopy, Fluorescence
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fluorescence microscopy.
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SCIENCE -- General.
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Fluorescence microscopy
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Fluoreszenzmikroskopie
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Espectroscopía de fluorescencia.
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| Form |
Electronic book
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| Author |
Cornea, Anda, editor
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Conn, P. Michael, editor
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| ISBN |
9780124167131 |
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0124167136 |
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1306464358 |
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9781306464352 |
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