| Description |
1 online resource (xx, 369 pages) : illustrations |
| Series |
Methods in molecular biology ; v. 48 |
|
Methods in molecular biology (Clifton, N.J.) ; v. 48.
|
| Contents |
Electroporation theory : concepts and mechanisms / James C. Weaver -- Effects of pulse length and strength on electroporation efficiency / Sek Wen Hui -- Instrumentation / Gunter A. Hofmann -- The introduction of proteins into mammalian cells by electroporation / William F. Morgan, Joseph P. Day -- Electroporation of antigen-presenting cells for T-cell recognition and cytotoxic T-lymphocyte priming / Weisan Chen, James McCluskey -- Electroporation of antibodies into mammalian cells / Paul L. Campbell, James McCluskey, Jing Ping Yeo, Ban-Hock Toh -- Electroporation of adherent cells in situ for the introduction of nonpermeant molecules / Leda H. Raptis, Kevin L. Firth, Heather L. Brownell, Andrea Todd, W. Craig Simon, Brian M. Bennett, Leslie W. MacKenzie, Maria Zannis-Hadjopoulos -- Electrotransformation of Chinese hamster ovary cells / Danielle Gioioso Taghian, Jac A. Nickoloff -- Electroporation of rat pituitary cells / Ruth H. Paulssen, Eyvind J. Paulssen, Kaare M. Gautvik -- Electroporation of plasmid DNA into normal human fibroblasts / F. Andrew Ray -- Electroporation-mediated gene transfer into hepatocytes / Alphonse Cam -- Electroporation of human lymphoblastoid cells / Fen Xia, Howard L. Liber -- The use of electroporated bovine spermatozoa to transfer foreign DNA into oocytes / Marc Gagné, François Pothier, Marc-André Sirard -- Electroporation of embryonic stem cells for generating transgenic mice and studying in vitro differentiation / John S. Mudgett, Thomas J. Livelli -- Electrotransfection with "intracellular" buffer / Maurice J. Hoff, Vincent M. Christoffels, Wil T. Labruyère, Antoon F. Moorman, Wouter H. Lamers -- Effect of cis-located human satellite DNA on electroporation efficiency / Djenann Saint-Dic, Michael S. DuBow -- Quantitation of transient gene expression / Michael K. Showe, Louise C. Showe -- Stable integration of vectors at high copy number for high-level expression in animal cells / James Barsoum -- Electroporation of Drosophila embryos / K. Puloma Kamdar, Thao N. Wagner, Victoria Finnerty -- Transformation of fish cells and embryos / Koji Inoue, Jun-ichiro Hata, Shinya Yamashita -- Electroporation of cardiac cells / Leslie Tung -- Electroporation for gene therapy / Kathryn E. Matthews, Sukhendu B. Dev, Frances Toneguzzo, Armand Keating -- Electrofusion of mammalian cells / Kenneth L. White -- Stabilizing antibody secretion of human Epstein Barr virus-activated B-lymphocytes with hybridoma formation by electrofusion / Susan Perkins, Steven K. Foung -- Electrofusion of mammalian oocytes and embryonic cells / Josef Fulka, Robert M. Moor, Josef Fulka -- Nuclear transfer in bovine embryos / Akira Iritani, Tasuku Mitani -- Electrofusion of mouse embryos to produce tetraploids / Ulrich Petzoldt -- Spectrofluorometric assay for cell-tissue electrofusion / Richard Heller -- Cytometric detection and quantitation of cell-cell electrofusion products / Mark J. Jaroszeski, Richard Gilbert, Richard Heller |
| Summary |
This superb collection of well-tested protocols for the electroporation of proteins and DNA into insect, fish, and mammalian cells also includes proven animal cell electrofusion techniques for studies of somatic cell genetics and development, and for generating monoclonal antibodies. It is distinguished by its coverage of important model cell types from many organisms and tissue types and its detailed instructions for the growth and preparation of specific cells to achieve optimum transfection. Its extensive reference lists, citations of alternative transfer methods, advice on pitfalls to avoid, and descriptions of expected results ensure readily reproducible results even for beginners |
| Analysis |
electroporatie |
|
electroporation |
|
membraanpermeabiliteit |
|
membrane permeability |
|
dierlijke weefsels |
|
animal tissues |
|
Animal Physiology and Biochemistry |
|
Dierfysiologie en -biochemie |
| Bibliography |
Includes bibliographical references and index |
| Notes |
English |
|
Print version record |
| Subject |
Animal cell biotechnology.
|
|
Electrofusion.
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|
Electroporation.
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Cell hybridization.
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Cytology -- Technique.
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Genetics -- Technique.
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Animals
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Electroporation
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Cell Fusion
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Cytological Techniques
|
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Genetic Techniques
|
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Genetics -- Technique
|
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Cytology -- Technique
|
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Cell hybridization
|
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Animal cell biotechnology
|
|
Electrofusion
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Electroporation
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| Genre/Form |
Laboratory manuals.
|
|
Manuels de laboratoire.
|
| Form |
Electronic book
|
| Author |
Nickoloff, Jac A
|
| ISBN |
1592595359 |
|
9781592595358 |
|
9781489940612 |
|
1489940618 |
|
1280836571 |
|
9781280836572 |
|
9786610836574 |
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6610836574 |
|
