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Title 2D PAGE : sample preparation and fractionation / edited by Anton Posch
Published Totowa, N.J. : Human Press, ©2008


Description 1 online resource (2 volumes) : illustrations
Series Methods in molecular biology ; v. 424-425
Methods in molecular biology (Clifton, N.J.) ; v. 424-425.
Contents Volume 1. Mechanical/physical methods of cell disruption and tissue homogenization / S. Goldberg -- Bacteria and yeast cell disruption using lytic enzymes / O. Salazar -- Sample solublization buffers for two-dimensional electrophoresis / W. Weiss and A. Gorg -- Quantitation of protein in samples prepared for 2-D electrophoresis / T. Berkelman -- Removal of interfering substances in samples prepared for two-dimensional (2-D) electrophoresis / T. Berkelman -- Protein concentration by hydrophilic interaction chromatography combined with solid phase extraction / U. Schneider -- Difference gel electrophoresis based on LYS/CYS tagging / R. Westermeier and B. Scheibe -- Isotope-coded two-dimensional maps: tagging with deuterated acrylamide and 2-vinylpyridine / P.G. Righetti, R. Sebastiano and A. Citterio -- Stable isotope labeling by amino acids in cell culture (SILAC) / S. Gruhler and I. Kratchmarova -- ICPL--isotope-coded protein label / J. Kellermann -- Radiolabeling for two-dimensional gel analysis / H. Boucherie, A. Massoni and C. Monribot-Espagne -- Sequential extraction of proteins by chemical reagents / S.J. Cordwell -- Reducing sample complexity by RP-HPLC: beyond the tip of the protein expression iceberg / G. Van den Bergh and L. Arckens -- Enriching basic and acidic rat brain proteins with ion exchange mini spin columns before two-dimensional gel electrophoresis / N. Liu and A. Paulus -- Reducing the complexity of the Escherichia coli proteome by chromatography on reactive dye columns / P. Cash and I.R. Booth -- Reducing sample complexity in proteomics by chromatofocusing with simple buffer mixtures / H. Shen, et al. -- Fractionation of proteins by immobilized metal affinity chromatography / X. Sun, J.F. Chiu and Q.Y. He -- Fractionation of proteins by heparin chromatography / S. Xiong, L. Zhang and Q.Y. He -- Fractionation of complex protein mixtures by liquid-phase isoelectric focusing / J. Hey, et al. -- Microscale isoelectric focusing in solution: a method for comprehensive and quantitative proteome analysis using 1-D and 2-D DIGE combined with MicroSol IEF prefractionation / M.J. Han and D.W. Speicher -- Prefractionation, enrichment, desalting and depleting of low volume and low abundance proteins and peptides using the MF10 / V. Wasinger, et al. -- Sample prefractionation in granulated sephadex IEF gels / A. Gorg, C. Luck and W. Weiss -- Free-flow electrophoresis system for plasma proteomic applications / R. Wildgruber, et al. -- Protein fractionation by preparative electrophoresis / M. Fountoulakis and P. Dimitraki -- Isolation of endocitic organelles by density gradient centrifugation / M.E. de Araujo, L.A. Hube and T. Stasyk -- Isolation of highly pure rat liver mitochondria with the aid of zone-electrophoresis in a free flow device (ZE-FFE) / H. Zischka, et al. -- Isolation of proteins and protein complexes by immunoprecipitation / B. Kaboord and M. Perr -- Isolation of phosphoproteins / L.G. Puente and L.A. Megeney -- Glycoprotein enrichment through lectin affinity techniques / Y. Mechref, M. Madera and M.V. Novotny -- Isolation of bacterial cell membranes proteins using carbonate extraction / M.P. Molloy -- Enrichment of membrane proteins by partitioning in detergent/polymer aqueous two-phase systems / H. Everberg, N. Gustavasson and F. Tjerned -- The isolation of detergent-resistant lipid rafts for two-dimensional electrophoresis / K.B. Kim, J.S. Lee and Y.G. Ko -- Isolation of membrane protein complexes by blue native electrophoresis / V. Reisinger and L.A. Eichacker -- Tissue microdissection / H.S. Erickson, J.W. Gillespie and M.R. Emmert-Buck
