Title E. coli gene expression protocols / edited by Peter E. Vaillancourt

Published Totawa, N.J. : Humana Press, ©2003

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Description 1 online resource (xi, 347 pages) : illustrations Series Methods in molecular biology ; v. 205 Methods in molecular biology (Clifton, N.J.) ; v. 205 Contents 1. Cold-Inducible Promoters for Heterologous Protein Expression / Francois Baneyx and Mirna Mujacic -- 2. Dual-Expression Vectors for Efficient Protein Expression in Both E. coil and Mammalian Cells / Rebecca L. Mullinax [and others] -- 3. A Dual-Expression Vector Allowing Expression in E: coli and P. pastoris, Including New Modifications / Angelika Lueking [and others] -- 4. Purification of Recombinant Proteins from E. coli by Engineered Inteins / Ming-Qun Xu and Thomas C. Evans, Jr. -- 5. Calmodulin as an Affinity Purification Tag / Samu Melkko and Dario Neri -- 6. Calmodulin-Binding Peptide as a Removable Affinity Tag for Protein Purification / Wolfgang Klein -- 7. Maltose-Binding Protein as a Solubility Enhancer / Jeffrey D. Fox and David S. Waugh -- 8. Thioredoxin and Related Proteins as Multifunctional Fusion Tags for Soluble Expression in E. coli / Edward R. LaVallie [and others] -- 9. Discovery of New Fusion Protein Systems Designed to Enhance Solubility in E. coli / Gregory D. Davis and Roger G. Harrison -- 10. Assessment of Protein Folding/Solubility in Live Cells / Rhesa D. Stidham [and others] -- 11. Improving Heterologous Protein Folding via Molecular Chaperone and Foldase co-Expression / Francois Baneyx and Joanne L. Palumbo -- 12. High-Throughput Purification of PolyHis-Tagged Recombinant Fusion Proteins / Thomas Lanio, Albert Jeltsch, and Alfred Pingoud -- 13. Co-Expression of Proteins in E. coil Using Dual Expression Vectors / Karen Johnston and Ronen Marmorstein -- 14. Small-Molecule Affinity-Based Matrices for Rapid Protein Purification / Karin A. Hughes and Jean P. Wiley -- 15. Use of tRNA-Supplemented Host Strains for Expression of Heterologous Genes in E. coli / Carsten-Peter Carstens -- 16. Screening Peptide/Protein Libraries Fused to the X Repressor DNA-Binding Domain in E. coil Cells / Leonardo Marino-Ramirez, Lisa Campbell, and James C. Hu -- 17. Studying Protein-Protein Interactions Using a Bacterial Two-Hybrid System / Simon L. Dove -- 18. Using Bio-Panning of FLITRX Peptide Libraries Displayed on E. coli Cell Surface to Study Protein-Protein Interactions / Zhijian Lu, Edward R. LaVallie, and John M. McCoy -- 19. Use of Inteins for the In Vivo Production of Stable Cyclic Peptide Libraries in E. coli / Ernesto Abel-Santos, Charles P. Scott, and Stephen J. Benkovic -- 20. Hyperphage : Improving Antibody Presentation in Phage Display / Olaf Broders, Frank Breitling, and Stefan Dibel -- 21. Combinatorial Biosynthesis of Novel Carotenoids in E. coli / Gerhard Sandmann -- 22. Using Transcriptional-Based Systems for In Vivo Enzyme Screening / Steven M. Firestine, Frank Salinas, and Stephen J. Benkovic -- 23. Identification of Genes Encoding Secreted Proteins Using Mini-OphoA Mutagenesis / Mary N. Burtnick, Paul J. Brett, and Donald E. Woods Summary Gene expression using E. coli as a host is carried out over a wide range of disciplines in academic and industrial laboratories. In E. coli Gene Expression Protocols, Peter E. Vaillancourt presents a collection of popular and emerging methodologies that take advantage of E. coli's ability to quickly and inexpensively express recombinant proteins. The authors focus on two areas of interest: the use of E. coli vectors and strains for production of pure, functional protein, and the use of E. coli as host for the functional screening of large collections of proteins and peptides. Among the cutting-edge techniques demonstrated are those for rapid high-level expression and purification of soluble and functional recombinant protein, and those essential to functional genomics, proteomics, and protein engineering. Described in step-by-step detail to ensure robust, trouble-free results, each proven method has been written by a hands-on expert and includes extensive notes and practical tips for avoiding pitfalls. Even highly skilled researchers will find many time-saving techniques. Authoritative and highly practical, E. coli Gene Expression Protocols provides a state-of-the-art collection of tested methods for this powerful gene expression technology, offering today's investigators proven tools for success in the emerging fields of functional genomics and proteomics Bibliography Includes bibliographical references and index Notes English In Springer Protocols Subject Gene expression -- Laboratory manuals Escherichia coli -- Genetics -- Laboratory manuals Gene expression. Escherichia coli -- genetics Gene Expression SCIENCE -- Life Sciences -- Genetics & Genomics. Escherichia coli -- Genetics Gene expression Genre/Form Laboratory manuals Laboratory manuals. Manuels de laboratoire. Form Electronic book Author Vaillancourt, Peter E ISBN 9781592593019 1592593011 1588290085 9781588290083 1280841885 9781280841880 9786610841882 6610841888

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