Description |
xii, 217 pages : illustrations ; 24 cm |
Series |
Methods in molecular biology ; v. 127 |
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Methods in molecular biology (Clifton, N.J.) ; v. 127
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Contents |
1. The Animal Cap Assay / Jeremy Green -- 2. Cell and Tissue Transplantation in Zebrafish Embryos / Toshiro Mizuno, Minori Shinya and Hiroyuki Takeda -- 3. Ribonuclease Protection Analysis of Gene Expression in Xenopus / Craig S. Newman and Paul A. Krieg -- 4. Quantitative Analysis of mRNA Levels in Xenopus Embryos by Reverse Transcriptase-Polymerase Chain Reaction (RT-PCR) / Oliver C. Steinbach and Ralph A. W. Rupp -- 5. Wholemount In Situ Hybridization of Xenopus and Zebrafish Embryos / Joanne Broadbent and E. Mary Read -- 6. In Situ Hybridization to Sections of Xenopus Embryos / David Bertwistle -- 7. Zebrafish Immunohistochemistry / Rachel Macdonald -- 8. Immunohistochemistry of Xenopus Embryos / Carl Robinson and Matthew Guille -- 9. Preparation and Testing of Synthetic mRNA for Microinjection / Wendy Moore and Matthew Guille -- 10. Microinjection into Xenopus Oocytes and Embryos / Matthew Guille -- 11. Microinjection into Zebrafish Embryos / Qiling Xu |
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12. Expression from DNA Injected into Xenopus Embryos / Ondine Cleaver and Paul A. Krieg -- 13. Promoter Analysis in Zebrafish Embryos / Jos Joore -- 14. Transient Transgenesis in Xenopus laevis Facilitated by AAV-ITRs / Yuchang Fu, Donghui Kan and Sylvia Evans -- 15. Band-Shift Analysis Using Crude Oocyte and Embryo Extracts from Xenopus laevis / Rob Orford and Matthew Guille -- 16. DNA Footprinting Using Crude Embryo Extracts from Xenopus laevis / Rob Orford, Darren Mernagh and Matthew Guille -- 17. Mapping Protein-DNA Interactions Using In Vivo Footprinting / David Warshawsky and Leo Miller |
Notes |
Includes index |
Bibliography |
Includes bibliographical references and index |
Notes |
Also available online via the World Wide Web, by subscription to NeuroSciNow.com via Humana Press |
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Methods in molecular biology (Clifton, N.J.) no:127 1064-3745 |
Subject |
Embryology -- Vertebrates -- Handbooks, manuals, etc
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Embryology -- Vertebrates -- Laboratory manuals
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Vertebrates -- Embryology -- Laboratory manuals.
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Molecular biology -- Technique.
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Xenopus -- Embryos.
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Zebra danio -- Embryos.
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Genre/Form |
Handbooks and manuals.
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Laboratory manuals.
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Author |
Guille, Matthew.
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LC no. |
98050533 |
ISBN |
1592596789 electronic bk |
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0896037908 alkaline paper |
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