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Title Epiblast stem cells : methods and protocols / edited by Ramiro Alberio, School of Biosciences, University of Nottingham, Loughborough, UK
Published [Totowa, New Jersey] : Humana Press, [2013]
©2013

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Description 1 online resource (xi, 216 pages) : illustrations (some color)
Series Methods in molecular biology, methods and protocols, 1064-3745 ; 1074
Methods in molecular biology (Clifton, N.J.) ; v. 1074, 1064-3745
Contents Generation and Characterization of Epiblast Stem Cells from Blastocyst-stage Mouse Embryos -- Reversion of Mouse Post-Implantation Epiblast Stem Cells to a Naïve Pluripotent State by Modulation of Signalling Pathways -- Converting Mouse Epiblast Stem Cells into Mouse Embryonic Stem Cells by using Small Molecules -- Isolation and Culture of Rabbit Embryonic Stem Cells -- Goat Embryonic Stem-like Cell Derivation and Characterization -- Derivation and Culture of Canine Embryonic Stem Cells -- Isolation and Culture of Porcine Embryonic Stem Cells -- Isolation and Culture of Pig Epiblast Stem Cells -- Isolation and Culture of Bovine Embryonic Stem Cells -- Analysis of Molecular Markers for Staging Peri-gastrulating Bovine Embryos -- Chicken Embryonic Stem Cells: Establishment and Characterization -- Manipulating the Avian Epiblast and Epiblast-derived Stem Cells -- Stepwise Differentiation from Naïve State Pluripotent Stem Cells to Functional Primordial Germ Cells through an Epiblast-like State -- Isolation and Culture of Porcine Neural Progenitor Cells from Embryos and Pluripotent Stem Cells -- Derivation of Neural Precursors from Bovine Pre-implantation Embryos
Summary In Epiblast Stem Cells: Methods and Protocols, expect researchers in the field provide a detailed collection of techniques and protocols useful to the study of the biology of the pluripotent epiblast. These include methods and techniques used to study epiblast development in different amniotes. This collection brings together contributions from the fields of embryology, stem cell biology and developmental biology together, providing a single volume with detailed procedures for the isolation and culture of epiblasts at different stages of development, and techniques for the study of differentiation into specific lineages
Bibliography Includes bibliographical references and index
Notes English
Subject Embryonic stem cells.
Embryonic stem cells -- Research.
Stem cells.
Anatomy.
Cells.
Germinal layers.
Stem Cells
Embryonic Structures
Publication Formats
Anatomy
Publication Characteristics
Cells
Germ Layers
Embryonic Stem Cells
Laboratory Manuals
anatomy.
Stem cells
Germinal layers
Cells
Anatomy
Embryonic stem cells
Embryonic stem cells -- Research
Genre/Form Laboratory manuals.
Manuels de laboratoire.
Form Electronic book
Author Alberio, Ramiro, editor
ISBN 9781627036283
1627036288
162703627X
9781627036276