Title Cell-cell interactions : methods and protocols / edited by Sean P. Colgan

Published Totowa, N.J. : Humana Press, ©2006

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Description 1 online resource (xiii, 286 pages) : illustrations (some color) Series Methods in molecular biology, 1064-3745 ; 341 Methods in molecular biology (Clifton, N.J.) ; v. 341 Contents Blood-brain barrier and cell-cell interactions: methods for establishing in vitro models of the blood-brain barrier and transport measurements / Michael Aschner [and others] -- Interactions of intestinal epithelial cells with bacteria and immune cells: methods to characterize microflora and functional consequences / Geraldine Canny, Alexander Swidsinski, and Beth A. McCormick The role of junctional adhesion molecules in interactions between vascular cells / Triantafyllos Chavakis and Valeria Orlova -- Cell-cell interactions on solid matrices / Nancy A. Louis, Dionne Daniels, and Sean P. Colgan -- Cell-cell interactions in the kidney: inducible expression of mutant G protein [alpha]-subunits in Madin-Darby canine kidney cells for studies of epithelial cell junction structure and function / Ernesto Sabath and Bradley M. Denker -- Nucleotide metabolism and cell-cell interactions / Holger K. Eltzschig [and others] Analysis of mammalian sperm-egg membrane interactions during in vitro fertilization / Genevieve B. Wortzman, Allison J. Gardner, and Janice P. Evans -- Collagen gel contraction assay / Peter Ngo [and others] -- Methods to assess tissue permeability / Juan C. Ibla and Joseph Khoury -- Bacterial-bacterial cell interactions in biofilms: detection of polysaccharide intercellular adhesins by blotting and confocal microscopy / Kimberly K. Jefferson and Nuno Cerca -- A biochemical method for tracking cholera toxin transport from plasma membrane to golgi and endoplasmic reticulum / Heidi E. De Luca and Wayne I. Lencer -- Isolation and culture of murine heart and lung endothelial cells for in vitro model systems / Yaw-Chyn Lim and Frances W. Luscinskas Expression cloning of signaling proteins regulated by cell adhesion / Michelle L. Matter and Joe W. Ramos -- Assays for the calcium sensitivity of desmosomes / Anita J. Merritt, Anthea Scothern, and Tanusree Bhattacharyya -- Tight junctions and cell-cell interactions / Markus Utech, Matthias Brüwer, and Asma Nusrat -- Simultaneous immunofluorescence detection of cadherin and [beta]-catenin in mouse tissue sections / Nobuko Obara In vitro neutrophil transepithelial migration / Winston Y. Lee [and others] -- The blot rolling assay: a method for identifying adhesion molecules mediating binding under shear conditions / Robert Sackstein and Robert Fuhlbrigge -- Cell-cell interaction in the transcellular biosynthesis of novel [omega]-3-derived lipid mediators / Nan Chiang and Charles N. Serhan -- Isolation and analysis of lipid rafts in cell-cell interactions / Aimee Landry and Ramnik Xavier Summary The use of directed cell models to study the manner in which two or more cells interact has revolutionized our understanding of protein-protein interactions in a complex milieu and has provided an invaluable platform for the identification of new molecules. In Cell-Cell Interactions: Methods and Protocols, highly skilled experts in the development and utilization of cell-cell interaction assays describe in detail their best methods-based approaches, both in vitro and in vivo, for uncovering cellular interactions at the molecular level. The protocols cover a diverse set of cell-cell interaction models in both normal and pathological states, are readily adaptable to nearly any cell type and organ system, and include primary data and outcome analysis. In addition, the protocols follow the successful Methods in Molecular Biology series format, each offering step-by-step laboratory instructions, an introduction outlining the principles behind the technique, lists of the necessary equipment and reagents, and tips on troubleshooting and avoiding known pitfalls. Insightful to beginners and inspiring to seasoned professionals, Cell-Cell Interactions: Methods and Protocols provides the cutting-edge and versatile laboratory tools needed to gain a basic understanding of cell-cell interactions Bibliography Includes bibliographical references and index Notes English Print version record Subject Cell interaction. Cell junctions. Epithelial cells. Cell physiology. Life sciences. Cells. Anatomy. Physical sciences. Cell membranes. Physiology. Endothelial cells. Cell Communication -- physiology Endothelial Cells -- physiology Epithelial Cells -- physiology Tight Junctions -- physiology Cell Physiological Phenomena Intercellular Junctions Biological Science Disciplines Cells Anatomy Natural Science Disciplines Cell Membrane Structures Phenomena and Processes Cell Membrane Disciplines and Occupations Cellular Structures Physiology Tight Junctions Epithelial Cells Cell Communication Endothelial Cells biological sciences. anatomy. physical sciences. physiology. SCIENCE -- Life Sciences -- Cell Biology. Physiology Physical sciences Life sciences Endothelial cells Cells Cell physiology Cell membranes Anatomy Cell interaction Cell junctions Epithelial cells Genre/Form Laboratory manuals. Manuels de laboratoire. Form Electronic book Author Colgan, Sean P. ISBN 1588295230 9781588295231 9781597451130 1597451134 1280832258 9781280832253 9786610832255 6610832250

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