Title Two-dimensional DNA typing : a parallel approach to genome analysis / André G. Uitterlinden and Jan Vijg

Published New York : E. Horwood, 1994

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Description 1 online resource (xii, 197 pages) : illustrations (some color) Series Ellis Horwood series in molecular biology Ellis Horwood series in molecular biology. Contents Two-dimensional DNA typing: background and technology -- Analysis of DNA: general aspects -- Measuring DNA sequence variation -- DNA sequencing -- Restriction enzyme digestion: RFLP analysis -- Analysis of basepairing -- Evaluation -- Measuring DNA sequence variation on a total genome scale -- Two-dimensional separation of DNA fragments -- Enzymatic digestion as second dimension separation criterion -- DGGE in the second dimension -- Two-dimensional DNA typing -- Summary and discussion: general characteristics of 2D DNA typing Genome analysis: general considerations -- Mapping genomes -- Physical mapping -- Genetic mapping -- DNA markers -- Micro- and minisatellite sequences -- Other interspersed repetitive sequences -- Total genome analysis with DNA markers -- Combining genetic and physical mapping -- Strategies for identifying genetic traits -- Role of two-dimensional DNA typing in genome analysis -- General methodology -- Methods -- DNA isolation -- DNA digestion and sample preparation -- Electrophoretic separations -- Electrotransfer of separation patterns Hybridization analysis -- Experimental considerations -- Denaturing gradient gel constellation -- Buffer dependency of separation patterns -- Reproducibility -- Pattern evaluation by image analysis -- Image capturing -- Spot detection -- Calibration -- Matching -- Database generation, searching and matching -- Applications -- Genome analysis of lower organisms -- Analysis of viral and bacterial genomes -- Analysis of lower eukaryotes -- Genetic linkage analysis -- Locus-specific melting behaviour of minisatellite alleles -- Anatomy of a 2D DNA typing gel -- Association studies Genetic analysis of cancer -- Mutation analysis -- Future developments -- Positional 2D DNA typing: the concerted use of genetic and physical mapping -- Two-dimensional cDNA typing: analysing the expressed part of the genome -- Gene scanning: diagnosis of all possible mutations in disease genes Summary "This book focuses on a recently developed form of DNA analysis, which is based on a combination of two independent gel electrophoretic separation principles. In describing the technology on which the two-dimensional DNA separation principle is based, and in looking at its possible applications, this book is at the forefront of this rapidly emerging area."--Jacket Analysis DNA Analysis Bibliography Includes bibliographical references (pages 164-194) and index Notes Print version record Subject DNA -- Analysis Two-dimensional electrophoresis. DNA -- analysis Electrophoresis, Gel, Two-Dimensional Electrophoresis, Gel, Two-Dimensional -- methods SCIENCE -- Life Sciences -- Genetics & Genomics. DNA -- Analysis Two-dimensional electrophoresis Analyse Genanalyse Methode Sequenzanalyse Chemie DNS ADN -- Analyse. Électrophorèse bidimensionnelle. Genre/Form Genelektrophorese. Form Electronic book Author Vijg, Jan ISBN 0203168712 9780203168714

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