| Description |
vii, 436 pages : illustrations ; 28 cm |
| Contents |
Introduction to protein purification strategies -- Preparation of cellular and subcellular extracts -- One-dimensional polyacrylamide gel electrophoresis -- Preparative two-dimensional gel electrophoresis with immobilized PH gradients -- Ion-exchange chromatography -- Size-exclusion chromatography -- Reversed-phase high-performance liquid chromatography -- Affinity and immunoaffinity chromatography -- Metal chelate affinity chromatography -- Multidimensional chromatography of intact proteins -- Identification of protein-protein interactions by conventional column chromatography -- Identification of associated proteins by coimmunoprecipitation -- Chromatin immunoprecipitation of protein complexes -- Identification of protein-protein interactions with glutathione-s-transferase fusion proteins -- Direct visualization of protein interactions in living cells using bimolecular fluorescence complementation analysis -- In silico tools to launch protein interaction analysis : creating focused networks around proteins of interest |
| Summary |
"This new manual is designed for routine, day-to-day use at the bench. By integrating both established in vitro and in vivo molecular techniques with more modern in silico methods, this manual takes the user from the initial steps of obtaining cellular and subcellular extracts, through the purification and isolation steps appropriate for the protein of interest, and, finally, to the steps involved in characterizing and identifying proteins, protein complexes, and protein-protein interactions. These step-wise methods are combined with troubleshooting advice and guidance on selecting an appropriate experimental strategy, making this new manual a handy yet informative resource for bench scientists with all levels of experience."--BOOK JACKET |
| Bibliography |
Includes bibliographical references and index |
| Subject |
Proteins -- Purification -- Laboratory manuals.
|
|
Proteins -- Analysis -- Laboratory manuals.
|
|
Proteins -- isolation & purification.
|
|
Chromatography -- methods.
|
|
Electrophoresis -- methods.
|
|
Immunoprecipitation -- methods.
|
|
Proteins -- analysis.
|
| Genre/Form |
Laboratory Manual.
|
|
Laboratory manuals.
|
| Author |
Simpson, Richard J.
|
|
Adams, Peter D. (Peter David)
|
|
Golemis, Erica.
|
| LC no. |
2008031690 |
| ISBN |
9780879698683 (hardcover : alk. paper) |
|
0879698683 (hardcover : alk. paper) |
|
9780879698676 (paperback: alk. paper) |
|
0879698675 (paperback: alk. paper) |
|
