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Uniform Title DNA electrophoresis (Hanada)
Title DNA electrophoresis : methods and protocols / edited by Katsuhiro Hanada
Published New York, N.Y. : Humana Press, [2020]


Description 1 online resource (1 PDF file, x, 228 pages) : illustrations (some color)
Series Methods in molecular biology, 1940-6029 ; volume 2119
Springer protocols
Methods in molecular biology (Clifton, N.J.) ; v. 2119. 1940-6029
Springer protocols (Series) 1949-2448
Contents Introduction and Perspectives of DNA Electrophoresis -- Two-Dimensional Gel Electrophoresis to Resolve DNA Topoisomers -- Using Two-Dimensional Intact Mitochondrial DNA (mtDNA) Agarose Gel Electrophoresis (2D-IMAGE) to Detect Changes in Topology Associated with Mitochondrial Replication, Transcription, and Damage -- 2D Gel Electrophoresis to Detect DNA Replication and Recombination Intermediates in Budding Yeast -- Neutral-Neutral 2-Dimensional Agarose Gel Electrophoresis for Visualization of E. coli DNA Replication Structures -- Alkali Comet Assay in Genotoxicity Tests -- The Analysis of DNA Interstrand Crosslinks and their Repair by the Modified Comet Assay -- Analysis of Chromosomal DNA Fragmentation in Apoptosis by Pulsed-Field Gel Electrophoresis -- Detection of DNA Damage-Induced DSBs by the Contour-Clamped Homogeneous Electric Field (CHEF) System in Mammalian Cells -- Investigation of DNA Double-Strand Breaks Induced in Host Cells following Infection with Genotoxic Bacteria -- Monitoring of DNA Replication and DNA Double-Strand Breaks in Saccharomyces cerevisiae by Pulsed-Field Gel Electrophoresis (PFGE) -- Pulsed-Field Gel Electrophoresis for Detecting Chromosomal DNA Breakage in Fission Yeast -- Detection of DNA Double-Strand Breaks by Pulsed-Field Gel Electrophoresis of Circular Bacterial Chromosomes -- Detection of Bleomycin-Induced DNA Double-Strand Breaks in Escherichia coli by Pulsed-Field Gel Electrophoresis using a Rotating Gel Electrophoresis System -- Circle-Seq: Isolation and Sequencing of Chromosome-Derived Circular DNA Elements in Cells -- Chromatin Pull-Down Methodology Based on DNA Triple Helix Formation -- DNA Fragments Agarose Gel Electrophoresis for Chromatin Immunoprecipitation (ChIP) -- Post-Labeling/PAGE Method for Detection of DNA Adducts
Summary This volume explores the latest techniques used by researchers to study DNA electrophoresis, with focus on various species including bacteria, yeasts, and mammalian cells. The chapters in this book cover topics such as two-dimensional gel electrophoresis; DNA replication; pulsed-field gel electrophoresis; ChIP; and post-labeling/PAGE method for detection of DNA adducts. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Cutting-edge and authoritative, DNA Electrophoresis: Methods and Protocols is a valuable resource for any researchers looking to learn more about this developing field
Bibliography Includes bibliographical references and index
Notes Description based on online resource; title from PDF title page (viewed October 15, 2021)
Subject Electrophoresis -- Laboratory manuals
Biomedical engineering.
Electrophoresis -- methods
biomedical engineering.
Genre/Form Laboratory manuals
Laboratory manuals.
Manuels de laboratoire.
Form Electronic book
Author Hanada, Katsuhiro, editor
ISBN 9781071603239