Volume 2. Application of fluorescence dye saturation labeling for differential proteome analysis of 1,000 microdissected cells from pancreatic ductal adenocarcinoma precursoe lesions / Barbara Sitek [and others] -- Albumin and immunoglobulin depletion of human plasma / Rosalind e. Jenkins [and others] -- Multi-component immunoaffinity subtraction and reversed-phase chromatography of human serum / James Martosella and Nina Zolotarjova -- Immunoaffinity fractionation of plasma proteins by chicken IgY antibodies / Lei Huang and Xiangming Fang -- Proteomics of cerebrospinal fluid methods for sample processing / John E. Hale [and others] -- Sample preparation of bronchoalveolar lavage fluid / Baptiste Leroy, Paul Falmagne, and Ruddy Wattiez -- Preparation of nasal secretions for peoteome analysis / Begona Casado, Paolo Iadarola, and Lewis K. Pannell -- Preparation of urine samples for proteomic analysis / Rembert Pieper -- Isolation of cytoplasmatic proteins from cultures cells for two-dimensional gel electrophoresis / Ying Wang, Jen-Fu Chiu, and Qing-Yu He -- Sample preparation of culture medium from Madin-Darby Canine Kidney cells / Daniel Ambort, Daniel Lottaz, and Erwin Sterchi -- Sample preparation for mass spectromentry analysis of formalin-fixed paraffin-embedded tissue proteomic analysis of formalin-fixed tissue / Nicolas A. Stewart and Timothy D. Veenstra -- Metalloproteomics in the molecular study of cell physiology and disease / Hermann-Josef Thierse, Stefanie Helm, and Patrick Pankert -- Protein extraction from green plant tissue / Ragnar Flengsrud -- The terminator a device for high-throughput extraction of plant material / B.M. van den Berg
Isolation of mitochondria from plant cell culture / Etienne H. Meyer and A. Harvey Millar -- Isolation and preparation of choroplasts from Arabidopsis thaliana plants / Sybille E. Kubis, Kathryn S. Lilley, and Paul Jarvis -- Isolation of plant cell wall proteins / Elisabeth Jamet [and others] -- Isolation and fractionation of the endoplasmic reticulum from castor bean (Ricinius communis) endosperm for proteomic analyses / William J. Simon, Daniel J. Maltman, and Antoni R. Slabas -- Cell wall fractionation for yeast and fungal proteomics / Aida Pitarch, Césa Nombela, and Concha Gil -- Sample preparation procedure for cellular fungi / Alois Harder -- Isolation and enrichment of secreted proteins from filamentous fungi / Martha L. Medina and Wilson A. Frnacisco -- Isolation and solubilization of cellular membrane proteins from bacteria / Kheir Zuobi-Hasona and L. Jeannine Brady -- Isolation and solubilization of gram-positive bacterial cell wall-associated proteins / Jason N. Cole, Steven P. Djordjevic, and Mark J. Walker -- Cell fractionation of parasitic protozoa / Wanderley de Souza, José Andrés-Morgado-Diaz, and Narcisa L. Cunha-e-Silva
Summary The complex nature of proteins often requires a multitude of sample preparation options. In 2D PAGE: Sample Preparation and Fractionation, Volumes 1 & 2, Dr. Anton Posch presents a broad coverage of the principles and recent developments of sample preparation and fractionation tools in Expression Proteomics and specifically for two-dimensional electrophoresis (2-DE). Detailed laboratory methods describe the process of sample fractionation used to reduce the complexity of the protein sample and the methods of 2-DE analysis and a broad variety of gel-free mass spectrometry-based technologies to detect previously undetectable proteins. Vital information regarding protein isolation and preparation for reproducible proteome analysis previously overlooked by scientists has been clearly laid out in this pioneering two-volume set. Volume 1 focuses on the basics of sample preparation and protein labeling techniques with an emphasis on fractionation by chemical reagents, chromatography, and electrophoresis methods. The inclusion of meticulous enrichment strategies further aids the protein researcher. Invaluable for students of Biochemistry, Biomedicine, Biology and Genomics and essential for the practicing scientists, 2D PAGE: Sample Preparation and Fractionation, Volumes 1 & 2 delve into the study of proteins with comprehensive and in depth methods and protocols to provide a greater understanding of this intricate and complicated field
Analysis genexpressie
gene expression
sample processing
biologische technieken
biological techniques
Molecular Biology (General)
Moleculaire biologie (algemeen)
Bibliography Includes bibliographical references and index
Notes Master and use copy. Digital master created according to Benchmark for Faithful Digital Reproductions of Monographs and Serials, Version 1. Digital Library Federation, December 2002. MiAaHDL
Print version record
digitized 2010 HathiTrust Digital Library committed to preserve pda MiAaHDL
Subject Proteomics.
Two-dimensional electrophoresis.
Sample preparation (Chemistry)
Proteomics -- methods
Analytic Sample Preparation Methods
Chemical Fractionation -- methods
Electrophoresis, Gel, Two-Dimensional -- methods
Electrophoresis, Gel, Two-Dimensional
Sample preparation (Chemistry)
Two-dimensional electrophoresis
Genre/Form Laboratory manuals
Laboratory manuals.
Manuels de laboratoire.
Form Electronic book
Author Posch, Anton
ISBN 9781603270649
Other Titles Two-dimensional PAGE sample preparation and fractionation