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Title Detection of blotted proteins : methods and protocols / edited by Biji T. Kurien, R. Hal Scofield
Published New York, NY : Humana Press, [2015]


Description 1 online resource (xiv, 386 pages) : illustrations (some color)
Series Methods in Molecular Biology, 1940-6029 ; 1314
Methods in molecular biology (Clifton, N.J.) ; v. 1314. 1064-3745
Contents Western blotting : origin and ascent of the species / W. Neal Burnette -- Methods to concentrate proteins for protein isolation, proteomic, and peptidomic evaluation / J.P. Dean Goldring -- Measuring protein concentration on nitrocellulose and after the electrophoretic transfer of protein to nitrocellulose / J.P. Dean Goldring -- Detection of blotted proteins : not all blockers are created equal / Vishal Kothari and Suresh T. Mathews -- Protein stains to detect antigen on membranes / Anil Dsouza and R. Hal Scofield -- Fluorescent labeling of proteins and its application to SDS-PAGE and western blotting / F. Javier Alba, Salvador Bartolomé, Antonio Bermúdez, and Joan-Ramon Daban -- Rapid, antibody-free detection of recombinant proteins on blots using enzyme fragment complementation / Neil W. Charter, Joe Horecka Chin-Yee Loh, Albert Doan, Tom Wehrman, and Keith R. Olson -- Use of nonradioactive detection method for north and south-western blot / Claudia Franke, Daniel Gräfe, Holger Bartsch, and Michael P. Bachmann -- Immunoblotting using radiolabeled reagents for detection / Holger Bartsch, Claudia Franke, and Michael P. Bachmann -- Immunoblotting of antigens : whole, strip, and new-line nitrocellulose membrane immunoblotting using the chemiluminescence technique / Yaser Dorri -- Detection of protein carbonyls by means of biotin hydrazide-streptavidin affinity methods / Kenneth Hensley -- Direct immunodetection of antigens within the precast polyacrylamide gel / Surbhi Desai, Boguslawa R. Dworecki, and Marie C. Nlend -- Quantitative analysis of signal transduction with in-cell western immunofluorescence assays / Vince Boveia and Amy Schutz-Geschwender -- Ultrasensitive protein detection on dot blots and western blots with semiconducting polymer dots / Fangmao Ye, Polina B. Smith, and Daniel T. Chiu -- Co-detection of target and total protein by CyDye labeling and fluorescent ECL plex immunoblotting in a standard proteomics workflow / Caitriona Scaife, Ciara A. McManus, Pamela M. Donoghue, and Michael J. Dunn -- Using biotinylated proteins to demonstrate immunodetection of antigens via western blotting, dot blots, and immunohistochemistry / Thomas Millar, Ronald Knighton, and Jo-Anne Chuck -- Calcium binding by Ro 60 multiple antigenic peptides on PVDF membrane / Biji T. Kurien and Michael P. Bachmann -- Sequential use of immunoblots for characterization of autoantibody specificities / Holger Bartsch and Michael P. Bachmann -- Nanogold immunodetection detection systems for the identification of autoantigens by western blotting / Jacen S. Moore and R. Hal Scofield -- Application of intermittent microwave irradiation to western blot analysis / Yu-Ting Liu and Shinya Toyokuni -- Visualization of unstained protein bands on PVDF / Jun Park, Masaharu Mabuchi, and Ajay Sharma -- Multiplexed fluorescent immunodetection using low autofluorescence Immobilon®-FL membrane / Jun Park, Masaharu Mabuchi, and Ajay Sharma -- Cold microwave-enabled protein detection and quantification / Niels Grützner, Romy M. Heilmann, Aaron G. Smith, Carol B. Johnson, Stanislav Vitha, Jörg M. Steiner, and Andreas K. Holzenburg -- TLC-blot (Far-Eastern Blot) and its application to functional lipidomics / Takao Taki -- Analysis of electroblotted proteins by mass spectrometry / Jose L. Luque-Garcia and Thomas A. Neubert -- On-membrane renaturation of recombinant Ro60 autoantigen by calcium ions / Biji T. Kurien and Michael P. Bachmann -- Phosphoprotein detection on protein electroblot using a phosphate-specific fluorophore / Lee Broderick Bockus and R. Hal Scofield -- Purification of tryptic digests on polyvinylidene difluoride membrane / Biji T. Kurien and R. Hal Scofield -- Detection of blotted proteins on nitrocellulose/PVDF membranes by alta / Jayanta K. Pal, Shilpa J. Rao, and Dhanashri J. Godbole -- Nonstripping "rainbow" and multiple antigen detection (MAD) western blotting / Stan (Stanislaw) Krajewski, Michelle M. Tsukamoto, Xianshu Huang, and Sebastian B. Krajewski -- Supported molecular matrix electrophoresis / Yu-ki Matsuno and Akihiko Kameyama -- Parafilm-M®, an available cost-effective alternative for immuno-blot pouches / Syed M.S. Quadri -- Succinylation-alcian blue staining of mucins on polyvinylidene difluoride membranes / Akihiko Kameyama, Weijie Dong, and Yu-ki Matsuno -- Comparison of chemiluminescence vs. infrared techniques for detection of fetuin-a in saliva / Suresh T. Mathews, Emily Graff, Robert L. Judd, and Vishal Kothari -- Novel methodology for stripping and reprobing of western blots originally developed with colorimetric substrate TMB / Parmita Kar, Saurabh Kumar Agnihotri, Archana Sharma, Rekha Sachan, Madan Lal Bhatt, and Monika Sachdev -- Other notable methods of membrane protein detection : a brief review / Biji T. Kurien and R. Hal Scofield -- Nitrocellulose membrane : the new canvas / Jasmin R. Kurien and Bianca A. Kurien -- Invisible ink marking in ECL membrane assays / Biji T. Kurien
Summary This detailed volume provides methods and techniques for detection after blotting. Chapters guide readers through a number of variations on the theme of protein transfer to solid support followed by detection, presenting adaptations of traditional techniques, and original methods of protein blotting. Written for the Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Practical and authoritative, Detection of Blotted Proteins: Methods and Protocols presents numerous techniques based on the Western blot, providing detailed, readily reproducible methods, tips, and alternatives directly and easily transferable to the laboratory setting
Bibliography Includes bibliographical references and index
Notes Online resource; title from PDF title page (SpringerLink, viewed July 3, 2015)
Subject Immunoblotting -- Laboratory manuals
Electrophoresis -- Laboratory manuals
Electrophoresis -- methods
Blotting, Northern -- methods
Blotting, Southwestern -- methods
Blotting, Western -- methods
Immunoblotting -- methods
Science -- Life Sciences -- Biochemistry.
Genre/Form Laboratory manuals
Laboratory manuals.
Manuels de laboratoire.
Form Electronic book
Author Kurien, Biji T., editor.
Scofield, R. Hal, editor.
ISBN 9781493927180