Silane-modified surfaces for biomaterial immobilization -- Avidin-biotin immobilization systems -- Antibiodies as immobilization reagents -- Bioanalytical applications of self-assembled monolayers -- Protein adsorption: friend or foe? -- Micropatterning cell adhesiveness -- Sol-gel matrices for protein entrapment -- Immobilization of 'smart' polymer-protein conjugates -- Kinetic analysis of the interaction between an analyte in solution and an immobilized protein -- Spectroscopic characterization of immobilized proteins -- Immobilization using electrogenerated polymers
Summary
"This book describes in detail the key methods for immobilization of biomolecules and cells on a variety of surfaces with different geometries and chemistries so that they retain their function. Full guidelines are provided on which method to use. Also included are the analytical techniques needed to measure the functionality of immobilized biomolecules. All the protocols have been tried and validated by the authors who also give invaluable hints and tips for success borne out of their own considerable practical experience." "Immobilized Biomolecules in Analysis: A Practical Approach is a 'must-have' purchase for all researchers in the fields of biosensors and biomaterials."--Jacket
