| Description |
1 online resource (xiv, 265 pages) : illustrations (some color) |
| Series |
Methods in molecular biology, 1064-3745 ; 1691 |
|
Methods in molecular biology (Clifton, N.J.) ; v. 1691
|
| Contents |
Labeling the ER for light and fluorescence microscopy / Chris Hawes, Pengwei Wang, and Verena Kriechbaumer -- 3D electron microscopy of the ER / Maike Kittelmann -- Characterization of proteins localized to plant ER-PM contact sites / Pengwei Wang, Chris Hawes, Christine Richardson, and Patrick J. Hussey -- Preparation and imaging of specialized ER using super-resolution and TEM techniques / Karen Bell, Karl Oparka, and Kirsten Knox -- Quantitation of ER structure and function / Mark Fricker, Luke Heaton, Nick Jones, Boguslaw Obara, Stefanie J. Müller, and Andreas J. Meyer -- Long-term imaging of endoplasmic reticulum morphology in embryos during seed germination / Natasha Dzimitrowicz, Emily Breeze, and Lorenzo Frigerio -- Dancing with the stars : using image analysis to study the choreography of the endoplasmic reticulum and its partners and of movement within its tubules / Lawrence R. Griffing -- Preparation of highly enriched ER membranes using free-flow electrophoresis / Harriet T. Parsons -- ER microsome preparation in Arabidopsis thaliana / Verena Kriechbaumer -- ER membrane lipid composition and metabolism : lipidomic analysis / Laetitia Fouillen, Lilly Maneta-Peyret, and Patrick Moreau -- 2in1 vectors improve in planta BiFC and FRET analyses / Dietmar G. Mehlhorn, Niklas Wallmeroth, Kenneth W. Berendzen, and Christopher Grefen -- Metabolons on the plant ER / Verena Kriechbaumer and Stanley W. Botchway -- Using optical tweezers combined with total internal reflection microscopy to study interactions between the ER and golgi in plant cells / Imogen Sparkes, Rhiannon R. White, Benjamin Coles, Stanley W. Botchway, and Andy Ward -- Protein biosynthesis and maturation in the ER / Emanuela Pedrazzini and Alessandro Vitale -- ER membrane protein interactions using the split-ubiquitin system (SUS) / Lisa Yasmin Asseck, Niklas Wallmeroth, and Christopher Grefen -- Analysis of protein glycosylation in the ER / Jennifer Schoberer, Yun-Ji Shin, Ulrike Vavra, Christiane Veit, and Richard Strasser -- Unfolded protein response / Kazuki Tabara, Yuji Iwata, and Nozomu Koizumi -- Unfolded protein response in Arabidopsis / Cristina Ruberti and Federica Brandizzi -- Fluorescence imaging of autophagy-mediated ER-to-vacuole trafficking in plants / Hadas Peled-Zehavi and Gad Galili -- Imaging the ER and endomembrane system in cereal endosperm / Verena Ibl, Jenny Peters, Eva Stöger, and Elsa Arcalı́s |
| Summary |
This volume presents a range of different techniques that have been used to characterize the structure and function of the endoplasmic reticulum (ER) in higher plants. Chapters guide readers through application of modern microscopy techniques by fluorescence and electron microscopy, new protocols for analysing ER network structure, methods to purify and analyse ER membrane structure and to study protein glycosylation, protocols to study the unfolded protein response, and the role of the ER in autophagy. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Authoritative and cutting-edge, The Plant Endoplasmic Reticulum: Methods and Protocols aims to ensure successful results in the further study of this vital field |
| Bibliography |
Includes bibliographical references and index |
| Notes |
Online resource; title form PDF title page (SpringerProtocols, viewed October 27, 2017) |
| Subject |
Endoplasmic reticulum -- Laboratory manuals
|
|
Plant genetics -- Laboratory manuals
|
|
Endoplasmic reticulum.
|
|
Endoplasmic Reticulum
|
|
Plants
|
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vegetation.
|
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Endoplasmic reticulum
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Plant genetics
|
| Genre/Form |
Laboratory manuals
|
|
Laboratory manuals.
|
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Manuels de laboratoire.
|
| Form |
Electronic book
|
| Author |
Hawes, C. R., editor.
|
|
Kriechbaumer, Verena, 1978- editor.
|
| ISBN |
9781493973897 |
|
1493973894 |
|
1493973886 |
|
9781493973880 |
|
