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Book Cover
Author Manchenko, Gennady P

Title Handbook of detection of enzymes on electrophoretic gels / Gennady P. Manchenko
Edition 2nd ed
Published Boca Raton : CRC Press, ©2003


Description 1 online resource (553 pages) : illustrations
Contents INTRODUCTION; Enzyme Electrophoresis; Zymogram Techniques; Genetic Bases of Multiple Molecular Forms of Enzymes Developed on Zymograms; Applications; GENERAL PRINCIPLES OF ENZYME DETECTION ON ELECTROPHORETIC GELS; Chromogenic Reactions; Fluorogenic Reactions; Autoradiography; Bioautography; Two-Dimensional Gel Spectroscopy; Immunoblotting; METHODS OF DETECTION OF SPECIFIC ENZYMES; The Structure of Enzyme Sheets; General Considerations, Comments, and Recommendations; Enzyme Sheets; APPENDICES
Summary Still widely used as gene markers, isozymes detected by zymogram techniques have proven valuable in a range of other biological applications over the last few years. Along with these new applications, many new techniques have also emerged. Yet more than eight years since the Handbook of Detection of Enzymes on Electrophoretic Gels was first published, it remains the only book completely devoted to zymogram methods. The time has come to bring its contents up to date. New in the Second Edition: An overview of new applications of enzyme electrophoresis and zymogram techniques | Zymogram techniques for approximately 100 enzymes not included in the first edition, bringing the total to more than 900 methods for detecting more than 400 different enzymes | Information on subunit structure included within the enzyme sheets to facilitate interpretation of isozyme patterns detected on zymograms | An appendix containing information on the buffer systems most commonly used for enzyme electrophoresis in starch, cellulose acetate, and polyacrylamide gels. The second edition of this bestselling handbook makes significant additions to the set of reliable gene markers suitable for electrophoretic analysis. It also strengthens the value of enzyme electrophoresis as a powerful tool proven successful in solving a variety of problems encountered across many biological areas, including the post-genome biology
Bibliography Includes bibliographical references and index
Notes English
Print version record
Subject Enzymes -- Purification -- Handbooks, manuals, etc
Gel electrophoresis -- Handbooks, manuals, etc
Electrophoresis, Polyacrylamide Gel -- methods
Enzymes -- isolation & purification
SCIENCE -- Life Sciences -- Biochemistry.
Enzymes -- Purification
Gel electrophoresis
Eletroforese em gel.
Genre/Form Electronic book
Laboratory manuals
Handbooks and manuals
Handbooks and manuals.
Laboratory manuals.
Manuels de laboratoire.
Guides et manuels.
Form Electronic book
ISBN 9781420040531